Log In Sign up
The largest database of trusted experimental protocols

Nk1.1 bv605

Manufactured by BioLegend
Visit Supplier

NK1.1 BV605 is a fluorochrome-conjugated antibody that binds to the NK1.1 antigen. NK1.1 is a cell surface marker expressed on natural killer (NK) cells and a subset of natural killer T (NKT) cells in mice. The BV605 fluorochrome provides a fluorescent signal that can be detected and quantified using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Aria 2, by BD (2 mentions) Epcam apc cy7, by BioLegend (2 mentions) Cd45 bv510, by BioLegend (2 mentions) Cd44 pe im7, by BioLegend (2 mentions) Tryple, by Thermo Fisher Scientific (2 mentions) Aria3 fusion, by BD (2 mentions) Epcam fitc 9c4, by BioLegend (2 mentions) Cd90 pe dazzle thy1, by BioLegend (2 mentions) Cd3 percp, by BioLegend (1 mentions) True nuclear transcription factor buffer set, by BioLegend (1 mentions) Zombie nir fixable viability kit, by BioLegend (1 mentions) Cd8 fitc, by BioLegend (1 mentions) Cd4 apc fire, by BioLegend (1 mentions) Foxp3 alexa fluor 647, by BioLegend (1 mentions) Pd l1 bv421, by BioLegend (1 mentions) Ctla 4 pe cy7, by BioLegend (1 mentions) Cd11b bv570, by BioLegend (1 mentions) Pd 1 bv650, by BD (1 mentions)

3 protocols using nk1.1 bv605

1

Isolation of Murine and Human Intestinal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
After 4 days of murine and 7 days of human co-culture, Matrigel was disrupted and cells were collected into 15ml falcon tubes. For murine ILC1 cultures Matrigel disruption was not required, and cells were gently rinsed from the bottom of the plate using PBS+2%FCS. Samples were rinsed with PBS, then dissociated in TrypLe (Gibco) for 20mins at 37°C. The sorting buffer after this step contained DNAse (250µg/ml), EDTA (1μl/ml), and HEPES (1μl/ml) to maintain single epithelial cells and avoid clumping. Cells were titruated gently, centrifuged and resuspended in sorting buffer. Cells were then filtered (70µm), having pre-coated the filter with sorting buffer to minimize cell loss, and either rinsed with PBS for fixable Live/Dead staining (UV or nearInfraRed, Thermofisher), or stained with EpCAM, CD45, and the requisite combination of antibodies for the experiment, and analyzed (BD Fortessa) or sorted (BD ARIA3 Fusion & BD Aria 2 using BD FACS Diva 8.0.1 software). Isolation of murine IEC and ILC1 following co-culture was performed using EpCAM–APC Cy7 (G8.8, BioLegend), CD45-BV510 (30-F11, bioLegend), NK1.1 BV605 (PK136, BioLegend), CD44-PE (IM7, BioLegend). Isolation of human IEC, FB, and hILC1 used fixable Live/Dead-UV or Live/Dead-nIR CD45-eFluor450(HI30) Invitrogen, EpCAM-FITC (9C4; BioLegend), CD90-PE/Dazzle (Thy1; BioLegend)
Jowett G.M., Norman M.D., Yu T.T., Arévalo P.R., Hoogland D., Lust S.T., Read E., Hamrud E., Walters N.J., Niazi U., Chung M.W., Marciano D., Omer O.S., Zabinski T., Danovi D., Lord G.M., Hilborn J., Evans N.D., Dreiss C.A., Bozec L., Oommen O.P., Lorenz C.D., da Silva R.M., Neves J.F, & Gentleman E. (2020). ILC1 drive intestinal epithelial and matrix remodelling. Nature materials, 20(2), 250-259.
+ Open protocol
+ Expand
2

Isolation of Murine and Human Intestinal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
After 4 days of murine and 7 days of human co-culture, Matrigel was disrupted and cells were collected into 15ml falcon tubes. For murine ILC1 cultures Matrigel disruption was not required, and cells were gently rinsed from the bottom of the plate using PBS+2%FCS. Samples were rinsed with PBS, then dissociated in TrypLe (Gibco) for 20mins at 37°C. The sorting buffer after this step contained DNAse (250µg/ml), EDTA (1μl/ml), and HEPES (1μl/ml) to maintain single epithelial cells and avoid clumping. Cells were titruated gently, centrifuged and resuspended in sorting buffer. Cells were then filtered (70µm), having pre-coated the filter with sorting buffer to minimize cell loss, and either rinsed with PBS for fixable Live/Dead staining (UV or nearInfraRed, Thermofisher), or stained with EpCAM, CD45, and the requisite combination of antibodies for the experiment, and analyzed (BD Fortessa) or sorted (BD ARIA3 Fusion & BD Aria 2 using BD FACS Diva 8.0.1 software). Isolation of murine IEC and ILC1 following co-culture was performed using EpCAM–APC Cy7 (G8.8, BioLegend), CD45-BV510 (30-F11, bioLegend), NK1.1 BV605 (PK136, BioLegend), CD44-PE (IM7, BioLegend). Isolation of human IEC, FB, and hILC1 used fixable Live/Dead-UV or Live/Dead-nIR CD45-eFluor450(HI30) Invitrogen, EpCAM-FITC (9C4; BioLegend), CD90-PE/Dazzle (Thy1; BioLegend)
Jowett G.M., Norman M.D., Yu T.T., Arévalo P.R., Hoogland D., Lust S.T., Read E., Hamrud E., Walters N.J., Niazi U., Chung M.W., Marciano D., Omer O.S., Zabinski T., Danovi D., Lord G.M., Hilborn J., Evans N.D., Dreiss C.A., Bozec L., Oommen O.P., Lorenz C.D., da Silva R.M., Neves J.F, & Gentleman E. (2020). ILC1 drive intestinal epithelial and matrix remodelling. Nature materials, 20(2), 250-259.
+ Open protocol
+ Expand
3

Multiparametric Flow Cytometry of Murine Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood was taken routinely from anesthetized mice (retrobulbar venous plexus). Spleen and tumor tissues were dissociated. Single cells were stained with a panel of conjugated monoclonal antibodies (mAb, 0.125 μg to 1.5 μg each). Zombie NIR™ Fixable Viability Kit by Biolegend (San Diego, United States) staining was performed following the protocol Zombie NIR™ Fixable Viability Kit by Biolegend, extracellular staining was performed following the protocol BD Horizon Briliant Stain Buffer (BD Bioscience), followed by lysis and intracellular staining using the protocol of True-Nuclear™ Transcription Factor Buffer Set by Biolegend. Measurements were performed on a spectral flow cytometer (Cytek™ Aurora). For extracellular stainings Gr1 Alexa Fluor700, CD8 FITC, CD4 APC Fire, CD11b BV570, PD-L1 BV421, NK1.1 BV605, CD19 Spark Blue (Biolegend), CD25 PerCP-eFluor710 (Thermofisher), CD83 BV750, PD-1 BV650 (BD Bioscience) and for intracellular stainings CTLA-4 PE/Cy7, CD3 PerCP, and Foxp3 Alexa Fluor 647 (Biolegend) were used. Data were analyzed using SpectroFlow™ Version 2.2.0.3. and FlowJo™ Version 10.6.1.
Salewski I., Henne J., Engster L., Krone P., Schneider B., Redwanz C., Lemcke H., Henze L., Junghanss C, & Maletzki C. (2022). CDK4/6 blockade provides an alternative approach for treatment of mismatch-repair deficient tumors. Oncoimmunology, 11(1), 2094583.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!