B16f10 melanoma cell line

C57BL/6JJmsSlc mice (6 - 7 weeks old) were purchased from Japan SLC, Inc. (Hamamatsu, Japan). B16F10 melanoma cell line was obtained from ATCC (Manassas, VA, USA). Cells were cultured in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 2 mmol/L L-glutamine.
The mice were inoculated subcutaneously with 3 ×10⁶ B16F10 cells in 100 μL of phosphate-buffered saline (PBS) 3 days before pretreatment PET imaging on Day 0.

The B16F10 melanoma cell line was purchased from ATCC (Manassas, VA, USA) in 2016. Cells were cultured in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 2 mmol/L l-glutamine.

A C57BL/6JolaHsd mouse model inoculated with a poorly immunogenic, Min-expressing, syngeneic tumour cell line (Supplementary Fig. 7a–c) was employed to functionally assess Min(RA)Gag-VLP-induced immune responses. Briefly, a B16F10 melanoma cell line (ATCC, Manassas, VA, USA) was stably transfected with pcDNA3.1-Min and cells were selected with DMEM supplemented with 2 mg/mL geneticin and 10% heat-inactivated FBS (ThermoFisher Scientific). Geneticin-resistant Min-expressing cells were stained with the 10E8 antibody (NIH HIV Reagent Program) at 1 µg/mL and single-cell sorted with a BD FACSAriaIII Cell Sorter (BD) (Supplementary Fig. 7).
C57BL/6JolaHsd mice were immunised twice with purified Gag-VLPs or MinGag-VLPs. Two weeks after the last immunisation, mice were subcutaneously inoculated with 100,000 B16F10Min-expressing cells in the right flank (Fig. 6a). Tumour length and width was measured periodically with a calliper and tumour volume was calculated as follows^{57 (link)}: 0.52*(length*(width²)). Humane endpoint was reached when tumour size was bigger than 1 cm³.

The B16F10 melanoma cell line was purchased from ATCC (Manassas, VA) in 2016. Cells were cultured in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS, Gibco Life Technologies, Grand Island, NY), 1% penicillin-streptomycin, and 2 mmol/L l-glutamine in a humidified incubator at 37°C in an atmosphere of 95% air and 5% carbon dioxide.