Under local or general anesthesia, all surgical procedures were performed using the three-port 25-gauge transconjunctival sutureless vitrectomy system (TSV25G; Alcon Constellation; Alcon Laboratories, Fort Worth, TX) with the help of a noncontact wide-angle viewing system (Resight; Carl Zeiss Meditec AG, Jena, Germany). In the inferotemporal, superotemporal, and superonasal quadrants, the 25G trocar together with a microcannula was inserted into the vitreous about 4.0 mm from the corneoscleral limbus. Then, the microcannula was left, but the trocar was withdrawn. The infusion cannula was placed in the inferotemporal quadrant, and other surgical instruments were placed in the superotemporal or superonasal quadrants. At the beginning of PPV, gas perfusion was set to open. Then, undiluted vitreous samples were collected with 5 ml syringes. Immediately, the samples were transferred into microfuge tubes and placed on ice. Each sample was centrifuged at 1007.1 ×g for 10 min at 4 °C. After centrifugation, approximately 0.5–1.2 ml supernatant was obtained and frozen at −80 °C before analysis. Bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) was conducted to determine the total protein concentration in vitreous. Each assay was performed according to the manufacturer’s instructions.
Resight
Manufactured by Zeiss
Sourced in Germany
The Resight is an optical instrument designed for precision imaging and observation tasks. It features high-quality optics and advanced imaging capabilities to provide clear, detailed visual data. The core function of the Resight is to enable accurate and reliable data collection through visual inspection and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Constellation vision system, by Alcon (5 mentions)
Tsv25g, by Alcon (2 mentions)
Bicinchoninic acid protein assay, by Thermo Fisher Scientific (1 mentions)
Ultravit, by Alcon (1 mentions)
Advanced ultravit, by Alcon (1 mentions)
Iolmaster 700, by Zeiss (1 mentions)
Perfluoron, by Alcon (1 mentions)
Grieshaber revolution dsp, by Alcon (1 mentions)
16 protocols using resight
1
Transconjunctival Vitreous Sampling Protocol
2
Ozurdex Implant in Vitrectomy
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Under peribulbar anesthesia, a standard 25-gauge 3-port pars plana vitrectomy was performed on each eye utilizing the Constellation Vision System (Alcon Laboratories Inc., Fort Worth, TX, USA) and a wide-angle noncontact viewing system (Resight®; Carl Zeiss Meditec AG, Jena, Germany). The ILM/ERM complex was dyed with MembraneBlue Dual, (TrypanBlue 0.15% + Brillian Blue G 0.025%, DORC, Zuidland, The Netherlands) and peeled using vitreoretinal forceps until reaching an area two papillary diameters away from the fovea. For eyes with retinal tears or holes, peripheral retinal photocoagulation was performed in addition to a full vitrectomy.
At the end of the surgery, in the first subgroup, following fluid–air exchange, the Ozurdex implant was injected through the inferotemporal sclerotomy after infusion cannula removal and repositioning in the supertemporal sclerotomy. The implant was always visualized in the vitreous cavity thanks to the wide angle viewing system.
In the non-Ozurdex subgroup, a fluid–air exchange was performed to terminate the surgery.
All surgeries were performed by two experienced surgeons (A.B. and S.R.).
At the end of the surgery, in the first subgroup, following fluid–air exchange, the Ozurdex implant was injected through the inferotemporal sclerotomy after infusion cannula removal and repositioning in the supertemporal sclerotomy. The implant was always visualized in the vitreous cavity thanks to the wide angle viewing system.
In the non-Ozurdex subgroup, a fluid–air exchange was performed to terminate the surgery.
All surgeries were performed by two experienced surgeons (A.B. and S.R.).
3
Scleral Buckling for Retinal Detachment
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This study was a retrospective analysis of the medical records of 17 eyes of 16 patients with RRD who had undergone scleral buckling surgery using a non-contact wide-angle viewing system (Resight; Carl Zeiss Meditec AG, Jena, Germany) with a 25-gauge chandelier endoilluminator (Chandelier Lighting System; Alcon Laboratories, Fort Worth, TX, USA). Scleral buckling was performed by a single surgeon, and all patients underwent at least 3 months of postoperative follow-up. The following variables were analyzed: sex, age, preoperative visual acuity, final visual acuity, follow-up period, preoperative and postoperative intraocular pressure (IOP), first and final anatomical success rate, lens status, number and size of retinal breaks, location of retinal breaks, number of quadrants involved, presence or absence of macular involvement, buckling material, postoperative complications, and operative time. For operation time, the first five cases were excluded to account for the procedural learning curve. Operation times were compared with times for conventional scleral buckling procedure cases performed by the same surgeon in the past. The study was approved by our institutional review board (2016-1063) and followed the principles of the Declaration of Helsinki. Also written informed consents were obtained.
