Common chemicals and reagents were obtained from Sigma-Aldrich. The components of yeast culturing media as well as LiCl and caffeine were from VWR. Certified molecular biology agarose (BioRad), 1 Kb Plus DNA Ladder (Invitrogen), and GelRed (Biotium) was used in agarose gel electrophoresis. Gene specific oligonucleotide primers were ordered either from Sigma-Aldrich or from Integrated DNA Technology Incorporation. Vector specific primers were provided by UD-GenoMed Medical Genomic Technologies Ltd. Restriction endonucleases and alkaline phosphatase of Promega, together with T4 DNA ligase of Thermo Scientific were used in cloning experiments. Other, more specific materials are described in the next paragraphs.
Common chemicals and reagents
Manufactured by Merck Group
Sourced in Sao Tome and Principe
Common chemicals and reagents are fundamental laboratory supplies used in various scientific and research applications. These substances serve as essential components in experiments, analyses, and chemical reactions. They provide the necessary building blocks and reactants for a wide range of laboratory procedures. While the specific applications and intended uses can vary, these chemicals and reagents play a crucial role in enabling scientific discoveries and advancements.
Automatically generated - may contain errors
Lab products found in correlation
Gelred, by Biotium (1 mentions)
1 kb plus dna ladder, by Thermo Fisher Scientific (1 mentions)
T4 dna ligase, by Thermo Fisher Scientific (1 mentions)
Lithium chloride (licl), by Avantor (1 mentions)
Certified molecular biology agarose, by Bio-Rad (1 mentions)
Mouse anti zo 1, by Thermo Fisher Scientific (1 mentions)
Mouse anti e cadherin, by Merck Group (1 mentions)
Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Rabbit anti apkc, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti rab14, by Merck Group (1 mentions)
2 protocols using common chemicals and reagents
1
Yeast Culturing and DNA Cloning
2
Protein Expression and Localization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Common chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO). ZIP was from AnaSpec (Fremont, CA). The following antibodies were used: rabbit or mouse anti–claudin-1, rabbit or mouse anti–claudin-2, mouse anti–ZO-1, rabbit or mouse anti-occludin from Life Technologies (Grand Island, NY). Rabbit anti-Rab14, mouse anti–β-actin, mouse anti–E-cadherin and rabbit anti–E-cadherin were purchased from Sigma-Aldrich. Rabbit anti-aPKC was from Santa Cruz Biotechnology (Dallas, TX), and mouse anti-PKCι was from BD Biosciences (San Jose, CA). Alexa Fluor secondary antibodies were from Life Technologies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!