Ptctp

The following antibodies were used for IF and diluted in TBS supplemented with 0.1% Tween20 and 2% BSA: CyclinA2 (1:400; #sc-751; Santa Cruz), CyclinA2 (1:400; #4656; Cell Signaling), PLK1 (1:400; ab14210; Abcam), pTCTP (1:400; #5251; Cell Signaling), pH2AX (1:800; #2577; Cell Signaling), pCyclinB1 (1:400; #ab55184; Abcam), pLaminA/C (1:400; #2026; Cell Signaling), Alexa Fluor 488-Goat anti-Rabbit (1:2000; #A11008 Life Technologies) and Alexa Fluor 555-Goat anti-Mouse (1: 2000; #A21422 Life Technologies). The following antibodies were used for WB and diluted in TBS supplemented with 0.1% Tween20 and 5% milk powder: CDC6 (1:400; #sc-9964 Santa Cruz), CDT1 (1:400; #sc-28262 Santa Cruz), GAPDH (1:20000; #G8795; Sigma), anti-Rabbit HRP (1:2000; #ab6721; Abcam) and anti-Mouse HRP (1:2000; #ab97023; Abcam).

Lysate extracted from a total of 5 × 10⁵ cells was used to perform western blots. Primary antibodies against TCTP (ab37506, Abcam), pTCTP(#5251, Cell signaling), pEGFR(Life technologies, 44784G), EGFR(#4267, Cell signaling), PLK1 (#4535, Cell signaling), MCL-1 (sc-819, Santa Cruz Biotechnology), CXCL10 (551215, BD Biosicences), β-actin (M177-3, MBL) were used. Western blotting was followed by incubation with the appropriate secondary antibodies conjugated to horseradish peroxidase, anti-rabbit IgG-HRP (Enzo, Cat ADI-SAB-300-J), and anti-mouse IgG-HRP (Enzo, Cat ADI-SAB-100-J). The immunoreactive bands were developed with the chemiluminescence ECL Detection system (GE Healthcare), and signals were detected using a luminescent image analyzer (LAS-4000 Mini).