Log In Sign up
The largest database of trusted experimental protocols

Uas catalase

Manufactured by Bloomington Drosophila Stock Center
Visit Supplier
Sourced in Japan

The UAS-Catalase is a genetic construct that allows for the overexpression of the catalase enzyme in Drosophila melanogaster. Catalase is an enzyme that plays a crucial role in the breakdown of hydrogen peroxide, a potentially harmful byproduct of cellular metabolism. This construct can be used to study the effects of increased catalase activity on various biological processes in the fruit fly model organism.

Automatically generated - may contain errors

Lab products found in correlation

Uas sod1, by Bloomington Drosophila Stock Center (4 mentions) Uas sod2, by Bloomington Drosophila Stock Center (3 mentions) Uas duox rnai, by Bloomington Drosophila Stock Center (2 mentions) Tubulin gal80ts, by Bloomington Drosophila Stock Center (2 mentions) Tubulin gal4, by Bloomington Drosophila Stock Center (2 mentions) Uas p38b, by Bloomington Drosophila Stock Center (2 mentions) Uas dmkk3, by Bloomington Drosophila Stock Center (2 mentions) Uas coracle, by Bloomington Drosophila Stock Center (2 mentions) Armadillo gal4 arm gal4, by Bloomington Drosophila Stock Center (2 mentions) Tub gal80ts, by Bloomington Drosophila Stock Center (1 mentions) Uas dcr 2, by Bloomington Drosophila Stock Center (1 mentions) Canton s, by Bloomington Drosophila Stock Center (1 mentions) Elav gal4, by Bloomington Drosophila Stock Center (1 mentions) W1118, by Bloomington Drosophila Stock Center (1 mentions) Th gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas lacz, by Bloomington Drosophila Stock Center (1 mentions) Engrailed en gal4, by Bloomington Drosophila Stock Center (1 mentions) Ms1096 gal4, by Bloomington Drosophila Stock Center (1 mentions) Rad21 rnai, by Bloomington Drosophila Stock Center (1 mentions) Uas p35, by Bloomington Drosophila Stock Center (1 mentions)

7 protocols using uas catalase

1

Drosophila Genetic Strains for Research

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
w1118 or genetic background strains were used as control lines in this study. Fly strains used include: Canton-S (Bloomington Drosophila Stock Center, Stock 64349), w1118, SK-/- (kindly provided by Dr. Patrick Dolph) [44 (link)], Df(Shaker) (kindly provided by Dr. Kyunghee Koh) [45 (link)], UAS-DNKv4 [40 (link)], UAS-Kv4/Shal [43 (link)], UAS-Kv1/Shaker (kindly provided by Dr. William Joiner), and UAS-SOD1, UAS-SOD2, UAS-Catalase, UAS-NOX-RNAi and UAS-DUOX-RNAi (all kindly provided by Dr. Matthias Landgraf), UAS-GFP-Kv4/Shal [46 (link)], and elav-GAL4, tub-GAL80ts, UAS-Dcr2 (all obtained from the Bloomington Drosophila Stock Center).
Vallejos M.J., Eadaim A., Hahm E.T, & Tsunoda S. (2021). Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species. PLoS ONE, 16(12), e0261087.
+ Open protocol
+ Expand
2

Drosophila Genetic Toolkit for Circadian Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Flies were reared in 12-h LD cycles in a humidified chamber at 25 °C on a standard corn meal medium. Clk856-GAL438 (link) was a gift from Ralf Stanewsky. The following lines were previously described: HL9-GAL467 (link), R58E02-GAL16 (link), per01,w68 (link), w;tim0169 (link)Pdf-GAL470 (link), Clk1982-GAL438 (link), DvPdf-GAL441 (link)UAS-per1671 (link), and UAS-Shibirets72 (link). NP6510-GAL4 (113956) was obtained from the Kyoto Stock Center. The following lines were obtained from the Bloomington Stock Center: TH-GAL4 (Stock number 8848), R48B04-GAL4 (50347), 0104-GAL4 (62639), MB312B (68314), MB043B (68304), MB299B (68310), MB441B (68251), MB315C (68316), UAS- GCaMP7s (79032), UAS-Kir2.1 (6596), UAS-Catalase (24621), w1118 (5905), Canton-S (64349), UAS-myrGFP, QUAS-mtdTomato(3xHA), and trans-Tango (77124).
Majcin Dorcikova M., Duret L.C., Pottié E, & Nagoshi E. (2023). Circadian clock disruption promotes the degeneration of dopaminergic neurons in male Drosophila. Nature Communications, 14, 5908.
+ Open protocol
+ Expand
3

