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3 protocols using enzyme linked immunosorbent assay elisa kits for il 6

1

Investigating Inflammatory Pathways in Cells

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Cell culture reagents including fetal bovine serum (FBS) and RPMI-1640 medium were bought from Gibco BRL Co. (Grand Island, NY, USA). Primary antibodies against p65, COX-2, iNOS, HO-1, PCNA, and β-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse, anti-rabbit, and anti-goat secondary antibodies were bought from Millipore (Billerica, MA, USA). The enzyme-linked immunosorbent assay (ELISA) kits for IL-6, TNF-α, and PGE2 were bought from R&D Systems (Minneapolis, MN, USA). All other chemicals were bought from Sigma-Aldrich (St. Louis, MO, USA).
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2

Klotho and Inflammation Signaling

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Antibodies against ICAM-1, VCAM-1 and GAPDH were purchased from Santa Cruz Biotechnology, Inc (Dallas, TX). Antibody against Klotho was purchased from Abcam Inc. (Cambridge, MA). Recombinant Klotho protein, recombinant IL-37 protein, and Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 were purchased from the R&D System (Minneapolis, MN). ELISA kit for Klotho was purchased from MyBioSource (San Diego, CA). Lipopolysaccharide (LPS, Escherichia coli 0111:B4) and all other chemicals and reagents were purchased from Sigma-Aldrich Chemical (St Louis, MO).
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3

Paeonol Regulates Cardiac Hypertrophy

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Paeonol was purchased from Sigma-Aldrich (St. Louis, MO). Antibodies against to ANP, BNP, α-MHC, collagen I (Col I), connective tissue growth factor (CTGF), fibronectin-1, AKT, p-AKT, ERK1/2, p-ERK1/2, JNK, p-JNK and β-actin were purchased from Cell Signaling Technology (Boston, MA). Enzyme-linked immunosorbent assay (ELISA) kits for IL-6, TNF-α and ANP were purchased from R&D Systems Inc. (Minneapolis, MN).
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