Anti calretinin

Dopamine, N^ω-nitro-L-arginine methyl ester hydrochloride (L-NAME), and ascorbic acid were obtained from Sigma-Aldrich Chemicals Co. (USA). Adrenaline, L-741,626, norAdrenaline, ¹H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), and U-46619 were purchased from Cayman Chemical Co. (USA). 6-NitroDopamine and 6-nitroDopamine-d₄ were acquired from Toronto Research Chemicals (Canada). Dopamine‐d₃ hydrochloride, DL‐norAdrenaline‐d₆ hydrochloride, and Adrenaline‐d₆ hydrochloride were acquired from CDN Isotopes (Canada). Strata™-X 33 mm Polymeric Reversed SPE cartridges were bought from Phenomenex (USA) and GIST-HP C₁₈ columns were obtained from Shimadzu (Germany). Sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl₂), magnesium sulfate (MgSO₄), sodium bicarbonate (NaHCO₃), potassium phosphate monobasic (KH₂PO₄), and glucose were acquired from Merck KGaA (Germany). Anti-S-100 (mouse monoclonal antibody, ab4066) and anti-calretinin (rabbit monoclonal antibody; ab92341) were obtained from Abcam.

The following primary antibodies were used: rabbit anti-cleaved caspase-3 (1:500, Cell Signaling Technologies), rabbit polyclonal anti-Calretinin (1:1000, Abcam #ab702, Cambridge, MA), mouse anti-Reelin (1:1000, Millipore #MAB5366, Burlington, MA), rabbit anti-CCK (1:1000, Frontier Institute Co. Ltd #Af350, Hakaido, Japan), rabbit anti-VIP (1:1000, ImmunoStar #20077, Hudson, Wisconsin). Fluorescent Alexa 488-conjugated antibodies (Alexa Fluor-488-conjugated goat anti-mouse IgG, Alexa Fluor-488-conjugated goat anti-rabbit IgG (1:1000, Life Technologies, Carlsbad, CA) were used as secondary antibodies.