All animal procedures were performed according to the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health (19 ), and were approved by the Institutional Review Board of Liaocheng People's Hospital (Liaocheng, China). The rats were housed in a temperature controlled room (temperature, 22±1°C) under a 12 h light/dark cycle with free access to food and water. Male Wistar rats, (n=50; weight, 250–300 g; aged 5–11 weeks) were provided by Shandong Lukang Pharmaceutical Co., Ltd. (Jining, China). The rats were divided into five groups (n=10/group) as follows: i) Sham operation group (sham; administered 0.5 ml normal saline intravenously only); ii) I/R group (administered 0.5 ml normal saline intravenously; other treatment described below); iii) HMGB50 group (50 ng/kg recombinant HMGB1 administered intravenously 30 min before ischemia; the recombinant HMGB1 was purchased in Sino Biological Inc. (Beijing, China; 10326-H08H-50); iv) HMGB100 group (100 ng/kg recombinant HMGB1 administered intravenously 30 min before ischemia); and v) HMGB200 group (200 ng/kg recombinant HMGB1 administered intravenously 30 min before ischemia). All the HMGB groups underwent the process of I/R.
Recombinant hmgb1
Manufactured by Sino Biological
Sourced in China
Recombinant HMGB1 is a laboratory product produced by Sino Biological. It is a recombinant form of the High Mobility Group Box 1 (HMGB1) protein, which is a nuclear protein involved in the regulation of gene expression and DNA repair.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using recombinant hmgb1
1
HMGB1 Pretreatment in Ischemia-Reperfusion Injury
2
CRT and HMGB1 Treatment of Tumor Cells
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Cells were exposed to recombinant CRT (MyBioSource, San Diego, CA) at 3 mg per 106 cells in phosphate buffered saline (PBS) on ice for 30 minutes. Recombinant HMGB1 (Sino Biological Inc. Beijing, China) at 200 ng per mouse was injected along with dying tumor cells.
3
Hippocampal Neuron-Microglial Coculture Protocol
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Mouse hippocampal neuron and microglial cocultures were prepared as previously described [25 (link)]. In brief, embryos were obtained from 18-day gestating mice anaesthetized under pentobarbital sodium. The hippocampus was isolated using sterile micro-forceps under a stereomicroscope and treated with 0.25% trypsin for 15 min at 37 °C. Trypsinization was stopped by the addition of 10% FBS, and cell suspensions were seeded at 1 × 105 cells cm−2 in neurobasal medium (Invitrogen, USA) containing 2% B27 supplement (Invitrogen, USA), 0.5 mM l -glutamine and 25 μM L-glutamic acid. Four days later, half of the medium was replaced with B27/neurobasal medium without l -glutamic acid. The cells were used after 14 days of culture. Recombinant HMGB1 (Sino Biological, Beijing, China) was added to the medium at a concentration of 0.50 ng/mL or 100 ng/mL for 24 h.
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