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Ultrascan 2k ccd camera

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The Ultrascan 2K CCD camera is a laboratory imaging device. It features a 2048 x 2048 pixel CCD sensor for high-resolution image capture. The camera supports various image acquisition modes and has connectivity options for integration with laboratory equipment.

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Lab products found in correlation

Tecnai 12 transmission electron microscope, by Ametek (4 mentions) Tecnai 12, by Thermo Fisher Scientific (4 mentions) Ultracut uct ultramicrotome, by Leica (2 mentions) Tecnai 12 tem, by Ametek (2 mentions) Tecnai t12, by Thermo Fisher Scientific (2 mentions) Brookhaven 90 plus bi mas instrument, by Brookhaven Instruments (1 mentions) Tecnai t12 transmission electron microscope, by Ametek (1 mentions) Agilent 1100 series, by Agilent Technologies (1 mentions) Hq silver enhancement kit, by Nanoprobes (1 mentions) Nanogold anti rabbit igg, by Nanoprobes (1 mentions) Tecnai 12 transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Ultramicrotome, by Leica (1 mentions) 6520 qtof, by Agilent Technologies (1 mentions) Akta purifier hplc, by GE Healthcare (1 mentions) Superdex 200 10 30 column, by GE Healthcare (1 mentions) Alpha300 m microraman spectrometer, by WITec (1 mentions) Vhx 7000, by Keyence (1 mentions) 100 cx tem, by JEOL (1 mentions) Jem 2100, by JEOL (1 mentions)

Spelling variants of this product

CCD camera (76 citations) Ultrascan CCD camera (46 citations) UltraScan 1000 (2k×2k) CCD camera (12 citations) Ultrascan 2k × 2k CCD camera (8 citations) UltraScan 2k × 2k CCD (8 citations) 2k×2k CCD camera (8 citations) UltraScan 2k × 2k camera (7 citations) UltraScan 2k x 2k CCD camera (5 citations) 2K CCD camera (4 citations) UltraScan 1000 P 2k CCD camera (3 citations)

19 protocols using ultrascan 2k ccd camera

1

Ultrastructural Analysis of Developing Pancreas

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Pancreas tissue was dissected from P0 and P4 animals into fixative (2% glutaraldehyte in 0.1 M Cacodylate buffer (Na(CH3)2AsO2·3H2O), pH7.2), cut into ~1 mm3 sections, and placed at 4 °C overnight. Tissue was washed 3x with 0.1 M Cacodylate buffer, post-fixed with OsO4 in 0.1 M Cacodylate buffer for 30 min and washed three times with 0.1 M Cacodylate buffer. The samples were then dehydrated through 60%, 70%, 80%, 95% ethanol, 100% absolute ethanol (twice), propylene oxide (twice), and were left in propylene oxide/Eponate (1:1) overnight at room temperature in sealed vials. The next day the vials were left open for 2–3 hours to evaporate the propylene oxide. The samples were infiltrated with 100% Eponate and polymerized at ~64 °C for 48 hours. Ultra-thin sections (~70 nm thick) were cut using a Leica Ultra cut UCT ultramicrotome with a diamond knife, picked up on 200 mesh copper EM grids. Grids were stained with 2% uranyl acetate for 10 minutes followed Reynold’s lead citrate staining for 1 minute. Electron microscopy was done on an FEI Tecnai 12 transmission electron microscope equipped with a Gatan Ultrascan 2 K CCD camera.
Serrill J.D., Sander M, & Shih H.P. (2018). Pancreatic Exocrine Tissue Architecture and Integrity are Maintained by E-cadherin During Postnatal Development. Scientific Reports, 8, 13451.
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2

Kidney Ultrastructure Analysis by TEM

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The cortices of the mouse kidney were fixed in 2.5% v/v glutaraldehyde solution for 24 h, then processed for TEM examination according to routine TEM processing and staining protocols for tissues in the City of Hope Electron Microscopy and Atomic Force Microscopy Core13 (link). Ultra-thin sections were cut using a Leica Ultra-cut UCT ultramicrotome. TEM was performed on FEI Tecnai 12 TEM equipped with a Gatan Ultrascan 2 K CCD camera. Average GBM thickness was quantified in ~100 measurements and area of 40 mitochondrion was measured in each group using Image-Pro Premier 9.2 software.
Kato M., Abdollahi M., Tunduguru R., Tsark W., Chen Z., Wu X., Wang J., Chen Z.B., Lin F.M., Lanting L., Wang M., Huss J., Fueger P.T., Chan D, & Natarajan R. (2021). miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1. Communications Biology, 4, 30.
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3

