Powerplex 1

Manufactured by Promega

The PowerPlex 1.2 is a multiplex STR (short tandem repeat) amplification kit developed by Promega. It is designed for human identification applications, enabling simultaneous amplification and detection of multiple STR loci.

Automatically generated - may contain errors

Lab products found in correlation

Cellid str, by Promega (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Ae buffer, by Qiagen (1 mentions) Qiaamp dna mini kit, by Qiagen (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Iscove s modified dulbecco s medium, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Glutamax supplement, by Thermo Fisher Scientific (1 mentions) Rd es, by American Type Culture Collection (1 mentions) Sk es 1, by American Type Culture Collection (1 mentions) Sk n mc, by American Type Culture Collection (1 mentions) We 68, by American Type Culture Collection (1 mentions) Ttc 466, by American Type Culture Collection (1 mentions) Vh 64, by American Type Culture Collection (1 mentions) Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Merck Group (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Cosmic calf serum, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PowerPlex 1.2 kit PowerPlex 1.2 microsatellite detection kit PowerPlex 1.2 System PowerPlex VR 1.2 system GenePrint PowerPlex 1.2 system

9 protocols using powerplex 1

Cell Line DNA Fingerprinting

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DNA was isolated using a QIAamp DNA mini kit from 5–6 × 106 cells was isolated (Qiagen, Valencia, CA) following the manufacturer’s protocol. The isolated DNA was eluted in elution buffer (100μl) (Buffer AE, Qiagen). The concentration of the DNA and it purity was measured by Nanodrop. Cell line authentication was done by using DNA (50ng) for DNA fingerprint analysis of short tandem repeat profiling (PowerPlex 1.2, Promega, Madison, WI).
Fingerprinting results for each cell line were compared to reference fingerprints provided by Dr. Minna or the ATCC.

Sen T., Rodriguez B.L., Chen L., Corte C.D., Morikawa N., Fujimoto J., Cristea S., Nguyen T., Diao L., Li L., Fan Y., Yang Y., Wang J., Glisson B.S., Wistuba I.I., Sage J., Heymach J.V., Gibbons D.L, & Byers L.A. (2019). Targeting DNA damage response promotes anti-tumor immunity through STING-mediated T-cell activation in small cell lung cancer. Cancer discovery, 9(5), 646-661.

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Isolation and Characterization of MCL Cells

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Thirty-two patients with MCL were diagnosed according to WHO lymphoma classification [48 ]. The study was performed in accordance with protocols approved by the local ethical committee, and all patients gave their informed consent. Mononuclear cells of 6 patients (Supplementary Table S1) were isolated from unicellular suspension obtained from mechanically minced lymph nodes or spleen. Cells were re-suspended and enriched MCL samples (>70% MCL) were cryopreserved in 10% DMSO until further study. Mino, SP53 and Jeko-1 cell lines, all carrying the t(11;14)(q13;q32) translocation, were generously contributed by Dr. Raymond Lai, University of Alberta and Cross Cancer Institute (Edmonton, Alberta, Canada). Z-138 cell line was generously contributed by Dr Bertoni F., IOSI Oncology Institute of Southern Switzerland, (Bellinzona, Switzerland). Cell lines were authenticated in our lab by fingerprinting (Power Plex 1.2, Promega) in January 2011 and in March 2015. All cells were cultured in RPMI 1640 supplemented with 10% heat-inactivated fetal calf serum (FCS; Lonza), 100 U/ml penicillin, 100 μg/ml streptomycin, and 20 mM L-glutamine (Sigma), and maintained in a humidified 5% CO₂ incubator at 37°C.

Mastorci K., Montico B., Faè D.A., Sigalotti L., Ponzoni M., Inghirami G., Dolcetti R, & Col J.D. (2016). Phospholipid scramblase 1 as a critical node at the crossroad between autophagy and apoptosis in mantle cell lymphoma. Oncotarget, 7(27), 41913-41928.

