Cells sorting was confirmed by flow cytometry prior to use. Harvested and sorted cells were suspended to a concentration of 1×106 cells/mL in cold PBS, 10% FCS, and 1% sodium azide. 10 μg/mL of FITC conjugated anti-CD31 antibody (Abcam, Cambridge, UK) was added to the suspension and incubated for 30 min in the dark, at room temperature. Cells were then washed 3 times by centrifugation at 400 × g for 5 min before being resuspended. Flow cytometry was performed on a BD FACSCelesta.
Fitc conjugated anti cd31 antibody
Manufactured by Abcam
Sourced in United Kingdom
FITC conjugated anti-CD31 antibody is a fluorescently labeled antibody that binds to the CD31 (PECAM-1) cell surface antigen. CD31 is expressed on endothelial cells and is commonly used as a marker for the identification and quantification of these cells.
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Lab products found in correlation
2 protocols using fitc conjugated anti cd31 antibody
1
Flow Cytometry-Based Cell Sorting
2
Isolation of DA-Endothelial Cells by FACS
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DA-endothelial cells (DA-ECs) were isolated by fluorescence-activated cell sorting (FACS).23 (link) DA tissues from ≈30 rat fetuses on gestational day 21 were subjected to FACS to obtain one sample. We obtained 6 samples for quantitative reverse transcription polymerase chain reaction. Dispersed cells by collagenase enzyme mixture were subjected to FACS analysis. Fluorescein isothiocyanate (FITC)–conjugated anti-CD31 antibody (Abcam, Cambridge, United Kingdom) and APC/Cy7–conjugated anti-CD45 antibody (BioLegend, San Diego, MA) were used as cell surface markers for ECs and hematopoietic derivation cells, respectively. CD31+/CD45− and CD31−/CD45− were defined as DA-ECs and DA-non-ECs. This analysis was conducted using BD FACS AriaII (Becton Dickinson, San Jose, CA).
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