Bak sirna

Manufactured by Thermo Fisher Scientific

BAK siRNA is a small interfering RNA (siRNA) product designed to target the BAK gene, which is involved in the regulation of apoptosis. The core function of BAK siRNA is to facilitate the knockdown or silencing of the BAK gene expression, enabling researchers to study its role in cellular processes.

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5 protocols using bak sirna

Transfection of siRNA Targeting Apoptosis

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Transfections were performed as previously described [10 (link)], using either Oligofectamine or Lipofectamine 2000. PMAIP1 (encodes for Noxa) siRNA and BAK siRNA were purchased from Ambion. Two c-myc specific siRNAs were purchased from CST: Cell Signaling Technology, Danvers, MA. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05) and ATF4 (SMARTpool: ON-TARGETplus ATF4 siRNA, L-005125-00-0005) were purchased from Thermo Fisher Scientific.

Ishida C.T., Shu C., Halatsch M.E., Westhoff M.A., Altieri D.C., Karpel-Massler G, & Siegelin M.D. (2017). Mitochondrial matrix chaperone and c-myc inhibition causes enhanced lethality in glioblastoma. Oncotarget, 8(23), 37140-37153.

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Apoptosis Assay with Bim and Bak Knockdown

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Cells were reverse-transfected in six-well plates using RNAiMAX (Thermo Fisher Scientific) with 50 nM siRNA. 48 hr following siRNA treatment, cells were treated for the Cell Event apoptosis assay as indicated and also harvested to verify knockdown by qRT-PCR. BCL2L11 (Bim) siRNA SMARTpool was from Dharmacon (L-004383-00-0005) and BAK siRNA was obtained from Ambion (Life Technologies 4457298).

Kabir S., Cidado J., Andersen C., Dick C., Lin P.C., Mitros T., Ma H., Baik S.H., Belmonte M.A., Drew L, & Corn J.E. (2019). The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. eLife, 8, e44288.

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siRNA Knockdown of BAK and CHOP

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Stable transfected cells were transfected with BAK siRNA (Ambion, Austin, TX), CHOP siRNA (Genechem, Shanghai, China) or negative control siRNA using Lipofectamine RNAiMax Transfection Reagent (Invitrogen) according to manufacturer's instructions. Approximately 72 h later, total proteins were extracted, and the efficiency of siRNA was confirmed by Western blotting.

Kuang Y., He Z., Li L., Wang C., Cheng X., Shi Q., Fu G., Ying J., Tao Q, & Hu X. (2023). The developmental regulator HAND1 inhibits gastric carcinogenesis through enhancing ER stress apoptosis via targeting CHOP and BAK which is augmented by cisplatin. International Journal of Biological Sciences, 19(1), 120-136.

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Transfection of Cell Lines with siRNA

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Transfections were performed as previously described [43 (link)], using either Oligofectamine or Lipofectamine 2000. PMAIP1 siRNA and BAK siRNA were purchased from Ambion. The two Bcl-xL and two c-myc specific siRNAs were purchased from CST: Cell Signaling Technology, Danvers, MA. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05), ATF4 (SMARTpool: ON-TARGETplus ATF4 siRNA, L-005125-00-0005) and four individual Mcl-1 siRNAs (ON-TARGETplus Mcl-1 siRNAs; siRNA-1: GGU UUG GCA UAU CUA AUA A; siRNA-2: GAA GGU GGC AUC AGG AAU G, siRNA-3: GAU UAU CUC UCU CGG UAC CUU, siRNA-4: CGA AGG AAG UAU CGA AUU U (Dharmacon)) were purchased from Thermo Fisher Scientific.

Ishida C.T., Bianchetti E., Shu C., Halatsch M.E., Westhoff M.A., Karpel-Massler G, & Siegelin M.D. (2017). BH3-mimetics and BET-inhibitors elicit enhanced lethality in malignant glioma. Oncotarget, 8(18), 29558-29573.

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Silencing USP9X, BAX, Mcl-1, PMAIP1, and BAK

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SignalSilence^®Usp9X siRNA I #6308 was purchased from CST. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05), BAX (SMARTpool: ON-TARGETplus BAX siRNA, # L-003308-01) and Mcl-1 (SMARTpool: ON-TARGETplus Mcl-1 siRNA, L-004501-00-0005) were purchased from Thermo Fisher Scientific. PMAIP1 siRNA and BAK siRNA were purchased from Ambion. Transfections were performed as previously described [54 (link), 55 (link)]. Briefly, cells were incubated for 6h with the formed complexes of Lipofectamine^®2000 (Invitrogen, Carlsbad, CA) and the respective siRNA (12-well condition) in DMEM without FBS and antibiotics. After 6h, FBS was added to a total concentration of 1.5%.

Karpel-Massler G., Ramani D., Shu C., Halatsch M.E., Westhoff M.A., Bruce J.N., Canoll P, & Siegelin M.D. (2016). Metabolic reprogramming of glioblastoma cells by L-asparaginase sensitizes for apoptosis in vitro and in vivo. Oncotarget, 7(23), 33512-33528.

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