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Ewing Sarcoma Cell Lines Comprehensive Characterization

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Ewing sarcoma cell lines (n = 21) were obtained from multiple sources: L-1062 and L-872 were established in-house [21 (link)]; CHP100, RM-82, IARC-EW7, TC32 and 6647, CHP100, RM-82, IARC-EW-7, WE-68, IARC-EW-3, STA-ET-2.1, TTC-466, STA-ET-10, CADO-ES1, TC-71, VH-64, COH and STA-ET-1 were obtained from the EuroBoNeT consortium collection (Institute of Pathology, University Medical Center, Düsseldorf, Germany) [22 (link)] and SK-ES-1, SK-NM-C, A-673 and R-D-ES from the American Type Culture Collection (ATCC). All cell lines and primary culture L-4027 were cultured in a monolayer under equal conditions and in Iscove’s modified Dulbecco’s medium containing GlutaMAX supplement, supplemented with 1 % streptomycin/penicillin and 10 % heat-inactivated FCS (all from Life Technologies, Bleiswijk, The Netherlands). Authentication of cell lines using Powerplex 1.2 and CellID STR (Promega, Leiden, the Netherlands) and mycoplasma DNA Q-PCR screening were regularly performed on all cell lines.

Sand L.G., Berghuis D., Szuhai K, & Hogendoorn P.C. (2016). Expression of CCL21 in Ewing sarcoma shows an inverse correlation with metastases and is a candidate target for immunotherapy. Cancer Immunology, Immunotherapy, 65, 995-1002.

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Establishment and Authentication of NSCLC Cell Lines

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NSCLC cell lines were established by Dr J.D.Minna and Dr A. Gazdar at the National Cancer Institute and the University of Texas Southwestern Medical Center (Dallas, TX) or were obtained from ATCC. They were maintained in RPMI-1640 (R8758, Sigma Life Science), supplemented with glutamine, 10% heat-inactivated fetal bovine serum (Gibco) and 1% Penicillin/Streptomycin (Sigma Life Science) in a humidified chamber at 5% CO2. All cell lines were authenticated between 2009 and 2011 using STR profiling (PowerPlex 1.2, Promega, Madison, WI) for at least eight different loci and results were compared to reference STR profiles available through ATCC or provided by Dr Minna at UTSW. Following authentication, cell line stocks were frozen and maintained in liquid nitrogen until they were used in the reported experiments. All cell lines were mycoplasma- tested prior to experiments.

Skoulidis F., Byers L.A., Diao L., Papadimitrakopoulou V.A., Tong P., Izzo J., Behrens C., Kadara H., Parra E.R., Canales J.R., Zhang J., Giri U., Gudikote J., Cortez M.A., Yang C., Fan Y.H., Peyton M., Girard L., Coombes K.R., Toniatti C., Heffernan T.P., Choi M., Frampton G.M., Miller V., Weinstein J.N., Herbst R.S., Wong K.K., Zhang J., Sharma P., Mills G.B., Hong W.K., Minna J.D., Allison J.P., Futreal A., Wang J., Wistuba I.I, & Heymach J.V. (2015). Co-occurring genomic alterations define major subsets of KRAS - mutant lung adenocarcinoma with distinct biology, immune profiles, and therapeutic vulnerabilities. Cancer discovery, 5(8), 860-877.

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Cancer Cell Lines Characterization

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Non-small cell lung cancer (NSCLC) cells A549, H2009, HCC 2429, HCC 4017, H23, and HCC 15 were supplied by John Minna (Hamon Cancer Center, UT Southwestern Medical Center, Dallas, TX). The breast cancer cell line MDA-MB-231 was kindly provided by Michael White (Department of Cell Biology, University of Texas Southwestern Medical School, Dallas, TX). All cancer cell lines were cultured in basal medium supplemented with 10% Cosmic Calf Serum (Thermo Scientific) at 37°C in 5% CO₂. All cell lines used in the present studies were mycoplasma free (e-Myco kit, Boca Scientific) and DNA fingerprinted (PowerPlex 1.2, Promega). All cells were compared to the complete database in our own collection and to that of ATCC. All cell lines are commercially available through the ATCC Cell Systems (Gaithersburg, MD).

