Tannic acid

Manufactured by Maclin Biochemical Technology

Sourced in China

Tannic acid is a naturally occurring polyphenolic compound. It is commonly used as a reagent in various laboratory applications due to its ability to precipitate proteins and bind to metal ions.

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Lab products found in correlation

9 protocols using tannic acid

Tannic Acid Effects on Hyphantria cunea Larvae

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Tannic acid (purity: 98%; CAS: 1401-55-4) was purchased from Shanghai Macklin Biochemical Co., Ltd. The Tannic acid concentration was set at 12.5 mg/g, within the range of the Tannic acid concentration in L. olgensis needles (4.17–72.07 mg/g) [37 ,38 ]. Tannic acid was dissolved with distilled water (250 mg/mL), and then 10 mL of Tannic acid solution was added to 190 mg artificial diets to prepare a diet with a Tannic acid concentration of 12.5 mg/g. In the untreated artificial diets, we added 10 mL of distilled water. Subsequently, the healthy larvae at 3rd instar were divided into two groups: one fed on an untreated artificial diet (control group) and the other on a Tannic acid-treated artificial diet (treatment group). The artificial diet was changed once a day until the larvae pupated. A total of 300 larvae were raised in each group. Of these, 150 larvae were used to calculate mortality, and the remaining larvae were used for subsequent experiments. In the experiment to calculate mortality, we set up three replicates, each of which included 50 H. cunea larvae.

Tan M., Wu H., Yan S, & Jiang D. (2022). Evaluating the Toxic Effects of Tannic Acid Treatment on Hyphantria cunea Larvae. Insects, 13(10), 872.

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Antimicrobial Hydrogel Synthesis

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Polyvinyl alcohol (PVA, polymerization degree 1799, 99% Anhui Wanwei Co., Anhui, China), Calcium chloride (CaCl₂, Aladdin), β-Glycerol phosphate disodium salt (Gp, > 95%, Aladdin), Tannic acid (TA, AR, MACKLIN), Glycerol (Shandong Dexinkang Medical Technology Co.). The deionized water for the experiment was produced by the ultra-pure water machine (UPTA-UV-20, Shanghai Shenfen Analytical Instrument, China) in our laboratory. Strains of E. coli (ATCC 25922) and S. aureus (ATCC 6538) were obtained from Beijing Baocang Biotechnological Company.

Chen K., Liang K., Liu H., Liu R., Liu Y., Zeng S, & Tian Y. (2023). Skin-Inspired Ultra-Tough Supramolecular Multifunctional Hydrogel Electronic Skin for Human–Machine Interaction. Nano-Micro Letters, 15, 102.

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Synthesis and Characterization of MOF-Tannic Acid Nanocomposite

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Ferric chloride hexahydrate (FeCl₃•6H₂O), amino terephthalic acid (BDC-NH₂), and tannic acid (TA) were acquired from Macklin (Shanghai, China). ICG-NHS was purchased from Ruixibio (Xi’an city, China). Potassium bromide (KBr) was purchased from Meryer (Shanghai, China). Methylene blue (MB) was purchased from Rhawn (Rhawn Co., Ltd., Shanghai, China). ROSGreen H₂O₂ probes were purchased from Shanghai Maokang Biotechnology, China. Finally, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) was purchased from Beyotime Institute of Biotechnology (Shanghai, China).

Li H., Zhang Y., Liang L., Song J., Wei Z., Yang S., Ma Y., Chen W.R., Lu C, & Wen L. (2022). Doxorubicin-Loaded Metal-Organic Framework Nanoparticles as Acid-Activatable Hydroxyl Radical Nanogenerators for Enhanced Chemo/Chemodynamic Synergistic Therapy. Materials, 15(3), 1096.

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Surface-modified UF Membrane Fabrication

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The commercial PSF UF membrane (MWCO = 20 000 Da) used as the support membrane was provided by Hangzhou Water Treatment Technology Development Center (China). Taurine (3-amino-1-propanesulfonic, 98%), tannic acid (TA, 98%), polyethyleneimine (PEI, MW = 600, 1000, 1800, 2500, 10 000, 99%), polyethyleneimine (PEI, MW = 70 000, 50%), bovine serum albumin (BSA) and sodium alginate (SA) were purchased from Shanghai Macklin Biochemical Co., Ltd. The inorganic salts (i.e., sodium phosphate tribasic dodecahydrate (Na₃PO₄·12H₂O), sodium chloride (NaCl), magnesium chloride hexahydrate (MgCl₂·6H₂O), anhydrous sodium sulfate (Na₂SO₄) and anhydrous magnesium sulfate (MgSO₄)) were provided from Hangzhou Lanbo Industrial Co., Ltd. Sodium dodecyl sulfate (SDS) and humic acid (HA) were purchased from Aladdin Industrial Corporation. Trimesoyl chloride (TMC, ≥98%) was purchased from Qingdao Benzo Chemical Co., China. Isopar L as an organic solvent was purchased from Exxon Mobil Corporation. Deionized (DI) water obtained by the electrodialysis of reverse osmosis water was used for all experiments. All chemical reagents were of analytical grade and implemented without further purification.

Lin B., Tan H., Liu W., Gao C, & Pan Q. (2020). Preparation of a novel zwitterionic striped surface thin-film composite nanofiltration membrane with excellent salt separation performance and antifouling property. RSC Advances, 10(27), 16168-16178.

