Chloroform anhydrous 99

Ultrapure water (18.2 Ω cm⁻¹) was used for all experiments. D₂O was provided by the ISIS neutron source (Rutherford Appleton Laboratory, Didcot, UK). Buffered solutions were made by dissolving a buffer tablet which was pre-adjusted to pH 7.4 in either H₂O or D₂O (50 mM Tris with 150 mM NaCl). Chloroform anhydrous ≥99% and calcium chloride dihydrate were purchased from Sigma Aldrich and used as received. Hydrogenated and tail deuterated (1,2-dimyristoyl-sn-glycero-3-phosphocholine) DMPC and (1,2-dimyristoyl-sn-glycero-3-phospho-L-serine) DMPS were purchased from Avanti Polar Lipids, US. Protein concentration was used as a measure for the concentration of lipoprotein in solution. Bradford Reagent was used for protein determination as previously detailed^{58 (link)}. Phosphate analysis was performed using the method outlined in Rouser et al.^{59 (link)} and showed for the same protein concentration LDL contained twice the number of phospholipid molecules compared to HDL.

Zinc (II) acetylacetonate monohydrate (Alfa Aesar, Ward Hill, MA, USA), l-tryptophan, l-phenylalanine, 4-pyridinecarboxaldehyde, methanol anhydrous 99.8%, benzene anhydrous 99.8%, chloroform anhydrous 99%, and tetrahydrofuran anhydrous 99.9% were purchased from Sigma-Aldrich, Poland, and potassium hydroxide reagent grade was received from Merck. All these chemicals were used as received.
l-lactide (Forusorb, medical grade) was received from Foryou Medical Device Co., Ltd. Huizhou, Guangdong, China, and before use was purified by recrystallization from dry ethyl acetate.