Consistent with the smoking method in Section 3.9.1 , we changed the artificial saliva to dichloromethane to collect the flue gas. After smoking, the Cambridge filter and dichloromethane were transferred into a conical flask. Aroma components were extracted at 10 °C for 3 h, and the volume of the extract was maintained constant at 100 mL. The internal standard heptadecane was added to the extraction solution, blown with nitrogen, and dried with anhydrous magnesium sulfate. The extract was filtered through a 0.45μm microporous organic filter membrane for GC-MS.
Chromatographic analysis was performed using a QP2010 series GC–MS (Agilent Corporation, Palo Alto, CA, USA) equipped with an Agilent 19091S column (60 m × 0.25 mm × 0.25 μm). The injection volume was 0.2 μL. The injection temperature was 250 °C. All samples were injected in split mode with a split ratio of 5:1 and a flow rate of 1.0 mL/min. The initial temperature was maintained at 50 °C for 4 min and then raised to 300 °C at a rate of 4 °C/min and maintained for 10 min. The solvent delay time was 6 min. Mass spectra were acquired in full-scan mode with repetitive scanning from 30 m/z to 300 m/z in 1 s. The mass spectrum transmission line temperature was 280 °C. The ion source temperature was 230 °C. The electronic ionization (EI) source energy was 70 eV, and the quadrupole temperature was 150 °C.
Chromatographic analysis was performed using a QP2010 series GC–MS (Agilent Corporation, Palo Alto, CA, USA) equipped with an Agilent 19091S column (60 m × 0.25 mm × 0.25 μm). The injection volume was 0.2 μL. The injection temperature was 250 °C. All samples were injected in split mode with a split ratio of 5:1 and a flow rate of 1.0 mL/min. The initial temperature was maintained at 50 °C for 4 min and then raised to 300 °C at a rate of 4 °C/min and maintained for 10 min. The solvent delay time was 6 min. Mass spectra were acquired in full-scan mode with repetitive scanning from 30 m/z to 300 m/z in 1 s. The mass spectrum transmission line temperature was 280 °C. The ion source temperature was 230 °C. The electronic ionization (EI) source energy was 70 eV, and the quadrupole temperature was 150 °C.