A tissue microarray chip containing 28 pairs of human colon cancer tissues and matched adjacent normal colon tissues is purchased from Shanghai Outdo Biotech CO., LTD. (Shanghai, China, HColAde060CS01). The expression of STBD1 is evaluated with the anti-STBD1 antibody (proteintech, 67018-1-Ig) by IHC at a dilution of 1:200. The staining substrate was 3,3′-diaminobenzidine (DAB) and the nuclei is counterstained with hematoxylin. Then the tissue microarray chip is scanned using Pannoramic MIDI (3D HISTECH). The STBD1 expression was performed based on staining intensity by analyzing the IOD of the dark brown color in each image using NIH Image J software.
Tissue microarray chip
Manufactured by Shanghai Outdo Biotech Co.
Sourced in China
Tissue microarray chips are a laboratory tool used to facilitate high-throughput analysis of tissue samples. They consist of small cylindrical tissue samples, or cores, that are extracted from paraffin-embedded tissue blocks and arranged in a grid-like pattern on a microscope slide or membrane. This allows for the simultaneous examination of multiple tissue samples under the same experimental conditions.
Automatically generated - may contain errors
12 protocols using tissue microarray chip
1
Immunohistochemical Evaluation of STBD1
2
Nasopharyngeal Carcinoma Tissue Acquisition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three pairs of NPC tissues and nasopharyngitis tissues were collected by endoscopic biopsy in the Affiliated Hospital of Guangdong Medical University. All tissues were confirmed by pathological examination and stored in liquid nitrogen immediately. A tissue microarray chip containing clinicopathological characteristics of 130 NPC patients was bought from the Shanghai Outdo Biotech Company. The experiment was approved by the ethics committee of the Affiliated Hospital of Guangdong Medical University, and all patients signed the consent for specimen retention. Two external cohorts, GSE102349 and GSE61218, were enrolled in this study, and the transcriptome profiles and clinical data were downloaded from the Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/ ).
3
Ovarian Tumor Tissue Microarray Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue microarray chips consisting of the ovarian tumor tissue specimens (Supplementary Tables S1 , S2 ) were purchased from Outdo Biotech, Ltd. (Shanghai, China). Hematoxylin-eosin (H&E) staining was performed by following the routine method and IHC of TMA chips was performed following standard IHC staining protocol with primary antibodies against DDB1 (1:30,000) and CUL4A (1:5000). The assignment of the positive-staining score was based on the following standard: the percentage of staining-positive cells (A) by the intensity (B: 0, negative; 1, weakly positive; 2, positive; 3, strongly positive). The final score for each case was calculated as A × B. Mann–Whitney U-method was used for statistical analysis.
Tumor xenografts were formalin-fixed, paraffin-embedded, and sectioned according to the standard protocol.79 (link) Slides were incubated with primary antibodies (anti-CUL4A 1:200, anti-DDB1 1:200, anti-DRP1 1:400 and anti-Parkin 1:50) at 4 °C overnight and were subjected with secondary antibody (1:250) for 40 min at 25 °C . Then, the sections were performed with DAB chromogen and counterstained with hematoxylin. All image were visualized using a Leica DM 2000 microscope.
Tumor xenografts were formalin-fixed, paraffin-embedded, and sectioned according to the standard protocol.79 (link) Slides were incubated with primary antibodies (anti-CUL4A 1:200, anti-DDB1 1:200, anti-DRP1 1:400 and anti-Parkin 1:50) at 4 °C overnight and were subjected with secondary antibody (1:250) for 40 min at 25 °C . Then, the sections were performed with DAB chromogen and counterstained with hematoxylin. All image were visualized using a Leica DM 2000 microscope.
4
Tissue Microarray Proteome Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All tissue microarray chips were purchased from Shanghai Outdo Biotech Co. Ltd. Tumor and paracancerous tissues (normal) were freshly excised from a patient with NSCLC undergoing surgery. Tumor tissue and matched paracancerous tissue were homogenized (53 (link)). Briefly, the specimens were cut into 0.5-mm sections before digestion with 0.1% collagenase IV (Gibco, no. 17104019) for 1 hour at 37°C. The cells were then passed through a 70-μm cell strainer (BD, no. 352350) and collected by centrifugation for 15 min at 400g. Plasma membrane proteome extracts were prepared from single-cell suspensions of tissues.
5
Evaluating PD-L1, KLK6, and CSN1S1 in LUSC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue microarray chips containing 90 samples of LUSC and 90 samples of paired normal lung tissue were purchased from Outdo Biotech (Shanghai, China). Immunohistochemistry (IHC) staining was performed as previously described.35 ,36 Microarray chips were stained with PD-L1 (Gene Tech), KLK6 (R&D) and CSN1S1 (Bioss). Quantitative analysis of the staining was independently assessed based on the percentage of positive cells and staining density by two experienced pathologists.37 The final score was determined by adding the staining intensity score and the average proportion of positive cells score.
6
Evaluating LRRC59 Expression in OSCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue microarray chips were purchased from Outdo Biotech Company; they contained 55 samples of OSCC and 5 samples of normal control (Shanghai, China). Taizhou Hospital (Zhejiang, China) provided the samples for this study. Detailed information of clinicopathological features for these patients were displayed in Table S1 .
