LaCl3 (purity >99.99%) was purchased from Aladdin Bio-Chem Technology Co. Ltd (Shanghai, China). FM4-64 was purchased from Thermo Fisher Scientific Co. Ltd (Shanghai, China). DPI, TyrA23, Murashige and Skoog medium (MS), methyl 1-(butylcarbamoyl)-2-benzimidazolecarbamate (Benomyl), 6-benzylaminopurine, indole acetic acid (IAA), and JA were purchased from Sigma-Aldrich Co. Ltd (Shanghai, China). Flg22 was purchased from MedChemExpress Co. Ltd (Shanghai, China). Anti-plant actin mouse monoclonal antibody (A01050) was purchased from Abbkine Scientific Co. Ltd (Wuhan, China). Goat anti-mouse IgG H&L [horseradish peroxidase (HRP)] (ab205719) and HRP anti-GFP antibody (ab6663) were purchased from Abcam Co. Ltd (Shanghai, China). Other chemicals used in this study were analytical reagents and were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).
Flg22
Manufactured by MedChemExpress
Sourced in China, United States
Flg22 is a synthetic peptide that corresponds to the highly conserved 22-amino acid sequence (flg22) from the flagellin protein of bacterial flagella. It serves as a microbe-associated molecular pattern (MAMP) that is recognized by plant pattern recognition receptors (PRRs) to initiate innate immune responses.
Automatically generated - may contain errors
Lab products found in correlation
Fm4 64, by Thermo Fisher Scientific (3 mentions)
Tyra23, by Merck Group (1 mentions)
Methyl 1 butylcarbamoyl 2 benzimidazolecarbamate benomyl, by Merck Group (1 mentions)
Indole acetic acid iaa, by Merck Group (1 mentions)
Murashige and skoog ms medium, by Merck Group (1 mentions)
6 benzylaminopurine, by Merck Group (1 mentions)
2 protocols using flg22
1
Molecular Tools for Plant Signaling Analysis
2
Inducible Rice Defense Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Defence‐related gene expression was detected as previously described with minor modification (Wang et al., 2019 (link)). Two‐week‐old leaves of rice seedlings were cut into 2 mm strips,; then, samples were placed in a 2‐mL microcentrifuge tube. The samples were infiltrated with 1 mL sterilized water in 2‐mL microcentrifuge tube overnight in darkness to recover from wounding stress. On the next day, sterilized water was replaced with 10 μm flg22 (MedChemExpress, Wilkinson way, Princeton, USA, Cat. no. 304642‐91‐9) or water as control. After flg22 treatment for 0 and 30 min, the samples were collected and quick‐frozen in liquid nitrogen and stored at −80 °C for RNA extraction and RT‐qPCR assays. Three leaves from different seedlings were collected as one biological replicate, and three biological replicates were used for each treatment. Two technical repeats were performed for RT‐qPCR in gene transcript analysis. The primer sequences for RT‐qPCR assays are listed in Table S1 . After exogenous flg22 treatment for 0, 5, 10, 20 and 30 min, samples were also collected, quick‐frozen in liquid nitrogen and stored at −80 °C for Western blot assays.
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