Nanoparticle Characterization Protocol

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Dynamic light scattering (DLS) and ζ potential (ZP) measurements were performed on a Brookhaven 90 Plus/BI-MAS Instrument (Brookhaven Instruments). DLS measurements were obtained by performing 5 runs at 30 s per run, and the ZP values were obtained by measuring 10 runs involving 30 cycles per run. TEM images were obtained with an FEI Tecnai T12 transmission electron microscope at an accelerating voltage of 120 keV, and images were taken with a Gatan Ultrascan 2K CCD camera. NPs dispersed in water at an optimal concentration were drop-cast onto glow-discharged, 300 mesh carbon–Formvar coated grids and allowed to dry before imaging. HPLC analysis was performed by Agilent 1100 Series with UV detector at 220 nm, using a 2.6 μm-C18-100 A° Column and a flow rate of 0.9 mL /min, sample injection volume was 10 μL. Solvents were 0.1% TFA in Water (Solvent A) and 0.1% TFA in Acetonitrile (Solvent B) and the gradient elution method was: 0 min = 75 % Solvent A and 25 % Solvent B, 2 min = 66 % solvent A and 34 % solvent B, 6 min = 39.6 % solvent A and 60.4 % solvent B, 6.01 min to 10 min = 0% solvent A and 100 % solvent B, 10.01 min of 75 % (A) and 25% solvent B. Standard curve was made by measuring the area under PTX peak (at 5 min) at different PTX concentrations.
Tiet P., Li J., Abidi W., Mooney R., Flores L., Aramburo S., Batalla-Covello J., Gonzaga J., Tsaturyan L., Kang Y., Cornejo Y.R., Aboody K.S, & Berlin J.M. (2019). Silica Coated Paclitaxel Nanocrystals Enable Neural Stem Cell Loading For Treatment of Ovarian Cancer. Bioconjugate chemistry, 30(5), 1415-1424.
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4

Transmission Electron Microscopy of Autophagosomes

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Salivary glands were dissected and prepared for imaging as previously described28 (link). Samples were viewed using a FEI Tecnai 12 TEM equipped with a Gatan Ultrascan 2K CCD camera. The number of autophagosomes and lysosomes (Ly) were counted from randomly selected regions and normalized to tissue area (100 μm2).
Morgan-Bathke M., Hill G.A., Harris Z.I., Lin H.H., Chibly A.M., Klein R.R., Burd R., Ann D.K, & Limesand K.H. (2014). Autophagy Correlates with Maintenance of Salivary Gland Function Following Radiation. Scientific Reports, 4, 5206.
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5

Electron Microscopy of WNT5B Knockdown

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The cells infected with shWNT5B or shCtl were collected in 3 days. The electron microscope was done in the core facility at COH following their standard protocol. It has been described in detail elsewhere [28 (link)]. The stained sections were subjected to Electron microscopy, which was done on an FEI Tecnai 12 transmission electron microscope equipped with a Gatan Ultrascan 2 K CCD camera.
Yang L., Perez A.A., Fujie S., Warden C., Li J., Wang Y., Yung B., Chen Y.R., Liu X., Zhang H., Zheng S., Liu Z., Ann D, & Yen Y. (2014). Wnt modulates MCL1 to control cell survival in triple negative breast cancer. BMC Cancer, 14, 124.
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6

