Edwardsiella tarda kctc 12267

Streptococcus iniae KCTC 3657, Vibrio anguillarum KCTC 2711 and Edwardsiella tarda KCTC 12267 were purchased from the Korean Collection for Type Cultures (Daejeon, Korea). For antimicrobial susceptibility tests, strains were cultured on Brain Heart Infusion Agar (BHIA) in an incubator at 25 °t for 24 h. Bacterial colonies taken directly from BHIA plates were incubated in Brain Heart Infusion Broth (BHIB) at 25 ° for 24 h. From this culture, a suspension equivalent to a 0.5 McFarland standard in BHIB was prepared.

Streptococcus iniae KCTC3657, S. parauberis KCTC3651, Edwardsiella tarda KCTC12267, and Aeromonas salmonicida KCCM40239 were purchased from Korean Collection for Type Cultures (Daejeon, Korea) and Korean Culture Center of Microorganisms (Seoul, Korea). Clinical strains S. iniae DSJ19 (Jeju island in Republic of Korea, 1997; used to test the antibacterial activity of 15 extracts from BBD), S. parauberis KSP44 (Jeju island in Republic of Korea, 1999), and S. iniae BS9 (Tongyoung in Republic of Korea, 1998) were originally isolated from a diseased olive flounder and identified by 16s rRNA gene sequencing. After subculturing in brain heart infusion broth (BHIB) for cryopreservation, aliquots of all bacterial strains were kept frozen at −80 °C in BHIB containing 14% glycerol until use.