Sc 14245

Manufactured by Santa Cruz Biotechnology

Sc-14245 is a lab equipment product from Santa Cruz Biotechnology. It is a device used for specific applications in research and laboratory settings. No further details on its core function or intended use are provided.

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Lab products found in correlation

Sc 130656, by Santa Cruz Biotechnology (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Ab154154, by Abcam (1 mentions) Estradiol elisa kit, by Cayman Chemical (1 mentions) Duolink in situ red starter kit mouse rabbit, by Merck Group (1 mentions) Sc 1616, by Abcam (1 mentions) Sc 8023, by Santa Cruz Biotechnology (1 mentions) Sc 10790, by Abcam (1 mentions) Lsm 710 confocal microscope, by Zeiss (1 mentions) Anti src, by Merck Group (1 mentions) Anti aromatase, by Santa Cruz Biotechnology (1 mentions)

3 protocols using sc 14245

Western Blot Analysis of Aromatase

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For Western blot analysis, protein samples were boiled with Laemmli loading buffer for 5 min. Equal amounts of protein samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis on a 4–20% Tris-glycine gel, and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore). The membranes were blocked in Odyssey Blocking Buffer for 1 h and incubated with primary antibodies at 4°C overnight. The antibodies against aromatase (sc-14245) and β-actin (sc-130656) were from Santa Cruz Biotechnology, Inc. using a 1:200 dilution. The membrane was then washed with PBS containing 0.1% Tween 20 to remove unbound antibody, followed by incubation with Alexa Fluor 680 goat anti-rabbit IgG or donkey anti-goat IgG for 1 h at room temperature. Bound proteins were visualized using the Odyssey Imaging System (LI-COR Bioscience, Lincoln, NB) and semi-quantitative analyses of the signals were performed with the Image J analysis software (Version 1.30v; NIH, USA). A mean ± SE was calculated from the data for graphical presentation and statistical comparison.

Zhang Q.G., Wang R., Tang H., Dong Y., Chan A., Sareddy G.R., Vadlamudi R.K, & Brann D.W. (2014). Brain-Derived Estrogen Exerts Anti-inflammatory and Neuroprotective Actions in the Rat Hippocampus. Molecular and cellular endocrinology, 389(0), 84-91.

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Protein Interaction and Cytokine Quantification

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Western blotting and coimmunoprecipitation were performed as described previously using specific antibodies against aromatase (#677; kindly provided by Dr. Dean P. Edwards (Baylor College of Medicine), and sc-14245, Santa Cruz Biotechnology), IFI16 (sc-8023, Santa Cruz Biotechnology), Actin (sc-1616), HIF1α (sc-10790), PRMT2 (ab154154, Abcam), and Ifi204 (NBP2-27153, Novus Biologicals) [49 (link)]. To detect protein–protein interactions with high selectivity and sensitivity, PLAs were performed using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich) according to manufacturer’s protocol. Briefly, cells were fixed and incubated with primary antibodies against IFI16 (sc-8023, Santa Cruz Biotechnology), HIF1α (sc-10790), and PRMT2 (ab154154, Abcam). The cells were incubated with PLA probes and the subsequent ligation and rolling circle amplification were performed. The PLA signals were visualized using a Zeiss LSM 710 confocal microscope (Carl Zeiss).
The amounts of IFNα, IFNβ, and IFNγ protein were measured using commercial ELISA kits (MBS2506739, MBS2513798, and MBS2512904, respectively, MyBioSource) according to the manufacturer’s protocol. The amount of estradiol was measured using Estradiol ELISA kit (#582251 and #501890, Cayman) according to the manufacturer’s protocol.

Ka N.L., Lim G.Y., Kim S., Hwang S., Han J., Lee Y.H, & Lee M.O. (2022). Type I IFN stimulates IFI16-mediated aromatase expression in adipocytes that promotes E2-dependent growth of ER-positive breast cancer. Cellular and Molecular Life Sciences, 79(6), 306.

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Quantifying Key Protein Interactions

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anti-ERα (sc-71064, RRID:AB_1122667; 3 μg per 800 μg of total protein) and anti-aromatase (sc-14245, RRID:AB_2088684; 3 μg per 800 μg of total protein), both purchased from Santa Cruz Biotechnology (SCB); anti-Src (Millipore, OP07L-100UG, RRID:AB_10683060; 1 μg per 200 μg of total protein), anti-PP2A (Millipore, 05-421, RRID:AB_309726; 1 μg per 200 μg of total protein).

Storman E.M., Liu N.J, & Gintzler A.R. (2022). Relevance of c-Src and protein phosphatase 2A to aromatase activity: evidence of an acute self-regulating estrogenic signaling complex in rat central nervous system. Journal of neuroendocrinology, 34(4), e13089.

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