Genetic Manipulation of Scribble-Ras Oncogenic Axis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The scrib allele used is scrib2 (also known as scrib673) (Bilder and Perrimon, 2000 (link)). The recombinant UAS-RasV12 FRT82B scrib2 (Chen et al., 2012 (link); Pérez et al., 2015 (link)) line was a kind gift of Madhuri Kango-Singh (U Dayton, OH, USA). The MARCM system (Lee and Luo, 1999 (link)) with ey-FLP (Newsome et al., 2000 (link)) was used to generate mosaics of eye/antennal imaginal discs and experimental clones were marked by GFP. In (Figure 1—figure supplement 1), we used a modified MARCM system that marks clones with myrRFP (Chabu and Xu, 2014 (link)). The wt control line (FRT +) is FRT82B (Xu and Rubin, 1993 (link)).
The following transgenes are all inserted on chromosome 2 and were crossed into the scrib−/−RasV12 background for analysis: UAS-lacZ (Bloomington, BL3955), UAS-Duox RNAi and UAS-hCatS (a kind gift of Won-Jae Lee) (Ha et al., 2005a (link); Ha et al., 2005b2005 (link)), UAS-Catalase (BL24621), UAS-SOD1 (BL24754), UAS-SOD2 (BL24494), UAS-p35 (BL5072), UAS-dronc RNAi and UAS-drICE RNAi (a kind gift of Pascal Meier) (Leulier et al., 2006 (link)). UAS-JNKDN (aka UAS-bskDN) (BL6409) is an insertion on X chromosome. Crosses were incubated at either 22° or 25°C.
Pérez E., Lindblad J.L, & Bergmann A. (2017). Tumor-promoting function of apoptotic caspases by an amplification loop involving ROS, macrophages and JNK in Drosophila. eLife, 6, e26747.
+ Open protocol
+ Expand
4

Drosophila Rearing and Fly Strain Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Files were raised on a standard Drosophila diet (4% cornmeal, 6% baker’s yeast (Saf yeast), 6% glucose, and 0.8% agar with 0.3% propionic acid and 0.15% nipagin) at 25°C unless otherwise indicated. For the experiments shown in Figs 1C, 4A–F, 7B–7E, and S6A–S6C, flies were raised at 25°C on an antibiotic-supplemented diet (the standard diet with 0.21% nipagin, 200 μg/mL rifampicin, 50 μg/mL tetracycline, 500 μg/mL ampicillin), by referring to a previous study [11 (link)]. The wiso31 strain developed in a previous study was used as the wild-type strain [69 (link)]. Additionally, the following fly strains were used in this study: WP-QF2, QUAS-Darksh on the 2nd chr., and QUAS-Darksh on the 3rd chr. (generated in our laboratory in previous studies [11 (link),70 (link)]), SPESK6 (kindly provided by Dr. M. Yamamoto-Hino and Dr. S. Goto), MP1SK6, pshSK1, HayanSK3, Hayan-pshDef, and modSP1 (kindly provided by Dr. B. Lemaitre), Tub-Gal4 (DGRC 108069, obtained from Kyoto Drosophila Stock Center, Japan), UAS-Catalase (BL 24621, obtained from Bloomington Drosophila Stock Center, U.S.A.), Lpp-Gal4 (kindly provided by Dr. M. Brankatschk and Dr. S. Eaton, [71 (link)]), UAS-LacZ (DGRC 106500, obtained from Kyoto Drosophila Stock Center, Japan), UAS-persephone (kindly provided by Dr. JM. Reichhart, [31 (link)]), UAS-Hayan and UAS-Hayan mutant (kindly provided by Dr. WJ. Lee, [50 (link)]).
Nakano S., Kashio S., Nishimura K., Takeishi A., Kosakamoto H., Obata F., Kuranaga E., Chihara T., Yamauchi Y., Isobe T, & Miura M. (2023). Damage sensing mediated by serine proteases Hayan and Persephone for Toll pathway activation in apoptosis-deficient flies. PLOS Genetics, 19(6), e1010761.
+ Open protocol
+ Expand
5

