7000gc tq system

Analysis was performed using Agilent 7890B GC system coupled with 7000GC/TQ system connected to PAL RSI85 autosampler (Agilent Technologies, Palo Alto, CA, USA). The column used was HP-5 MS; 30 m × 0.25 mm × 0.25 µm (J&W, Agilent Technologies, Palo Alto, CA, USA) with helium used as a carrier gas at the total flow of 1 mL/min. Chromatographic conditions were as follows: split injection in a ratio 100:1, the injector was set on 250 °C, oven temperature program was: 50 °C held for 1 min, then 4 °C/min up to 130 °C, 10 °C/min to 280 °C, and then isothermal for 2 min. The MS detector operated in the electronic impact ionization mode at 70 eV, transfer line, source, and quadrupole temperatures were set at 320, 230, and 150 °C, respectively. Masses were registered in a range from 30 to 400 m/z. Peaks were identified in MassHunter Workstation Software Version B.08.00 coupled with the NIST17 mass spectra library and accomplished by comparison with linear retention indexes. The relative abundance of each EO constituent was expressed as percentage content based on the peak area normalization. Due to the obtained results, none of the analysed EOs is of pharmacopeial grade. However, the analysis was not performed in accordance with normalization procedure from Polish Pharmacopea XI (different column and different temperature program).