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Baf53a

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BAF53A is a protein involved in chromatin remodeling. It is a subunit of the SWI/SNF chromatin remodeling complex. BAF53A plays a role in regulating gene expression.

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Lab products found in correlation

Smarca4, by Abcam (2 mentions) Smarcb1, by Abcam (2 mentions) Baf155, by Cell Signaling Technology (2 mentions) Baf180, by Fortis Life Sciences (2 mentions) Arid1a, by Abcam (2 mentions) Smarca2, by Cell Signaling Technology (2 mentions) Baf60a, by BD (2 mentions) H3k4me1, by Active Motif (2 mentions) H3k27ac, by Active Motif (2 mentions) H3k4me3, by Abcam (2 mentions) Baf170, by Fortis Life Sciences (1 mentions) Las 3000 imaging system, by Fujifilm (1 mentions) Millipore immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) Arid1a, by Santa Cruz Biotechnology (1 mentions)

3 protocols using baf53a

1

Validation of BAF Complex Protein-Protein Interactions

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Co-immunoprecipitation (CoIP) was performed to validate BAF complex protein–protein interaction. Active motif universal magnetic Co-IP kit (Active Motif) was used to make nuclear extract from TC32 cells according to the manufacturer's instructions. The protein G magnetic beads were used for Co-IP and the IP was performed on 300 μg samples using 2 μg of FLI1 polyclonal antibody and rabbit IgG (as a negative control). Western blots were performed individually using the following antibodies: FLI1 (Santa Cruz Biotechnology, C-19 SC365), ARID1A (Santa Cruz Biotechnology, sc-32761), ARID1B (Abcam, ab54761), SMARCA4 (BRG1; Santa Cruz Biotechnology, sc-17796), SMARCC2 (BAF170; Bethyl Laboratories, A301-039A), SMARCC1 (BAF155; Santa Cruz Biotechnology, sc-9746), SMARCB1 (SNF5; Bethyl Laboratories, A301-087A), and ACTL6A (BAF53A; Abcam ab131272). Detection was carried out using Millipore Immobilon Western Chemiluminescent HRP Substrate per the manufacturer's instructions (Millipore Corp.) using a Fujifilm LAS-3000 imaging system.
Selvanathan S.P., Graham G.T., Grego A.R., Baker T.M., Hogg J.R., Simpson M., Batish M., Crompton B., Stegmaier K., Tomazou E.M., Kovar H., Üren A, & Toretsky J.A. (2019). EWS–FLI1 modulated alternative splicing of ARID1A reveals novel oncogenic function through the BAF complex. Nucleic Acids Research, 47(18), 9619-9636.
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2

Chromatin Remodeling Complex Profiling

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SMARCA2 (Cell Signaling 11966) Lot 2; SMARCA4 (Abcam ab110641) - GR150844–42; SMARCB1 (Abcam ab192864) - GR315927–2; BAF53A (Abcam ab3882) - GR95633–1; BAF60A ( BD Biosciences 611728) - 5199785; BAF155 ( Cell Signaling D7F8S) - Lot 2; BAF180 (Bethyl A301–591A) Lot 1; SFPQ ( Genetex GTX114209 ) - 40828; NCL (Bethyl A300–711A) - Lot 1; ARID1A (Abcam ab182560 ) - GR3186289–2; ARID1B (Abcam ab57461) - GR308911–3; ARID2 (ThermoFisher PA5–35857) SF24034426; H3K4me3 (Abcam ab8580); H3K4me1 (Active Motif 39635) 16611004; H3K27ac (Active Motif 39133) 20017049; H3 (epicypher 13–0001) NA gift of Brian Strahl, available from Epicyphyer
Raab J.R., Smith K.N., Spear C.C., Manner C.J., Calabrese J.M, & Magnuson T. (2018). SWI/SNF remains localized to chromatin in the presence of SCHLAP1. Nature genetics, 51(1), 26-29.
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3

Chromatin Remodeling Complex Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
SMARCA2 (Cell Signaling 11966) Lot 2; SMARCA4 (Abcam ab110641) - GR150844–42; SMARCB1 (Abcam ab192864) - GR315927–2; BAF53A (Abcam ab3882) - GR95633–1; BAF60A ( BD Biosciences 611728) - 5199785; BAF155 ( Cell Signaling D7F8S) - Lot 2; BAF180 (Bethyl A301–591A) Lot 1; SFPQ ( Genetex GTX114209 ) - 40828; NCL (Bethyl A300–711A) - Lot 1; ARID1A (Abcam ab182560 ) - GR3186289–2; ARID1B (Abcam ab57461) - GR308911–3; ARID2 (ThermoFisher PA5–35857) SF24034426; H3K4me3 (Abcam ab8580); H3K4me1 (Active Motif 39635) 16611004; H3K27ac (Active Motif 39133) 20017049; H3 (epicypher 13–0001) NA gift of Brian Strahl, available from Epicyphyer
Raab J.R., Smith K.N., Spear C.C., Manner C.J., Calabrese J.M, & Magnuson T. (2018). SWI/SNF remains localized to chromatin in the presence of SCHLAP1. Nature genetics, 51(1), 26-29.
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