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Neutravidin protein coated beads

Manufactured by Thermo Fisher Scientific
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NeutrAvidin protein-coated beads are uniform, superparamagnetic particles that are covalently coated with NeutrAvidin, a deglycosylated form of avidin. These beads are designed for use in a variety of affinity-based applications, including protein purification, immunoprecipitation, and cell separation.

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Lab products found in correlation

Anti β actin monoclonal antibody, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

NeutrAvidin beads NeutrAvidin protein NeutrAvidin

2 protocols using neutravidin protein coated beads

1

Biotinylated SNO-Protein Capture and Analysis

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Total biotinylated SNO-proteins were captured by incubation with 50 μl of NeutrAvidin protein-coated beads (Thermo Scientific) at 4°C overnight. The avidin-captured SNO-proteins were eluted from the beads with SDS sample buffer (100 μl) containing 100 mmol/L 2-mercaptoethenol at 37°C for 20 min. Protein samples were separated by 10%–12% SDS-PAGE and then transferred onto polyvinylidene fluoride membranes for immunoblotting with specific antibodies as previously described [21 (link)]. Anti-cofilin-1 (CFL1) antibody was from Abcam (San Francisco, CA). Anti-β-actin monoclonal antibody (1:10 000) was from Ambion (Austin, TX). Antibodies against heat shock protein-70 (HSP70, 1:500) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, 1:500) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Band intensity was quantified by multiplying the absorbance of the surface areas using the NIH ImageJ.
Zhang H.H., Lechuga T.J., Chen Y., Yang Y., Huang L, & Chen D.B. (2016). Quantitative Proteomics Analysis of VEGF-Responsive Endothelial Protein S-Nitrosylation Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and LC-MS/MS. Biology of Reproduction, 94(5), 114.
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2

Biotinylation and Affinity Capture of SNO-Proteins

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SNO-proteins were labeled with biotin-HPDP by BST as previously described (Zhang et al., 2010 (link)). The biotinylated SNO-proteins were captured by NeutrAvidin protein coated beads (Thermo scientific, Rockford, IL) at 4°C overnight. The avidin captured proteins were eluted from with 1x SDS sample buffer containing 100 mM 2-mercaptoethenol at 37°C for 20 min and used for immunoblotting.
ZHANG H.H., WANG W., FENG L., YANG Y., ZHENG J., HUANG L, & CHEN D.B. (2015). S-nitrosylation of Cofilin-1 Serves as a Novel Pathway for VEGF-Stimulated Endothelial Cell Migration. Journal of cellular physiology, 230(2), 406-417.
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