C57Bl6;Tg(SNCA*E35K-E46K-E61K)3KL-3798 (homozygous), B6N.Cg-Tg (SNCA*E46K) 3Elan/J (heterozygous), C57Bl6;Tg (SNCA*WT)WT-3877 (heterozygous), C57BL/6NCrl, and C57BL/6NTac SNCA KO (homozygous) mice were either purchased from The Jackson Laboratory (E46K: JAX #018768; JAX: 3KL-032799)Charles River (C57Bl6; #027) or generated in house (Line WT-3877). All mice lines were immunocompetent and were housed under standard specific pathogen-free conditions and maintained at the Hale BTM facility in accordance with National Institutes of Health guidelines on use of laboratory animals and an approved protocol by the Brigham and Women’s Hospital (IACUC protocol #2016000314). Mice were kept in normal 12 h light/12 h dark cycles and had free access to food and water. Because of the selective more relevant phenotype that we have previously observed in 3KL+/+ (Fanning et al., 2019 (link); Nuber et al., 2018 (link); Rajsombath et al., 2019 (link)), all mice in this study were males and age matched at 12 months.
C57bl 6ncrl
Manufactured by Jackson ImmunoResearch
C57BL/6NCrl is a mouse strain commonly used in biomedical research. It is a substrain of the well-known C57BL/6 mouse, which is one of the most widely used laboratory mouse models. The C57BL/6NCrl strain is maintained by Charles River Laboratories.
Automatically generated - may contain errors
Lab products found in correlation
B6n cg tg snca e, by Jackson ImmunoResearch (2 mentions)
K 3elan j, by Jackson ImmunoResearch (2 mentions)
C57bl 6ntac snca, by Jackson ImmunoResearch (2 mentions)
C57bl 6 tg, by Jackson ImmunoResearch (2 mentions)
C57bl 6, by Charles River Laboratories (2 mentions)
C57bl 6j, by Charles River Laboratories (2 mentions)
Balb c, by Charles River Laboratories (2 mentions)
Microhematocrit capillary tube, by Thermo Fisher Scientific (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)
6 protocols using c57bl 6ncrl
1
Murine Models of Synucleinopathy
2
Lipoprotein(a) Clearance in Sort1 Knockout Mice
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Mice bearing a Sort1 null allele (48 (link)) on a C57BL/6NCrl background were sourced from Valerie Wallace (Ottawa Hospital Research Institute) and were backcrossed onto C57BL/6J (Jackson Laboratories) over eight generations. Mice were housed in a 12 h-light/dark cycle and fed chow diet (2018 Teklad Global; Harlan Laboratories). The IRCM animal care committee approved all procedures. Heterozygous animals were intercrossed to yield Sort1+/+ and Sort1-/- littermates that were used in clearance experiments. After 3 h fasting with a free access to water, mice were injected in the tail vein with 25 μg of human Lp(a) in 100 μl of saline. Each mouse was bled after injection at the tip of the tail at 5, 15, 30, 120, and 360 min into heparin-coated capillaries (22-362-566, Microhematocrit Capillary Tubes, Fisher Scientific). For Lp(a) injections, food was returned after 120 min. Blood was transferred to Eppendorf tubes on ice and centrifuged at 3800 g for 10 min at 4°C. Plasma was assessed immediately or stored at −80°C. Plasma Lp(a) concentrations were determined by an Lp(a) ELISA kit (Mercodia).
3
Mouse Strain Characterization for Immunology
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Mice were maintained at Yale University in accordance with the
guidelines of the Institutional Animal Care and Use Committee (IACUC). The
following mouse strains were used this study: WT C57BL/6 (Charles River,
#C57BL/6NCrl or Jackson Labs, C57BL/6J #000664), Balb/c (Charles River, #
Balb/cAnNCrl), Il18bp−/− and
Il18r1−/− (Yale, R. Flavell),
Rag2−/− (Yale, D. Schatz),
XCR1DTR and TCRP14 Transgenic (Yale,
A. Iwasaki) and Perforin−/− (Yale, M.
Bosenberg). Experiments were conducted using age- and gender-matched mice in
accordance with approved institutional protocols.
guidelines of the Institutional Animal Care and Use Committee (IACUC). The
following mouse strains were used this study: WT C57BL/6 (Charles River,
#C57BL/6NCrl or Jackson Labs, C57BL/6J #000664), Balb/c (Charles River, #
Balb/cAnNCrl), Il18bp−/− and
Il18r1−/− (Yale, R. Flavell),
Rag2−/− (Yale, D. Schatz),
XCR1DTR and TCRP14 Transgenic (Yale,
A. Iwasaki) and Perforin−/− (Yale, M.
Bosenberg). Experiments were conducted using age- and gender-matched mice in
accordance with approved institutional protocols.
4
Murine Models of Synucleinopathy
5
Generation of Trp53 Overexpression Cell Lines
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Wild-type (wt)-Trp53 and GOF mutant Trp53R270H cDNAs were amplified and subcloned from wild-type C57BL/6NCrl (Jackson Laboratory) and 129S4-Trp53tm3Tyj/Nci mouse liver cDNAs, respectively. These were subcloned into the pPB-CAG-IB PiggyBac transposon expression vector (a gift from Dr. Hitoshi Niwa) and cotransfected with the transposase expression vector using Lipofectamine LTX (Thermo Fisher Scientific). Two cell lines were established for each expression vector by independent transfection experiments.
6
Mouse Strain Characterization for Immunology
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