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6 protocols using absolute ethyl alcohol

1

Synthesis of Magnetic Nanoparticles

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Platinum acetylacetonate [Pt(acac)2, purity 97%], magnesium acetylacetonate [Mg(acac)2·2H2O, purity 98%], cobalt acetylacetonate [Co(acac)2, purity 97%], oleylamine (OAm, 70%) and oleic acid (OA, 90%) was purchased from Sigma Aldrich. Pentacarbonyl iron [Fe(CO)5 purity 99%], nitric acid(HNO3, concentration 68%), methylbenzene(purity 99%) and absolute ethyl alcohol was purchased from Beijing Chemical Works. All chemicals were used as received.
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2

Fluorescent Silica Nanoparticle Synthesis

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Fluorescein isothiocyanate (FITC, 95%), 3-aminopropyltriethoxysilane (APTES, 98%), tetraethyl orthosilicate (TEOS, 98%), and ammonium hydroxide (NH4OH, 28%−30% as NH3) were purchased from Alfa Aesar Ltd (Shanghai, China). Absolute ethyl alcohol was acquired from the Beijing Chemical Reagent Company (Beijing, China). All materials were used as received and without further purification. Purified water was used throughout.
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3

P2Y Receptor Expression in Cell Lines

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ATP and UTP were purchased from Sigma (St. Louis, MO, USA) and dissolved in ddH2O to a concentration of 100 mM. The antibodies of P2Y1 (H-120), P2Y2 (H-70), P2Y4 (H-60), P2Y6 (H-70), Snail (H-130), E-cadherin (G-10) and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Absolute ethyl alcohol, dimethyl benzene, formaldehyde and hydrogen peroxide were purchased from Beijing Chemical Works (Beijing, China).
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4

Synthesis of Lanthanide-Doped PLGA Nanoparticles

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PLGA (Mw = 1 × 105, LA : GA = 85 : 15) was purchased from Jinan Daigang Biotechnology Co., LTD (China). La2O3 (99%), Tb4O7 (99%) and benzimidazole (Bim) were purchased from Sinopharm Chemical Reagent Co., LTD (China). NaOH, FeCl3·6H2O and FeCl2·4H2O were provided by Xilong Chemical Co., LTD (China). Hydrogen peroxide (H2O2, 30%), hydrochloric acid (HCl), trichloromethane (CHCl3), tetrahydrofuran (THF), dichloromethane (CH2Cl2) and absolute ethylalcohol were provided by Beijing Chemical Works (China). Hexafluoroisopropanol (HFIP) was purchased from Aladdin Industrial Corporation. All reagents involved in this work were of analytical grade and directly used as-received without any further purification.
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5

Fuel Cell Catalyst Preparation

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XC-72 (Vulcan), FeSO4·7H2O (Shanghai Sinopharm Chemical Reagent Co Ltd), N,N-dimethylformamide (Beijing Chemical Works), Nafion (5%) (Sigma) and absolute ethyl alcohol (Beijing Chemical Works) were purchased. All the chemicals were of AR grade and used as received without further purification. Commercial 20 wt% Pt/C electrocatalyst was supplied by Sigma-Aldrich.
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6

Synthesis and Characterization of Iron-Citrate Nanoparticles

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Ferric chloride hexahydrate (FeCl3·6H2O) and trisodium citrate dihydrate were purchased from Tianjin Damao Chemical Institute (Tianjin China). Sodium acetate and absolute ethyl alcohol were obtained from Beijing Chemical Reagent Company (Beijing, China). Poly(ethylene glycol) (PEG6000) and N-Hydroxysuccinimide (NHS) were bought from Sigma Company (USA). N-(3-dimethylamlnopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) was bought from Aladdin Chemistry Co. Ltd. (Shanghai, China). Bovine serum albumin (BSA) was purchased from Celartics biopharma Co. Ltd. 2-Morpholinoethanesulfonic acid (MES) was purchased from TCI Ltd. (Shanghai, China). All the above reagents were of analytical grade.
Sephacryl s-100 High Resolution was purchased by GE Healthcare Institute (Sweden). The phosphate-buffered saline (PBS) buffer was bought from Sangon Biotech (Shanghai, China) Co., Ltd. The BCA protein content kit was purchased from DingGuo Company (Beijing, China). The TIANamp Bacteria DNA Kit was obtained from Tiangen Bio (Beijing, China). Deionized water and S. aureus were from the School of Public Health, Jilin University.
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