CD11b+ cells that were separated by the MACS magnetic system from either tumor-bearing spleen or intratumoral tissue were adhered to a glass slide by a Cytocentrifuge (Thermo Fisher Scientific K.K., Yokohama, Japan). The cell smears were prepared and stained first with May-Grunwald’s solution (Merck KGaA, Darmstadt, Germany) for 3 min and then with a dilution of May-Grunwald’s solution (1:1 in water) for 1 min. After washing with water, the cell smears were stained for 30 min with × 0.025 diluted Giemsa’s solution (Merck KGaA) diluted with 6.7 mM phosphate buffer, pH 6.4. The smear was then examined under a microscope.
Umemura N., Sugimoto M., Kitoh Y., Saio M, & Sakagami H. (2020). Metabolomic profiling of tumor-infiltrating macrophages during tumor growth. Cancer Immunology, Immunotherapy, 69(11), 2357-2369.
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