Rpmi 1640 nutrient medium

Manufactured by Merck Group

Sourced in United States

RPMI 1640 is a widely used cell culture medium formulated to support the growth of a variety of mammalian cell lines. It provides the necessary nutrients and growth factors for cell proliferation and maintenance in vitro.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Ht 29, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Caco 2, by Leibniz Institute DSMZ (1 mentions) Non essential amino acids (neaa), by Biowest (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Acarbose, by Merck Group (1 mentions) Alginic acid sodium salt, by Thermo Fisher Scientific (1 mentions) α glucosidase, by Merck Group (1 mentions) Gallic acid, by Merck Group (1 mentions) Folin ciocalteu s reagent, by Carlo Erba (1 mentions)

Spelling variants of this product

RPMI-1640 medium (3704 citations) RPMI 1640 (3389 citations) RPMI medium (384 citations) 1640 medium (114 citations)

6 protocols using rpmi 1640 nutrient medium

Cell Culture Conditions for Cancer and Normal Cells

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The human tumor breast adenocarcinoma cells MCF-7 and normal fibroblast cells, MRC-5 were maintained in the Roswell Park Memorial Institute (RPMI) 1640 nutrient medium (Sigma Chemicals Co., USA) supplemented with penicillin (100 IU mL⁻¹), streptomycin (200 μg mL⁻¹), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) (25 mM), l-glutamine (3 mM), and 10% of heat-inactivated fetal bovine serum (FBS). The human colorectal adenocarcinoma cells HT-29 (ATCC, USA) and Caco-2 (DSMZ, Germany) were cultured, respectively, in RPMI 1640 medium (Gibco, UK) supplemented with 10% FBS (Biowest, USA) and Dulbecco's Modified Eagle Medium (DMEM; Gibco, USA) supplemented with 1% non-essential amino acids (NEAA; Biowest, USA), 10% FBS and 1% (v/v) penicillin–streptomycin solution (Gibco, USA).
The cells were routinely maintained as monolayers in 75 cm² culture flasks and incubated at 37 °C in a humidified atmosphere containing 5% CO₂.

Popović B.M., Gligorijević N., Aranđelović S., Macedo A.C., Jurić T., Uka D., Mocko-Blažek K, & Serra A.T. (2023). Cytotoxicity profiling of choline chloride-based natural deep eutectic solvents. RSC Advances, 13(6), 3520-3527.

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Cell Culture Conditions for Comparative Analysis

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Human cervical carcinoma (HeLa), human alveolar basal adenocarcinoma (A549), and normal human fetal lung fibroblast cell line (MRC-5) were maintained as monolayer culture in the Roswell Park Memorial Institute (RPMI) 1640 nutrient medium (Sigma Chemicals Co, USA). RPMI 1640 nutrient medium was prepared in sterile ionized water, supplemented with penicillin (192 IU mL⁻¹), streptomycin (200 μg mL⁻¹), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) (25 mM), L-glutamine (3 mM) and 10 % of heat-inactivated fetal calf serum (FCS; pH 7.2). The cells were grown at 37 °C in 5 % CO₂ and humidified air atmosphere, by twice weekly subculture.

Kuhn P.S., Cremer L., Gavriluta A., Jovanović K.K., Filipović L., Hummer A.A., Büchel G.E., Dojčinović B.P., Meier S.M., Rompel A., Radulović S., Tommasino J.B., Luneau D, & Arion V.B. (2015). Heteropentanuclear Oxalato-Bridged nd–4f (n=4, 5) Metal Complexes with NO Ligand: Synthesis, Crystal Structures, Aqueous Stability and Antiproliferative Activity. Chemistry (Weinheim an Der Bergstrasse, Germany), 21(39), 13703-13713.

