AIM-V media and CD4+ and CD8+ Dynal beads were purchased from Invitrogen. RPMI-1640 media and X-Vivo 15 media (BioWhittaker™) were from Lonza Biologics, Inc. Pooled Human AB Serum (PHS) was from Valley Biomedical. Ficoll-Paque Premium was from GE Healthcare Sciences. Aldrithiol-2 (AT-2) and reduced L-Glutathione were from Sigma-Aldrich. Good manufacturing practice-certified (GMP-certified) Interleukin-2 (IL-2), GMP-certified Interleukin-4 (IL-4), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), GMP-certified Anti-CD3 antibody, CliniMACS CD4 and CD14 microbeads, CliniMACS LS Tubing sets, CryoMACS DMSO and CliniMACS PBS/EDTA buffer were from Miltenyi Biotec, Inc. Polyinosinic-polycytidylic acid – poly-L-lysine carboxymethylcellulose (Poly-ICLC) was from Oncovir, Inc. Fluorescent antibodies for IL-2, -IFNγ, -TNFα, -MIP-1β, -CD3, -CD4, and -CD8 were purchased from BD biosciences. Antibodies for CD86, -CD14, -CD11c, and -HLA-DR were purchased from Biolegend. Recombinant human IL-2 and IL-7 were from R&D Systems. The following antibodies were manufactured in-house at the AIDS and Cancer Virus Program, Leidos Biomedical Research, Inc., Frederick National Laboratory: anti-p24CA [94–0001], anti-p17MA [R193244], anti-gp120HIV [P3F5-D5-F8]. Anti-gp41TM(4E10) was obtained from Polymun [AB004].
Cryomacs dmso
Manufactured by Miltenyi Biotec
Sourced in Germany
The CryoMACS DMSO is a laboratory equipment designed for the controlled freezing and storage of cells and cellular products. It provides a consistent and reliable method for the cryopreservation of samples, ensuring the integrity and viability of the frozen material.
Automatically generated - may contain errors
Lab products found in correlation
Aim 5 media, by Thermo Fisher Scientific (1 mentions)
Mip 1β, by BD (1 mentions)
Anti cd3 antibody, by Miltenyi Biotec (1 mentions)
Clinimacs pbs edta buffer, by Miltenyi Biotec (1 mentions)
Tnf α, by BD (1 mentions)
Rpmi 1640 media, by Lonza (1 mentions)
Ficoll paque premium, by GE Healthcare (1 mentions)
Cryovial, by Corning (1 mentions)
2 protocols using cryomacs dmso
1
Reagents and Antibodies for T-cell Culture
2
Cryopreservation of Platelet-Lysate Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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At the fifth subculture, PL-MSCs were harvested at 90% confluency, centrifuged (200 g/5 min), resuspended in cryopreservation media (DMEM-LG + 5% hPL + 10% DMSO (CryoMACS DMSO, MiltenyiBiotec, Germany)), and aliquoted into 2 ml cryovials (Corning, USA). Mr. Frosty container (Nalgene, Thermo Fisher Scientific, USA) was applied for slow freezing of samples according to the manufacturer’s instruction. Then, the cryopreserved PL-MSCs were transferred to the vapor phase of the liquid nitrogen tank (Statebourne Cryogenics, UK).
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