Nebnext poly a mrna magnetic isolation module and ultra 2 directional rna library prep kit

Manufactured by Illumina

The NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra™ II Directional RNA Library Prep Kit are laboratory equipment used for the isolation and preparation of messenger RNA (mRNA) and the subsequent construction of directional RNA libraries. The Poly(A) mRNA Magnetic Isolation Module is used to selectively isolate polyadenylated mRNA from total RNA samples, while the Ultra™ II Directional RNA Library Prep Kit is utilized to generate directional RNA libraries from the isolated mRNA for further analysis.

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Lab products found in correlation

Neoprep system, by Illumina (2 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Truseqm stranded mrna library prep protocol, by Illumina (1 mentions) Truseq stranded mrna library prep protocol, by Illumina (1 mentions)

Spelling variants of this product

NEBNext Ultra II Directional RNA Library Prep Kit (244 citations) NEBNext Ultra Directional RNA Library Prep Kit (217 citations) NEBNext Poly(A) mRNA Magnetic Isolation Module (138 citations) Ultra Directional RNA Library Prep Kit (8 citations) Ultra II Directional RNA Library Prep Kit (6 citations) Poly(A) mRNA Magnetic Isolation Module (5 citations) NEBNext Ultra Directional RNA library kit (5 citations) NEBNext Ultra II Directional RNA Library Kit (4 citations) NEBNext UltraTM Directional RNA Library Prep (2 citations) NEBNext® Ultra™ II Directional RNA Library (2 citations)

2 protocols using nebnext poly a mrna magnetic isolation module and ultra 2 directional rna library prep kit

Bulk RNAseq of Muscle Biopsies

Cited 2 times

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Bulk RNAseq was performed on frozen muscle biopsy specimens as previously described^{16 (link)–19 (link)}. Briefly, RNA was extracted with TRIzol (Thermo Fisher Scientific). Libraries were either prepared with the NeoPrep system according to the TruSeqM Stranded mRNA Library Prep protocol (Illumina, San Diego, CA), or with the NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra™ II Directional RNA Library Prep Kit for Illumina (New England BioLabs, ref. #E7490 and #E7760).

Casal-Dominguez M., Pinal-Fernandez I., Pak K., Muñoz-Braceras S., Milisenda J.C., Torres-Ruiz J., Dell′Orso S., Naz F., Gutierrez-Cruz G., Duque-Jaimez Y., Matas-Garcia A., Valls-Roca L., Garrabou G., Trallero-Araguas E., Walitt B., Christopher-Stine L., Lloyd T.E., Paik J.J., Albayda J., Corse A., Grau J.M., Selva-O’Callaghan A, & Mammen A.L. (2023). Coordinated local RNA overexpression of complement induced by interferon gamma in myositis. Scientific Reports, 13, 2038.

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Bulk RNAseq of Muscle Biopsy

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bulk RNAseq was performed on frozen muscle biopsy specimens as previously described.^{6 (link),9 (link)–13 (link)} Briefly, RNA was extracted with TRIzol. Libraries were either prepared with the NeoPrep system according to the TruSeq Stranded mRNA Library Prep protocol (Illumina, San Diego, CA) or with the NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra^™ II Directional RNA Library Prep Kit for Illumina (New England BioLabs, ref. #E7490, and #E7760).

Pinal-Fernandez I., Muñoz-Braceras S., Casal-Dominguez M., Pak K., Torres-Ruiz J., Musai J., Dell’Orso S., Naz F., Islam S., Gutierrez-Cruz G., Cano M.D., Matas-Garcia A., Padrosa J., Tobías-Baraja E., Garrabou G., Aldecoa I., Espinosa G., Simeon-Aznar C.P., Guillen-Del-Castillo A., Gil-Vila A., Trallero-Araguas E., Christopher-Stine L., Lloyd T.E., Liewluck T., Naddaf E., Stenzel W., Greenberg S.A., Grau J.M., Selva-O’Callaghan A., Milisenda J.C, & Mammen A.L. (2024). Pathogenic autoantibody internalization in myositis. medRxiv.

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