Drosophila Genetic Knockdowns for Cellular Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The fly stocks used in this paper are as follows: mad2-RNAi (VDRC 47918), Rad21-RNAi (Bloomington #36786), Eiger (TNFα)-RNAi (VDRC108814), UAS-catalase (Bloomington #24621), HMGB1-RNAi (Bloomington #31960), Drosomycin-GFP [24 (link)], UAS-p35 (Bloomington #5073).
Driver stocks: daughterless (da)-Gal4 for ubiquitous expression, engrailed (en)-Gal4 for gene expression in the posterior region of wing discs and MS1096-Gal4 for wing pouch expression, all from Bloomington Drosophila stock centre.
Liu D., Shaukat Z., Saint R.B, & Gregory S.L. (2015). Chromosomal instability triggers cell death via local signalling through the innate immune receptor Toll. Oncotarget, 6(36), 38552-38565.
+ Open protocol
+ Expand
6

Drosophila Genetic Manipulation Toolkit

Check if the same lab product or an alternative is used in the 5 most similar protocols
UAS-catalase, UAS-SOD1, UAS-SOD2, UAS-p38b+, UAS-DMKK3+, UAS-coracle+, Tubulin-GAL4, Armadillo-GAL4 (Arm-GAL4), and Tubulin-Gal80ts were from the Bloomington Stock Center. UAS-catalaseRNAi, UAS-SOD1RNAi, UAS-SOD2RNAi, UAS-DMKK3RNAi, and UAS-coracleRNAi were from the Vienna Drosophila RNAi Center. D-p38a13 and D-p38b156A were as previously described (Chen et al., 2010 (link)). UAS-p38bDN (Adachi-Yamada et al., 1999 (link)) was a kind gift from T. Adachi-Yamada at Kobe University, Japan. Dot-GAL4 (insertion 11C (c2)) (Kimbrell et al., 2002 (link)) was previously generated in D.A. Kimbrell’s laboratory at the University of California, Davis. GMH5 was as previously described by Wessells et al. (2004) (link). Hand-GAL4 was a kind gift from A. Paululat (University of Osnabruek, Germany).
Lim H.Y., Wang W., Chen J., Ocorr K, & Bodmer R. (2014). ROS Regulate Cardiac Function via a Distinct Paracrine Mechanism. Cell reports, 7(1), 35-44.
+ Open protocol
+ Expand
7

Drosophila Genetic Manipulation Toolkit

Check if the same lab product or an alternative is used in the 5 most similar protocols
UAS-catalase, UAS-SOD1, UAS-SOD2, UAS-p38b+, UAS-DMKK3+, UAS-coracle+, Tubulin-GAL4, Armadillo-GAL4 (Arm-GAL4), and Tubulin-Gal80ts were from the Bloomington Stock Center. UAS-catalaseRNAi, UAS-SOD1RNAi, UAS-SOD2RNAi, UAS-DMKK3RNAi, and UAS-coracleRNAi were from the Vienna Drosophila RNAi Center. D-p38a13 and D-p38b156A were as previously described (Chen et al., 2010 (link)). UAS-p38bDN (Adachi-Yamada et al., 1999 (link)) was a kind gift from T. Adachi-Yamada at Kobe University, Japan. Dot-GAL4 (insertion 11C (c2)) (Kimbrell et al., 2002 (link)) was previously generated in D.A. Kimbrell’s laboratory at the University of California, Davis. GMH5 was as previously described by Wessells et al. (2004) (link). Hand-GAL4 was a kind gift from A. Paululat (University of Osnabruek, Germany).
Lim H.Y., Wang W., Chen J., Ocorr K, & Bodmer R. (2014). ROS Regulate Cardiac Function via a Distinct Paracrine Mechanism. Cell reports, 7(1), 35-44.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!