IHC studies of LRRC59 were performed in tissue microarray as below: following deparaffinization and drying, Rabbit polyclonal LRRC59 antibody was applied to the Tissue microarray chips overnight at 4 °C (dilution 1:500, Abcam) shortly after epitope retrieval, hydrogen peroxide treatment, and blocking of non-specific antigens. A secondary antibody was then applied to the chips, and the signal was detected using a DAB staining kit. All stainings were assessed based on the strength and extensity of positive cells, with the intensity (0 = genitive, 1 = weak, 2 = moderate, and 3 = strong) and extensity (0 = 10% or less of cells stained positive; 1 = 11%–40%; 2 = 41%–70%; 3 = 71% or more) of tumor staining being examined. To obtain the final score, the total extensity score was multiplied by the highest intensity score (maximum value of 9).
IHC studies of LRRC59 were performed in tissue microarray as below: following deparaffinization and drying, Rabbit polyclonal LRRC59 antibody was applied to the Tissue microarray chips overnight at 4 °C (dilution 1:500, Abcam) shortly after epitope retrieval, hydrogen peroxide treatment, and blocking of non-specific antigens. A secondary antibody was then applied to the chips, and the signal was detected using a DAB staining kit. All stainings were assessed based on the strength and extensity of positive cells, with the intensity (0 = genitive, 1 = weak, 2 = moderate, and 3 = strong) and extensity (0 = 10% or less of cells stained positive; 1 = 11%–40%; 2 = 41%–70%; 3 = 71% or more) of tumor staining being examined. To obtain the final score, the total extensity score was multiplied by the highest intensity score (maximum value of 9).
7
Hepatocellular Carcinoma Cell Line and Tissue Sample Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HCC cell lines, including MHCC-LM3, MHCC97H, SK-hep1, BEL7402 and SMMC7721, were from the National Infrastructure of Cell Line Resources of China and were cultured in DMEM or 1640 supplemented with 10% fetal bovine serum (FBS) plus antibiotics at 37 °C in a 5% CO2 atmosphere. A total of 5 paired HCC and adjacent non-tumor tissue samples were obtained from patients with HCC who underwent surgery at the Third Affiliated Hospital, Guangzhou Medical University. All patients were confirmed to have HCC via histopathological evaluations. None of the patients had been treated before surgery. Detailed information pertaining to clinicopathological characteristics was recorded. All tissue samples were rapidly snap-frozen in liquid nitrogen and stored at -196 °C until RNA and protein extraction. Informed consent was obtained from all patients. Our study was approved by the Research Ethics Committee of the Third Affiliated Hospital of Guangzhou Medical University, China. Tissue microarray chips comprising 85 pairs of matched HCC and non-cancerous liver tissue plus 10 HCC tissue samples and their clinicopathological information were purchased from Shanghai OUTDO Biotech Co, Ltd (Shanghai).
8
Profiling Pancreatic Cancer Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue microarray chips containing 99 samples of pancreatic cancer and 71 samples of paired normal pancreatic tissue were purchased from Outdo Biotech (Shanghai, China). The characteristics of the patients are shown in Supplementary File S1. Immunohistochemistry (IHC) staining was performed as previously described [23 (link)]. Microarray chips were stained with anti-CENPF (Abcam), anti-SCEL, anti- GLUT-1, anti-SLC6A14, anti-TMC7 (Thermofisher, Waltham, MA USA), anti-TMPRSS4 and anti- MASpin (Gene Tex, Irvine, CA, USA) antibodies. Control staining with only secondary antibodies was performed to ensure specificity. Rabbit IgG polyclonal-Isotype control (Abcam) was used as the negative control. The score for staining was independently assessed by two experienced pathologists based on the integrated staining intensity and the proportion of positive cells. The final score was determined by adding the staining intensity score and the average proportion of positive cells score; the final score ranged from 0 to 7. For the purpose of further analysis, the samples with a score of 0–3 were defined as low expression, while the samples with scores of 4–7 were defined as high expression.
9
Ovarian Tumor Tissue Microarray Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue microarray chips consisting of ovarian tumor tissue specimens were purchased from Outdo Biotech, Ltd. (Shanghai, China). The specimens were collected from the National Human Genetic Resources Sharing Service Platform (Platform No. 2005DKA21300), with approval from the Institutional Ethics Committee of Outdo Biotech, Ltd. (Approval No. SHYJS-CP-1804010).
The animal experiments were performed in strict accordance with the People’s Republic of China Legislation Regarding the Use and Care of Laboratory Animals. All protocols used in this study were approved by the Institutional Animal Care and Treatment Committee of Sichuan University in China. (Approval No. 20220218005).
The animal experiments were performed in strict accordance with the People’s Republic of China Legislation Regarding the Use and Care of Laboratory Animals. All protocols used in this study were approved by the Institutional Animal Care and Treatment Committee of Sichuan University in China. (Approval No. 20220218005).
10
Gastric Cancer Tissue Immunohistochemistry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Normal GC tissue samples and gastric tumor tissue samples were collected at Clinical Medical College of Jinan University. Tissue sample collection was approved by the Internal Review and Ethics Boards of Jinan University. Tissue microarray chips containing normal gastric tissue samples and GC tumor tissue samples were obtained from Shanghai OUTDO Biotech. Immunohistochemical staining and quantification were undertaken as described previously.21 The immunostaining was blindly scored by pathologists. The immunohistochemical score was calculated as described previously.21 The χ2 test and Pearson's correlation coefficient were used for statistical analysis of the correlation between STUB1 and YAP1 expression.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!