Cell spheroids were fixed in 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked in 3% BSA/PBS 10% FBS. The primary antibodies (mouse anti-HIF-2α or mouse anti TG2; 1 : 100) were incubated for 2 h at 4 °C. The fluorescent secondary antibody (rabbit anti-mouse IgG antibody conjugated with FITC; 1 : 100) was added and incubated for 1 h at 4 °C. The cell nuclei were counter-stained with 4′,6-diamidino-2-phenylindole. Fluorescent images were captured using a Leica MB5000B microscope equipped with a DFC480 R2 digital camera and a Leica Application Suite (LAS) software.
Dfc480 r2 digital camera
Manufactured by Leica
The DFC480 R2 is a digital camera designed for microscopy and imaging applications. It features a high-resolution sensor, advanced image processing capabilities, and connectivity options for integration with various imaging systems. The camera is optimized for capturing high-quality, detailed images in a variety of microscopy and scientific imaging settings.
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Lab products found in correlation
3 protocols using dfc480 r2 digital camera
1
Immunofluorescence Analysis of HIF-2α and TG2
2
Quantifying H3K27me3 in Cell Spheroids
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Cell spheroids were let to adhere for 1/2 h to poly-L-lysine coated glass slides and then fixed in 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked in 3% BSA/PBS 10% FBS. The primary antibody (mouse anti-H3K27me3 1:100) was incubated for 2 h at 4°C. The fluorescent secondary antibody (rabbit anti-mouse IgG antibody conjugated with fluorescein isothiocyanate (FITC); 1:100) was incubated for 1 h at 4°C. Fluorescent images were captured using a Leica MB5000B microscope equipped with a DFC480 R2 digital camera and a Leica Application Suite (LAS) software.
3
Immunofluorescent Staining of Cell Spheroids
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Cell spheroids were fixed, permeabilized with 0.5% Triton X-100 in phosphate buffered saline (PBS) and blocked in 3% bovine serum albumin (BSA)/PBS 10% FBS. The primary antibodies (mouse anti-HIF-2α and rabbit anti-KDM6B or ERβ; 1:100) were incubated for 2 h at 4°C. The fluorescent secondary antibodies (goat anti mouse and anti rabbit IgG antibodies conjugated with fluorescein isothiocyanate; 1:100) were added and incubated for 1 h at 4°C. The cell nuclei were counter-stained with 4′,6-diamidino-2-phenylindole. Fluorescent images were captured using a Leica MB5000B microscope equipped with a DFC480 R2 digital camera and a Leica Application Suite software.
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