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Evaluation of Antioxidant and Antidiabetic Properties

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In this study, absolute ethanol (Sani-Hem doo, Novi Bečej, Serbia), Folin–Ciocalteu’s reagent (Carlo Erba Reagents, Val de Reuil, France), calcium chloride dihydrate, methanol, acetic acid (Zorka Pharm, Šabac, Serbia), gallic acid (purity of 97%) (Merck, Darmstadt, Germany), α-glucosidase, 4-nitrophenyl α-D-glucopyranoside (PNP-G), acarbose, 2,2-diphenyl-1-picrylhydrazyl (DPPH), butylhydroxytoluene (BHT), 3-(4,5-dimethylthiazol-2-yl)-2,5-dyphenyl tetrazolium bromide (MTT), phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO), sodium dodecyl sulfate (SDS), RPMI 1640 nutrient medium, simulated gastric fluid (pH 1.2, SGF), and simulated intestinal fluid (pH 7.4, SIF) without enzyme (Sigma-Aldrich, St. Louis, MO, USA), alginic acid sodium salt (very low viscosity), water (LC-MS grade), acetonitrile (LC-MS grade), formic acid (Thermo Scientific^TM, Waltham, MA, USA), chitosan (molecular weight from 100,000 to 300,000) (Acros Organics, Thermo Fisher Scientific, Geel, Belgium) were used. Other used chemicals were pro analysis quality.

Boškov I.A., Savić I.M., Grozdanić Stanisavljević N.Đ., Kundaković-Vasović T.D., Radović Selgrad J.S, & Savić Gajić I.M. (2024). Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles. Polymers, 16(5), 688.

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Cultivation of Carcinoma and Fibroblast Cells

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Human colon carcinoma cells (LS-174) and normal human lung fibroblasts cells (MRC5), kindly gifted from the Institute of Oncology and Radiology of Serbia, were maintained as monolayer culture in the Roswell Park Memorial Institute (RPMI) 1640 nutrient medium (Sigma Chemicals Co., St. Louis, MO, USA). RPMI 1640 nutrient medium was prepared in sterile deionized water, supplemented with penicillin (100 U/mL), streptomycin (100 mg/mL), 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) (25 mM), L-glutamine (3 mM) and 10% of heat-inactivated fetal calf serum (FCS) (pH 7.2). The cells were grown at 37 °C in 5% CO₂ and humidified air atmosphere.

Đoković J.B., Savić S.M., Mitrović J.R., Nikolic I., Marković B.D., Randjelović D.V., Antic-Stankovic J., Božić D., Cekić N.D., Stevanović V., Batinić B., Aranđelović J., Savić M.M, & Savić S.D. (2021). Curcumin Loaded PEGylated Nanoemulsions Designed for Maintained Antioxidant Effects and Improved Bioavailability: A Pilot Study on Rats. International Journal of Molecular Sciences, 22(15), 7991.

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Culturing Human Cervical Carcinoma Cells

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The human cervical carcinoma (HeLa) cells were maintained as a monolayer culture in the Roswell Park Memorial Institute (RPMI) 1640 nutrient medium (Sigma Chemicals Co, USA). RPMI 1640 nutrient medium was prepared in sterile deionised water, supplemented with penicillin (192 IU/mL), streptomycin (200 µg/mL), 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) (25 mM), L-glutamine (3 mM), and 10% of heat-inactivated fetal calf serum (FCS) (pH 7.2). The HeLa cells were grown at 37 °C in 5% CO 2 in a humidified air atmosphere.

Jovanović K.K., Tanić M., Ivanović I., Gligorijević N., Dojčinović B.P, & Radulović S. (2016). Cell cycle, apoptosis, cellular uptake and whole-transcriptome microarray gene expression analysis of HeLa cells treated with a ruthenium(II)-arene complex with an isoquinoline-3-carboxylic acid ligand. Journal of inorganic biochemistry, 163.

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Cell Culture Protocols for Cancer Research

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Human cervix carcinoma cells (HeLa), human breast cancercells (MDA-MB-231), human colon carcinoma cells (LS-174), lungadenocarcinoma cells (A549), human myelogenous leukemia cells (K562) and human fetal lung fibroblast cells (MRC-5) were main-tained in the Roswell Park Memorial Institute (RPMI) 1640 nutrientmedium (Sigma Chemicals Co., USA). RPMI 1640 nutrient mediumwas prepared in sterile deionized water, supplemented with peni-cillin (192 U/mL), streptomycin (200 μg/mL), 4-(2hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES) (25 mM), L-glutamine (3 mM) and 10% of heat-inactivated fetal calf serum (FCS) (pH 7.2).The cells were grown at 37•C in 5% CO2and humidified air atmo-sphere.

Skorić M., Gligorijević N., Čavić M., Todorović S., Janković R., Ristić M., Misic D, & Radulović S. (2017). Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action. Industrial Crops & Products., 100.

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