Mhiib

Manufactured by Merck Group

The MHIIB is a laboratory equipment product from Merck Group. It serves as a core function of performing a specific set of experimental procedures in a controlled laboratory environment. Detailed technical specifications and intended use are not available in this response.

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Lab products found in correlation

Avibactam, by Merck Group (1 mentions) Ca edta, by Merck Group (1 mentions)

2 protocols using mhiib

Antibiotic Susceptibility Testing Protocol

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MIC values of antibiotics were determined by the microdilution method in Mueller Hinton II broth (MHIIB) (Sigma). Susceptibilities were determined in 96-well microtiter plates with an inoculum of 2 × 10⁵ cfu in 200 µL containing two-fold serial dilutions of each compound. The MIC was defined as the lowest concentration of each compound for which no visible growth was observed after 18 h of incubation at 37 °C. Each assay was systematically performed in triplicate. The average of three independent assays was considered in μg/mL.

Dam S., Pagès J.M, & Masi M. (2017). Dual Regulation of the Small RNA MicC and the Quiescent Porin OmpN in Response to Antibiotic Stress in Escherichia coli. Antibiotics, 6(4), 33.

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Antibiotic Stock Solution Preparation

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Imipenem, meropenem, ertapenem, doripenem, ceftazidime–avibactam, aztreonam, and Ca-EDTA (Sigma‒Aldrich) were used to prepare stock solutions <24 h before use. All agents were dissolved in cation-adjusted Müller–Hinton II broth (MHIIB) (Sigma‒Aldrich), and the solutions were sterilized through a syringe filter with a membrane nominal pore size of 0.22 μm. Serial dilutions of the antibiotic and Ca-EDTA stocks were prepared in MHIIB immediately before use.

Wannigama D.L., Sithu Shein A.M., Hurst C., Monk P.N., Hongsing P., Phattharapornjaroen P., Fox Ditcham W.G., Ounjai P., Saethang T., Chantaravisoot N., Wapeesittipan P., Luk-in S., Sae-Joo S., Nilgate S., Rirerm U., Tanasatitchai C., Kueakulpattana N., Laowansiri M., Liao T., Kupwiwat R., Rojanathanes R., Ngamwongsatit N., Tungsanga S., Leelahavanichkul A., Devanga Ragupathi N.K., Badavath V.N., Hosseini Rad S.M., Kanjanabuch T., Hirankarn N., Storer R.J., Cui L., Amarasiri M., Ishikawa H., Higgins P.G., Stick S.M., Kicic A., Chatsuwan T, & Abe S. (2023). Ca-EDTA restores the activity of ceftazidime-avibactam or aztreonam against carbapenemase-producing Klebsiellapneumoniae infections. iScience, 26(7), 107215.

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