Log In Sign up
The largest database of trusted experimental protocols

Xs plus kit

Manufactured by Macherey-Nagel
Visit Supplier

The XS plus Kit is a laboratory equipment product from Macherey-Nagel. It is designed for sample preparation and processing tasks in scientific research and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Sybr green system, by Bio-Rad (3 mentions) Iscript reverse transcription supermix, by Bio-Rad (3 mentions) Steponeplus real time pcr system, by Thermo Fisher Scientific (3 mentions) Nucleospin rna xs, by Macherey-Nagel (3 mentions)

Spelling variants of this product

NucleoSpin RNA Plus XS kit (36 citations)

3 protocols using xs plus kit

1

Quantitative RT-PCR Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using Nucleospin RNA XS or XS plus Kit (Macherey-Nagel, Bethlehem, PA). RNA was reverse transcribed to cDNA using iScript™ Reverse Transcription Supermix (Bio-Rad, Hercules, CA). QRT-PCR was performed using SYBR Green system (Bio-Rad) and detected on a StepOne plus Real-Time PCR system (Applied Biosystems, Foster City, CA). Primers for target genes are listed in Supplementary Table 2.
Kwon O.J., Zhang L., Jia D., Zhou Z., Li Z., Haffner M., Lee J.K., True L., Morrissey C, & Xin L. (2020). De novo induction of lineage plasticity from human prostate luminal epithelial cells by activated AKT1 and c-Myc. Oncogene, 39(48), 7142-7151.
+ Open protocol
+ Expand
2

RNA Extraction and qRT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using Nucleospin RNA XS or XS plus Kit (Macherey-Nagel, Bethlehem, Pennsylvania). RNA was reverse transcribed to cDNA using iScript Reverse Transcription Supermix (Bio-Rad, Hercules, California). Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using SYBR Green system (Bio-Rad) and detected on a StepOne plus Real-Time PCR system (Applied Biosystems, Foster City, California). Primers for target genes are listed in Table S4.
Kwon O.J., Choi J.M., Zhang L., Jia D., Li Z., Zhang Y., Jung S.Y., Creighton C.J, & Xin L. (2020). The Sca-1+ and Sca-1− mouse prostatic luminal cell lineages are independently sustained. Stem cells (Dayton, Ohio), 38(11), 1479-1491.
+ Open protocol
+ Expand
3

Quantitative RT-PCR Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted using Nucleospin RNA XS or XS plus Kit (Macherey-Nagel, Bethlehem, PA). RNA was reverse transcribed to cDNA using iScript™ Reverse Transcription Supermix (Bio-Rad, Hercules, CA). QRT-PCR was performed using SYBR Green system (Bio-Rad) and detected on a StepOne plus Real-Time PCR system (Applied Biosystems, Foster City, CA). Primers for target genes are listed in Supplementary Table 2.
Kwon O.J., Zhang L., Jia D., Zhou Z., Li Z., Haffner M., Lee J.K., True L., Morrissey C, & Xin L. (2020). De novo induction of lineage plasticity from human prostate luminal epithelial cells by activated AKT1 and c-Myc. Oncogene, 39(48), 7142-7151.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!