N n n n tetramethylethylenediamine temed

Monomeric DMAPAA-Q and DMAPAA were obtained from KJ Chemicals Corporation (Tokyo, Japan). Potassium dichromate (K₂Cr₂O₇) and N,N,N′,N′-tetramethylethylenediamine (TEMED) were obtained from Nacalai Tesque, Inc. (Kyoto, Japan). N,N′-methylenebisacrylamide (MBAA) and ammonium peroxodisulfate (APS) were obtained from Sigma Aldrich Co. (St. Louis, MO, USA). All reagents were used as received. Aqueous solutions were prepared using distilled water. PK208 and PA312 were purchased from Mitsubishi Chemical Corporation (Tokyo, Japan). Organic acids were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). A. woodii is a type strain DSM 1030.

N,N-dimethylamino propylacrylamide monomer (DMAPAAQ) was obtained by KJ Chemicals Co., Tokyo, Japan. Potassium dichromate (K₂Cr₂O₇) and N,N,N’,N’- tetramethylethylenediamine (TEMED) were obtained from Nacalai Tesque, Inc., Kyoto, Japan, N,N’-methylenebisacrylamide (MBAA), ammoniumperoxodisulfate (APS), and potassium permanganate (KMnO₄) were obtained from Sigma Aldrich Co., St. Louis, MO, USA. All the reagents were reagent grade and used as received. Aqueous solutions were prepared using distilled water (DW).

Acrylamide (AAm), CDCl₃, and D₂O were purchased from Wako Pure Chemical Industries, Ltd. βCD was obtained from Junsei Chemical Co., Ltd. Triethylamine (Et₃N), sodium hydroxide, hydrochloric acid, ammonium peroxodisulfate (APS), N,N,N′,N′–tetramethylethylenediamine (TEMED), and N,N′–methylenebis(acrylamide) (MBAAm) were purchased from Nacalai Tesque Inc. Acryloyl chloride and vinyltrimethoxysilane were purchased from Tokyo Chemical Industry Co., Ltd. DMSO–d₆ was obtained from Merck & Co., Inc. RPMI-1640 medium, fetal bovine serum, penicillin and streptomycin were purchased from Sigma-Aldrich, Co. LifeAct-TagGFP2 and the Torpedo® lipofection reagent were purchased from ibidi GmbH. A highly porous synthetic resin (DIAION HP–20) used for column chromatography was purchased from Mitsubishi Chemical Co., Ltd. Water used for the preparation of the aqueous solutions was purified with a Millipore Integral MT system. Other reagents were used without further purification.