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Miseq paired end 150

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The MiSeq (paired-end 150) is a benchtop sequencing system designed for targeted sequencing applications. The instrument uses reversible terminator-based sequencing chemistry to generate up to 15 gigabases of data per run, with read lengths of up to 150 base pairs in paired-end mode.

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Lab products found in correlation

Qiaamp dsp dna blood mini kit, by Qiagen (2 mentions) Dna prep kit, by Illumina (2 mentions) 7500 fast real time pcr instrument, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

MiSeq 2 × 150 bp paired end platform (2 citations) MiSeq 2 × 150 paired-end reads (2 citations) MiSeq paired-end 150-bp sequencing (2 citations)

2 protocols using miseq paired end 150

1

Orthopoxvirus Detection via Real-Time PCR

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A total of 10 clinical specimens consisting of dry swabs from lesion sites were tested. DNA was manually extracted from clinical specimens using the QIAGEN QIAamp DSP DNA Blood Mini Kit. Amplification was achieved by using the Perfecta Multiplex qPCR SuperMix Low Rox PCR assay in conjunction with CDC issued Non-variola Orthopoxvirus Real-Time PCR Primer and Probe Set on the Applied Biosystems 7500 Fast Real-Time PCR instrument. Libraries were prepared for sequencing using the Illumina DNA prep kit. Pooled libraries were sequenced on the Illumina Miseq (paired-end 150), with about 1 million to 2.5 million reads per library.
Chen N.F., Chaguza C., Gagne L., Doucette M., Smole S., Buzby E., Hall J., Ash S., Harrington R., Cofsky S., Clancy S., Kapsak C.J., Sevinsky J., Libuit K., Park D.J., Hemarajata P., Garrigues J.M., Green N.M., Sierra-Patev S., Carpenter-Azevedo K., Huard R.C., Pearson C., Incekara K., Nishimura C., Huang J.P., Gagnon E., Reever E., Razeq J., Muyombwe A., Borges V., Ferreira R., Sobral D., Duarte S., Santos D., Vieira L., Gomes J.P., Aquino C., Savino I.M., Felton K., Bajwa M., Hayward N., Miller H., Naumann A., Allman R., Greer N., Fall A., Mostafa H.H., McHugh M.P., Maloney D.M., Dewar R., Kenicer J., Parker A., Mathers K., Wild J., Cotton S., Templeton K.E., Churchwell G., Lee P.A., Pedrosa M., McGruder B., Schmedes S., Plumb M.R., Wang X., Barcellos R.B., Godinho F.M., Salvato R.S., Ceniseros A., Breban M.I., Grubaugh N.D., Gallagher G.R, & Vogels C.B. (2023). Development of an amplicon-based sequencing approach in response to the global emergence of mpox. PLOS Biology, 21(6), e3002151.
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2

Orthopoxvirus Detection via Real-Time PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
A total of 10 clinical specimens consisting of dry swabs from lesion sites were tested. DNA was manually extracted from clinical specimens using the QIAGEN QIAamp DSP DNA Blood Mini Kit. Amplification was achieved by using the Perfecta Multiplex qPCR SuperMix Low Rox PCR assay in conjunction with CDC issued Non-variola Orthopoxvirus Real-Time PCR Primer and Probe Set on the Applied Biosystems 7500 Fast Real-Time PCR instrument. Libraries were prepared for sequencing using the Illumina DNA prep kit. Pooled libraries were sequenced on the Illumina Miseq (paired-end 150), with about 1 million to 2.5 million reads per library.
Chen N.F., Chaguza C., Gagne L., Doucette M., Smole S., Buzby E., Hall J., Ash S., Harrington R., Cofsky S., Clancy S., Kapsak C.J., Sevinsky J., Libuit K., Park D.J., Hemarajata P., Garrigues J.M., Green N.M., Sierra-Patev S., Carpenter-Azevedo K., Huard R.C., Pearson C., Incekara K., Nishimura C., Huang J.P., Gagnon E., Reever E., Razeq J., Muyombwe A., Borges V., Ferreira R., Sobral D., Duarte S., Santos D., Vieira L., Gomes J.P., Aquino C., Savino I.M., Felton K., Bajwa M., Hayward N., Miller H., Naumann A., Allman R., Greer N., Fall A., Mostafa H.H., McHugh M.P., Maloney D.M., Dewar R., Kenicer J., Parker A., Mathers K., Wild J., Cotton S., Templeton K.E., Churchwell G., Lee P.A., Pedrosa M., McGruder B., Schmedes S., Plumb M.R., Wang X., Barcellos R.B., Godinho F.M., Salvato R.S., Ceniseros A., Breban M.I., Grubaugh N.D., Gallagher G.R, & Vogels C.B. (2023). Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus. medRxiv.
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