El-Ashmawy M., Delgado O., Cardentey A., Wright W.E, & Shay J.W. (2014). CDDO-Me Protects Normal Lung and Breast Epithelial Cells but Not Cancer Cells from Radiation. PLoS ONE, 9(12), e115600.

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Proliferation Assays with TLR Ligands

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Mino, SP53, and Jeko-1 cell lines were contributed by Dr. Raymond Lai. Cell lines were authenticated by fingerprinting (Power Plex 1.2, Promega) in January 2012. All cells were cultured in RPMI-1640 supplemented with 10% FBS heat-inactivated, 100 U/ml penicillin, 100 μg/ml streptomycin and 20 mM L-glutamine. Proliferation experiments were performed after an overnight starvation (2% FBS), with or without 30 ng/ml TLR1/2 ligand, or 150 ng/ml TLR5 ligand.

Mastorci K., Muraro E., Pasini E., Furlan C., Sigalotti L., Cinco M., Dolcetti R, & Fratta E. (2016). Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma. PLoS ONE, 11(4), e0153823.

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Cell Line Characterization by STR

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LY2, MCF7, CAMA-1, MDA-MB-175, MDA-MB-361, MDA-MB-231 and MDA-MB-435 cell lines were cultured in DMEM. BT474, T47D, ZR75B, MPE600, HCC1428 cell lines were cultured in RPMI. All cell lines were obtained as a kind gift from Dr. Joe Gray, (Oregon Health Sciences University, USA), and maintained with 10% Fetal Bovine Serum (FBS) at 37 °C in 5% CO₂ and 21% O₂. All cell lines were verified by short tandem repeat (STR) genotyping. Genomic DNA was extracted by Wizard SV Genomic DNA purification system (Promega). STR profiles were compared with publically available profiles using Promega Powerplex 1.2.

Padró M., Louie R.J., Lananna B.V., Krieg A.J., Timmerman L.A, & Chan D.A. (2017). Genome-independent hypoxic repression of estrogen receptor alpha in breast cancer cells. BMC Cancer, 17, 203.

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Cell Culture and Authentication Protocol

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Wild-type HCT116 and Staufen1 knockout (KO) HCT116 cells were cultured in RPMI media (Welgene) supplemented with 10% Fetalgro bovine growth serum (RMbio). KG-1 cells were grown in RPMI-1640 (Welgene) supplemented with 10% fetal-bovine serum (Thermo Fisher). All cells were incubated at 37 °C in a humidified atmosphere of 5 % CO2. All cell lines used in this study were male. All cell lines were authenticated using short tandem repeat (STR) profiling(PowerPlex 1.2; Promega), and results were compared with reference STR profiles available through the ATCC.

Ku Y., Park J., Cho R., Lee Y., Park H., Kim M., Hur K., Byun S.Y., Liu J., Shum D., Shin D., Koh Y., Cho J., Yoon S., Hong J., & Kim Y. (2020). Non-canonical immune response to the inhibition of DNA methylation via stabilization of endogenous retrovirus dsRNAs.

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Authentication of Ewing Sarcoma Cell Lines

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21 Ewing sarcoma cell lines were obtained from multiple sources: L-1062 and L-872 were established 1: event reported or 0: no event reported. c 1 tumour volume > 200 ml or 0: < 200 ml. h 1: < 10% tumour vitality or 0 > 10% tumour vitality. [22] and authentication of cell lines using Powerplex 1.2 and CellID STR (Promega, Leiden, The Netherlands) were performed on all cell lines.

Sand L.G., Scotlandi K., Berghuis D., Snaar-Jagalska B.E., Picci P., Schmidt T., Szuhai K, & Hogendoorn P.C. (2015). CXCL14, CXCR7 expression and CXCR4 splice variant ratio associate with survival and metastases in Ewing sarcoma patients. European journal of cancer (Oxford, England : 1990), 51(17).

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