Ab226942

Manufactured by Abcam

Sourced in United Kingdom, China

Ab226942 is a lab equipment product offered by Abcam. It is a tool designed for laboratory use, but no further details about its core function can be provided in an unbiased and factual manner without risk of extrapolation.

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Lab products found in correlation

3 protocols using ab226942

Western Blot Protein Analysis Protocol

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RIPA and BCA assay (Invitrogen) were applied for protein extraction and quantification, respectively. After denaturation, proteins were separated using 10% SDS-PAGE gels. After gel transfer (PVDF membranes) and blocking, incubation with primary and secondary antibodies was performed. Primary antibodies were rabbit anti-GAPDH (1:1800, ab22555, Abcam) and-STAT3 (1:1800, ab226942, Abcam). of the secondary antibody was goat HRP (IgG) (1:2000; ab6721; Abcam). Signals were developed using ECL (Thermo Fisher Scientific). Data was analyzed using Image J v1.46 softwares.

Liu W., Liang F., Yang G, & Xian L. (2021). LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer. BMC Pulmonary Medicine, 21, 50.

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Evaluating EMT Pathway Markers

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The human p-CMV-ICAM-1 vector was purchased from sinobiological, pc-DNA3-SRC vector was purchased from Addgene, and p-CMV6-c-MET vector was purchased from origene. HGF (100-39H) was purchased from Peprotech. SU11274 (c-MET inhibitor), WP1066 (573097), and PP2 (529573) were obtained from Calbiochem. Antibodies specific for CDH2 (#610920) was purchased from BD Biosciences (San Jose, CA, USA). Antibodies specific for Snail(#3879), Slug (#9585), TWIST (#46702),SRC (#2108), p-SRC (#2105), MET (#3127), p-MET (#3077), and HA (#2367) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies specific for Vim (ab137321), CDH1 (ab1416), PDGFB (ab23914), VEGFA (ab46154), FGF2 (ab92337), STAT3 (ab226942), p-STAT3 (ab32143), ZEB1 (ab223688) and ICAM-1 (ab2213) was obtained from Abcam (Cambridge, UK). The monoclonal antibodies specific for β-Actin (SC-47778) was purchased from Santa Cruz. The monoclonal antibodies specific for FLAG (F-3165) was purchased from Sigma. The polyclonal antibodies specific for p-ICAM-1 (CSB-PA000547) was purchased from CUSABIO TECHNOLOGY.

Lim E.J., Kang J.H., Kim Y.J., Kim S, & Lee S.J. (2022). ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer. Cell Death & Disease, 13(4), 417.

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Western Blot Analysis of Protein Expression

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Cells transfected with designated plasmids were lysated in 1×NP40 Lysis buffer containing the complete protease inhibitor (Roche,USA). Then the lysates were centrifuged at 4 o C, 15000g for 15 min. The supernatants were used for western blotting analysis. Proteins (ranging from 20 to 50 µg) from each sample were electrophoresed on a 10% Bis-Tris Criterion XT Precast gel (Bio-Rad, U.S.A), then blotted on 0.2 nitrocellulose membrane using a transfer system (Bio-Rad). The protein blot was blocked by exposure to 5% non-fat milk TBST solution at room temperature for 1 hour. Nitrocellulose lters were then incubated overnight at 4°C with the primary antibodies: anti-STAT3 (Ab226942,abcam), anti-IFI6 [G1P3 Antibody (H-84)] (IC162417; X-Y biotechnology, China), anti-Flag (SAB4200071,Sigma), Anti-H2AFX biovision ,anti-CTNNBL1(ab95170) and secondary antibodies. The GAPDH (ab8245,abcam) was used as loading control.

Deng F., Huang S., & Xiao X. (2021). R354X Mutation in PRPF31 Affects Its Capacity in Inflammation Response and Splicing.

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