Transmission Electron Microscopy of LNPs

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Electron microscopy was performed on an FEI Tecnai 12 transmission electron microscope equipped with a Gatan Ultrascan 2K CCD camera. LNP samples were applied to a glow-discharged 300 mesh Formvar-carbon copper EM grid and stained with 2% uranyl acetate or Nano-W®.
Wong P., Li L., Chea J., Delgado M.K., Crow D., Poku E., Szpikowska B., Bowles N., Channappa D., Colcher D., Wong J.Y., Shively J.E, & Yazaki P.J. (2017). PET Imaging of 64Cu-DOTA-scFv-Anti-PSMA Lipid Nanoparticles (LNPs): Enhanced Tumor Targeting over Anti-PSMA scFv or Untargeted LNPs. Nuclear medicine and biology, 47, 62-68.
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7

Visualizing Mitochondria in Breast Cancer Cells

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MDA-MB-231 control, CBP KD and p300 KD cells were collected, and the cell pellets were fixed with 2% glutaraldehyde in 0.1 M Cacodylate buffer (Na(CH3)2AsO2·3H2O), pH 7.2, at 4 °C overnight. Sample preparation was performed at the electron microscopy and atomic force microscope core at City of Hope. Cell images to visualize the mitochondria were collected on a Tecnai 12 Transmission Electron Microscope equipped with a Gatan UltraScan 2K CCD camera at 1100 times magnification.
Hu X., Ono M., Chimge N.O., Chosa K., Nguyen C., Melendez E., Lou C.H., Lim P., Termini J., Lai K.K., Fueger P.T., Teo J.L., Higuchi Y, & Kahn M. (2021). Differential Kat3 Usage Orchestrates the Integration of Cellular Metabolism with Differentiation. Cancers, 13(23), 5884.
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8

Transmission Electron Microscopy of Spheroids

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Spheroids were fixed with 2.5% glutaraldehyde, 0.1 M cacodylate buffer (Na(CH3)2AsO2 ·3H2O), pH7.2, at 4°C. Standard sample preparation for TEM was followed including post-fixation with osmium tetroxide, serial dehydration with ethanol, and embedding in Eponate.66 (link) Ultra-thin sections (70 nm thick) were acquired by ultramicrotomy, post-stained, and examined on FEI Tecnai 12 transmission electron microscope equipped with a Gatan Ultrascan 2K CCD camera. Immunogold staining was performed using the MAG antibody (Table S1) and Nanogold® anti-rabbit IgG (Nanoprobes). HQ Silver enhancement kit (Nanoprobes) was used to enlarge the Nanogold® staining. Spheroids were processed following standard sample preparation for TEM and imaged.
Feng L., Chao J., Zhang M., Pacquing E., Hu W, & Shi Y. (2023). Developing a human iPSC-derived three-dimensional myelin spheroid platform for modeling myelin diseases. iScience, 26(11), 108037.
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9

Electron Microscopy Sample Preparation Protocol

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Cells grown in T175 flasks were detached by 0.25% trypsin/EDTA treatment, pelleted by centrifugation at 1,200 rpm for 5 min, resuspended in fixative buffer (2% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.2), and then stored at 4 °C until processed using a standard electron microscopy sample preparation protocol (29 (link)). Electron microscopy was performed on an FEI Tecnai 12 transmission electron microscope equipped with a Gatan Ultrascan 2K CCD camera.
Perrigue P.M., Silva M.E., Warden C.D., Feng N.L., Reid M.A., Mota D.J., Joseph L.P., Tian Y.I., Glackin C.A., Gutova M., Najbauer J., Aboody K.S, & Barish M.E. (2015). The Histone Demethylase Jumonji Coordinates Cellular Senescence Including Secretion of Neural Stem Cell-attracting Cytokines. Molecular cancer research : MCR, 13(4), 636-650.
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10

Actin-Myosin Interaction Visualization

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Actin, full-length Vt, and full-length MVt were mixed in KMEI in the indicated ratios and incubated for 15 minutes. 4 µl of sample was applied to a glow-discharged continuous carbon grid (Ted Pella). After 60 s of incubation, the grid was washed in 3 100 µl drops of 1% uranyl acetate and blotted to dryness. Images were collected on a FEI Tecnai 12 operating at 120 kV using an Ultrascan 2K CCD camera (Gatan).
Kim L.Y., Thompson P.M., Lee H.T., Pershad M., Campbell S.L, & Alushin G.M. (2015). The structural basis of actin organization by vinculin and metavinculin. Journal of molecular biology, 428(1), 10-25.
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