4
Peripheral Vitreous Shaving Techniques
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All cases were treated using a 25G-PPV wide-angle noncontact viewing system (Resight; Carl Zeiss Meditec AG, Jena, Germany) for against cause disease with the Constellation Vision System (Alcon Laboratories, Fort Worth, Texas, USA) or an EVA vitrectomy system (DORC, Zuidland, The Netherlands). The peripheral vitreous and retina were detected using iOCT (RESCAN; Carl Zeiss Meditec AG, Jena, Germany), as described previously,7 (link) and recorded for postoperative analysis. The procedures used for the peripheral detection of vitreous detachment via iOCT were as follows. Step 1: performance of a scleral indentation to observe the peripheral part of the retina using a slit lamp microscope illumination. Step 2: activation of iOCT and adjustment of OCT by focusing on the images of the peripheral retina and vitreous. In group A, vitreous shaving was carried out under a slit lamp microscope illumination (Visulux; Zeiss, Oberkochen, Germany) with ocular indentation. In group B, vitreous shaving was performed under a wide-angle noncontact viewing system using chandelier endoillumination (TotalView Chandelier; DORC, Zuidland, The Netherlands) with ocular indentation.
5
Vitrectomy Probe Comparison in Ophthalmology
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The patients were divided into four groups based on four types of vitrectomy probes that are currently used in surgery: 23–7500 (UltraVit, Alcon Laboratories; 23-gauge 7,500 CPM), 23–7500 (UltraVit 25-gauge 7,500 CPM), 25–10K (Advanced UltraVit, Alcon Laboratories; 25-gauge 10,000 CPM), and 27–10K (Advanced UltraVit 27-gauge 10,000 CPM). For each group, data were collected and organized in terms of axial length measured using partial coherence interferometry (IOLMaster 700, Carl Zeiss Meditec), vitrectomy time, number of sutures performed, the occurrence of iatrogenic retinal breaks during surgery, postoperative hypotony (below 6 mmHg), retinal detachment, endophthalmitis, and other complications. The vitrectomy time was defined as the time taken to remove the vitreous within the maximum visible range using a noncontact wide angle viewing system (Resight, Carl Zeiss Meditec) without indentation. The time taken to change instruments and the time spent on other procedures during surgery, such as PVD induction and removing membranes, were excluded from the vitrectomy time.
6
Intraoperative OCT Imaging in Vitreoretinal Surgery
7
Undiluted Vitreous Fluid Harvesting
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Undiluted vitreous fluids were collected from patients before they underwent primary pars plana vitrectomy. Vitreous samples were taken through a three-port 25-gauge transconjunctival suture-less vitrectomy system (TSV25G; Alcon Constellation; Alcon Laboratories, Fort Worth, TX) and were suctioned directly into a 5 ml syringe. Visualization was aided with a non-contact wide-angle viewing system (Resight; Carl Zeiss Meditec AG, Jena, Germany). The undiluted vitreous samples were immediately transferred into microcentrifuge tubes and placed on ice. Each sample was centrifuged at 1360 ×g for 10 min at 4 °C. The supernatant was harvested from these samples and stored at −80 °C before analysis.
8
Vitreous and Plasma Collection Protocol for Wet AMD
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All patients previously diagnosed with Wet AMD were followed and operated by a single vitreoretinal surgeon (F.A. Rezende). Control patients underwent surgical treatment for nonvascular pathology (epiretinal membrane or macular hole) by the same surgeon. In an operating room setting, patients underwent surgery under local retro/peribulbar anesthesia. A 5% povidone-iodine solution was used to clean the periocular skin, and topical instillation into the eye and within the cul-de-sac was left in place for 5 minutes. Three-port25-gauge trans conjunctival pars plana vitrectomy was performed through 25-gauge valved cannulas (Alcon). Under microscope visualization using a wide-angle viewing system (Resight, Zeiss), undiluted vitreous humour was collected with a 25-gauge vitrector. Vitreous humour was aliquoted in sterilized tubes and directly frozen on dry ice. Blood collection had been proceeding before solutes (IV) installation and vitreous humour biopsy. Blood was collected in tube containing 7.2 mg of K2 EDTA tube (purple). To collect plasma, blood was centrifuged for 10 min at 1500 X g RPM. The plasma was transferred in new tubes and immediately frozen at −807°C.
9
Vitreous Sampling for Analysis
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Prior to primary pars plana vitrectomy, the vitreous samples were obtained using a three-port 25-gauge transconjunctival suture-less vitrectomy system (TSV25G; Alcon Constellation; Alcon Laboratories) with the aid of a non-contact wide-angle viewing system (Resight; Carl Zeiss Meditec AG) for visualization. The samples were then suctioned directly into a 5-ml syringe, were immediately transferred into microcentrifuge tubes and kept on ice. Following centrifugation at 1,360 x g for 10 min at 4˚C, the supernatants were collected and stored at -80˚C prior to analysis.
10
Vitreoretinal Surgery with Wide-Angle Viewing
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All the surgical cases were performed by a single surgeon (MK) using a 25-gauge vitrectomy system with a wide-angle viewing system (Resight®, Carl Zeiss Meditec AG, Jena, Germany) or with phacoemulsification and intraocular lens implantation, if necessary. To protect the cornea from drying during the vitreoretinal surgery, viscoelastic material was applied to the corneal surface and then the lens was positioned on the top of the cornea using fingers or a forceps.
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