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Polyphenol-Iron Dye Solution Formulations

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Moso bamboo (Phyllostachys edulis (Carrière) J. Houz.) sliced veneers were purchased from Yifeng County, Jiangxi Province, China, with dimensions of 350 mm × 130 mm × 0.7 ± 0.1 mm and with a moisture content of about 12 %. Fe (Ⅱ) sulfate heptahydrate (FeSO₄⋅7H₂O, industrial grade, 96 % purity) was obtained from Aladdin Reagent (Shanghai) Co., Ltd. Tannic acid, gallic acid, ferrous chloride tetrahydrate, phenol, methyl blue, and acacia were all AR, 99 % purity, obtained from Macklin Biochemical (Shanghai) Co., Ltd.
Polyphenol-iron dye solutions, formulated using different reagents, were all fixed to 300 ml. The configured dye solution was subjected to a filtration process and stored in a static position protected from light. The types and contents of the components of different formulations of polyphenol-iron dye solution are shown in Table 1.Table 1

Specific ingredients and contents of the five polyphenol-iron solution formulations.

Table 1

Formula Number	Reagent	g/mL
①	TA	0.0117
	GA	0.0038
	FeSO₄·7H₂O	0.015
	10 % Hydrochloric acid	0.0125
	phenol	0.001
	Methyl Bule	0.0035
	Acacia	0.001
②	TA	0.0117
	GA	0.0038
	FeCl₂·4H₂O	0.015
	10 % Hydrochloric acid	0.0125
	phenol	0.001
	Methyl Bule	0.0035
	Acacia	0.001
③	FeSO₄·7H₂O	0.015
	TA	0.0117
	GA	0.0038
④	FeSO₄·7H₂O	0.015
④	TA	0.0117
⑤	FeSO₄·7H₂O	0.015
⑤	GA	0.0038

Zhu M., Liu S., Bai H., Huang Y., Yu Y, & Yu W. (2024). Effect of tannic/gallic acid-iron dyeing treatment on surface color and light fastness of bamboo veneer. Heliyon, 10(2), e24082.

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PVA, Resveratrol, and Tannic Acid Protocol

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Polyvinyl alcohol (PVA, type 1788), resveratrol (RE, 99%) and tannic acid (TA, analytically pure) were purchased from Shanghai Macklin Biochemical Technology Co., Ltd.

Lin Y., Chen J, & Li H. (2021). Outstanding flame retardancy for poly(vinyl alcohol) achieved using a resveratrol/tannic acid complex. RSC Advances, 12(1), 285-296.

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Characterization of Paeoniae Radix

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RPA was purchased from Yonggang Decoction Piece Factory Co., Ltd. (Bozhou, China). The standard substances including paeoniflorin, benzoylpaeoniflorin, gallic acid, tannic acid, 2′-hydroxy-4′-mehoxyacetophenone, and D-(+)-glucose were HPLC grade and bought from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). The water used in this study was deionized and prepared by the PSDK-C system (Beijing, China).

Jin T., Zhou Z., Zhou J., Ouyang W, & Wu Z. (2021). The Potential Effects of Dielectric Barrier Discharge Plasma on the Extraction Efficiency of Bioactive Compounds in Radix Paeoniae Alba. Frontiers in Nutrition, 8, 735742.

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Tannic Acid-Copper Complex Colorimetric Assay

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Tannic acid (TA), Cu(NO₃)₂·3H₂O, ethanol, hydrogen peroxide (30 wt%), 3,3′,5,5′-tetramethylbenzidine (TMB), L-proline, glycine, cysteine, alanine, glutamic acid, NaCl, KCl, MnCl₂·4H₂O, CaCl₂, glucose and L-glutathione reduced were purchased from Macklin Biochemical Co., Ltd. Ammonia solution (25–28 wt%) and formaldehyde (37–40 wt%) were purchased from Tianjin Zhiyuan Chemical Co., Ltd. Pluronic^® F127 was purchased from Sigma-Aldrich. The qualitative filter paper was purchased from Whatman. All the reagents were used without further purification. Deionized water from a Milli-Q Plus system (Millipore) was used in all experiments.

Cheng D., Qin J., Feng Y, & Wei J. (2021). Synthesis of Mesoporous CuO Hollow Sphere Nanozyme for Paper-Based Hydrogen Peroxide Sensor. Biosensors, 11(8), 258.

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Tannic Acid-Induced Cellular Responses

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Tannic acid was purchased from Shanghai Macklin Biochemical Technology Co., Ltd., (Shanghai, China). MTT was obtained from Solarbio (Beijing, China). The bicinchoninic acid (BCA) protein assay kit, LDH cytotoxicity assay kit, superoxide dismutase (SOD) assay kit, catalase (CAT) assay kit, glutathione peroxidase (GSH-PX) assay kit, lipid peroxidation (MDA) assay kit, and ATP assay kit were purchased from Nanjing KeyGen Biotech. Co. Ltd (Nanjing, China). The Annexin V-FITC apoptosis detection kit and mitochondrial membrane potential assay kit were obtained from Nanjing KeyGen Biotech. Co. Ltd. The ROS content assay kit was purchased from Beyotime Biotech. Co. Ltd. (Nantong, China). Rabbit monoclonal antibody of Bcl-2, Bax, PARP-1, caspase-3, caspase-9, cytochrome c, P53, ATM, P-ATM. CDK, P-CDK, cyclin A2, and β-actin, and goat anti-rabbit antibody of IgG-HRP were obtained from Cell Signaling Technology (Boston, MA, USA). Z-LEHD-FMK was purchased from Abcam, Inc. (Cambridge, UK). The 8-OHdG assay kit was purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China).

Wang J., Xiao H., Zhu Y., Liu S., Yuan Z., Wu J, & Wen L. (2019). Tannic Acid Induces the Mitochondrial Pathway of Apoptosis and S Phase Arrest in Porcine Intestinal IPEC-J2 Cells. Toxins, 11(7), 397.

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