Proteins isolated from cells with NP-40 lysis buffer were resolved using NuPAGE 4–12% Bis-Tris gel (ThermoFisher) and transferred from gel to PVDF membrane using Wet/Tank Blotting Systems (Bio-Rad). Membrane was blocked with 5% non-fat milk in TBSTE buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 0.05% Tween-20, 1mM EDTA), incubated with indicated primary antibodies, followed by secondary antibodies conjugated with horse-radish peroxidase (HRP) and signal was detected with enhanced chemiluminescence reagents (Invitrogen) and X-ray film. Antibodies against ATRX (1:1000, clone D5), FANCD2 (1:1000, clone Fl17) and BLM (1:1000, clone B4) were purchased from Santa Cruz Biotechnology. Antibodies against RNASE H1 (1:1000, NBP2–20171) from Novus biologicals, and WRN (1:1000, clone 8H3) and β-ACTIN HRP (1:5000, clone 13E5) from Cell signaling and DNMT1 (1:1000, ab19905) from Abcam.
Nbp2 20171
Manufactured by Novus Biologicals
NBP2–20171 is a laboratory equipment product offered by Novus Biologicals. It is a device used for laboratory applications, but detailed specifications are not available.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (2 mentions)
Wet tank blotting system, by Bio-Rad (2 mentions)
Dnmt1, by Abcam (2 mentions)
Clone 8h3, by Cell Signaling Technology (2 mentions)
Clone 13e5, by Cell Signaling Technology (2 mentions)
Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (2 mentions)
2 protocols using nbp2 20171
1
Western Blotting of DNA Repair Proteins
2
Western Blotting of DNA Repair Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Proteins isolated from cells with NP-40 lysis buffer were resolved using NuPAGE 4–12% Bis-Tris gel (ThermoFisher) and transferred from gel to PVDF membrane using Wet/Tank Blotting Systems (Bio-Rad). Membrane was blocked with 5% non-fat milk in TBSTE buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 0.05% Tween-20, 1mM EDTA), incubated with indicated primary antibodies, followed by secondary antibodies conjugated with horse-radish peroxidase (HRP) and signal was detected with enhanced chemiluminescence reagents (Invitrogen) and X-ray film. Antibodies against ATRX (1:1000, clone D5), FANCD2 (1:1000, clone Fl17) and BLM (1:1000, clone B4) were purchased from Santa Cruz Biotechnology. Antibodies against RNASE H1 (1:1000, NBP2–20171) from Novus biologicals, and WRN (1:1000, clone 8H3) and β-ACTIN HRP (1:5000, clone 13E5) from Cell signaling and DNMT1 (1:1000, ab19905) from Abcam.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!