α smooth muscle actin α sma

Manufactured by Affinity Biosciences

Sourced in United States

α-smooth muscle actin (α-SMA) is a contractile protein found in the cytoskeleton of smooth muscle cells. It plays a key role in maintaining the structural integrity and contractility of these cells.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Ecl reagent, by Thermo Fisher Scientific (1 mentions) Lamp2, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Ripa buffer, by Beyotime (1 mentions) Radioimmunoprecipitation assay lysis buffer, by Solarbio (1 mentions) Phenylmethanesulfonyl fluoride, by Solarbio (1 mentions) Gapdh, by Solarbio (1 mentions) Fibronectin, by Affinity Biosciences (1 mentions) Hrp conjugated secondary antibody, by Solarbio (1 mentions) Pvdf membrane, by Affinity Biosciences (1 mentions) Nlrp3, by Affinity Biosciences (1 mentions) Collagen 1, by Affinity Biosciences (1 mentions) Bca assay kit, by Solarbio (1 mentions) Cleaved caspase 1, by Affinity Biosciences (1 mentions)

3 protocols using α smooth muscle actin α sma

Nanoelectroporation Impacts Tumor Microenvironment

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Sixteen mice were treated with nsPEFs of 30 kV/cm amplitude, 300 ns duration for 400 pulses in a single treatment.
At various intervals after nsPEFs treatment (0, 1, 3, and 14 d), sixteen nude mice previously grafted with human tumor were euthanized by cervical dislocation and the tumors were excised. The harvested tissues were fixed rapidly in 4% paraformaldehyde, embedded in paraffin, and sliced into 5-µm sections. Some slices were stained with hematoxylin and eosin, dehydrated using an ethanol gradient (100%, 90%, and 75%), and deparaffinized with xylene. Some of these slices were immunostained using antibodies against caspase-3 (Biocare Medical, Concord, CA) for the analysis of apoptotic cell death. Ki-67 staining was used as a proliferation marker in malignant tumors. Stroma within the tumor was also detected with α-smooth muscle actin (α-SMA) (Affinity Biosciences, Cincinnati, USA), fibroblast activation protein-α (FAP-α) (Affinity Biosciences, Cincinnati, USA), and hyaluronan-binding protein 1 (HABP1) (Affinity Biosciences, Cincinnati, USA) staining.

Zhao J., Chen S., Zhu L., Zhang L., Liu J., Xu D., Tian G, & Jiang T. (2021). Antitumor Effect and Immune Response of Nanosecond Pulsed Electric Fields in Pancreatic Cancer. Frontiers in Oncology, 10, 621092.

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Western Blot Analysis of Hepatic Stellate Cell Protein Expression

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LX2 cells were treated according to the experimental design. LX2 cell lysates were collected in ice-cold RIPA buffer (Beyotime Institute of Biotechnology). Total protein was quantified using a BCA protein assay kit (Thermo Fisher Scientific, Inc.). Proteins (5 µg) were separated via SDS-PAGE using 10% gels, and electrotransferred to PVDF membranes (MilliporeSigma), which were incubated with anti-collagen-I (COL-I; cat. no. AF7001; 1:1,000; Affinity Biosciences), α-smooth muscle actin (α-SMA; cat. no. 19245; 1:1,000; Cell Signaling Technology, Inc.), LC3 (cat. no. 2775; 1:1,000; Cell Signaling Technology, Inc.), P62 (cat. no. 8025; 1:1,000; Cell Signaling Technology, Inc.), LAMP2 (cat. no. 49067; 1:1,000; Cell Signaling Technology, Inc.) and β-actin (cat. no. 4970; 1:1,000; Cell Signaling Technology, Inc.) primary antibodies at 4°C overnight. Subsequently, membranes were incubated with horseradish peroxidase (HRP)-conjugated anti-Rabbit IgG secondary antibody (cat. no. SA00001-2; 1:2,000; ProteinTech Group, Inc.) for another 2 h at room temperature. All blots were visualized using enhanced chemiluminescence (ECL) reagents (Thermo Fisher Scientific, Inc.), and the scanned band images were semi-quantified using ImageJ software version 1.8 (National Institutes of Health).

Cheng R., Xu H, & Hong Y. (2021). miR221 regulates TGF-β1-induced HSC activation through inhibiting autophagy by directly targeting LAMP2. Molecular Medicine Reports, 24(5), 777.

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Kidney Protein Extraction and Analysis

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Kidney tissues of rats from each group were harvested. Proteins in kidney tissues and cells were extracted using radioimmunoprecipitation assay lysis buffer (Solarbio, Beijing, China) containing 1% phenylmethanesulfonyl fluoride (Solarbio) on ice. After determination of protein concentration with a BCA Assay Kit (Solarbio), the total protein lysates were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto polyvinylidenedifluoride (PVDF) membranes (Millipore Corp, Darmstadt, Germany). Nonspecific binding sites were blocked with 5% skim milk. Then, the PVDF membranes were incubated overnight with the following primary antibodies: fibronectin (1:500; Affinity Biosciences), α-smooth muscle actin (α-SMA) (1:500; Affinity Biosciences), collagen I (1:1,000; Affinity Biosciences), collagen III (1:1,000; Affinity Biosciences), NLRP3 (1:500; Affinity Biosciences), cleaved caspase-1 (1:1,000; Affinity Biosciences), and GAPDH (1:5,000; Solarbio Life Science) at 4°C, followed by incubation at 37°C for 60 min with HRP-conjugated secondary antibodies (1:3,000; Solarbio). The membranes were visualized using an Enhanced Chemiluminescent Substrate Kit (Solarbio).

Zhai J., Li Z., Zhang H., Lu Z., Zhang Y., Li M., Kang J., Yang Z., Ma L., Ma L., Ma Z., Ma X., Zhao F., Ma X., Gao Y., Zhang Y, & Li X. (2023). Coptisine mitigates diabetic nephropathy via repressing the NRLP3 inflammasome. Open Life Sciences, 18(1), 20220568.

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