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Anti cd25 clone pc61

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Anti-CD25 (clone PC61) is a monoclonal antibody that targets the CD25 antigen, also known as the Interleukin-2 Receptor Alpha Chain (IL-2Rα). This antibody is commonly used in research applications to identify and study cells expressing CD25, such as activated T cells and regulatory T cells (Tregs).

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Lab products found in correlation

Anti cd11b clone m1 70, by BD (3 mentions) Anti cd45.2 clone 104, by Thermo Fisher Scientific (2 mentions) Anti cd8 clone 53 6, by Thermo Fisher Scientific (2 mentions) Anti cd4 clone gk1, by Thermo Fisher Scientific (2 mentions) Anti cd90 2 clone 53 2, by BD (2 mentions) Anti cd3 clone 2c11, by Thermo Fisher Scientific (2 mentions) Anti cd45r b220 clone ra3 6b2, by BioLegend (2 mentions) Anti cd2 clone rm2 5, by BD (2 mentions) Anti siglecf clone e50 2440, by BD (2 mentions) Anti gr 1 clone rb6 8c5, by BD (2 mentions) Anti tcrβ clone h57 597, by BioLegend (2 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Anti cd11c clone n418, by BioLegend (2 mentions) Anti cd4 rm4 5, by BD (1 mentions) Pe conjugated anti mouse cd8a clone 53 6.7, by BD (1 mentions) Cellquest, by BD (1 mentions) Anti foxp3 clone fjk 16s, by Thermo Fisher Scientific (1 mentions) Anti f4 80 clone bm8, by Thermo Fisher Scientific (1 mentions) Anti cd11c clone hl3, by BD (1 mentions) Anti ly6c clone al 21, by BD (1 mentions)

4 protocols using anti cd25 clone pc61

1

Murine Lymphocyte Phenotyping by FACS

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Anti-mouse antibodies for FACS were: PE-conjugated anti-mouse CD8a (clone 53-6.7; BD Pharmingen), AF700-conjugated anti-mouse CD44 (IM7; BD Pharmingen), e450-conjugated anti-mouse FoxP3 (FJK-16s, eBioscience), anti-CD3e (500A2; Biolegend), anti-CD8a (5H10; Caltag), anti-CD4 (RM4-5; BD Pharmingen), V450-conjugated CD45.1 (A20; BD Biosciences), PE-Cy7 conjugated anti-CD45.2 (104; BD Biosciences) and anti-CD25 (Clone PC61; BD Pharmingen). Functional grade anti-CD3 (17A2) and anti-CD28 (37.51) were purchased from eBioscience. For FACS cells were blocked with anti-mouse FcgRIII/IIR (BD Pharmingen) for 10 m and then stained for 45 m on ice with fluorophore-conjugated antibody. Stained cells were washed, fixed with 3% paraformaldehyde and analyzed on LSRII instrument with CellQuest (BD Biosciences) software. Data analyses were performed with FlowJo software (version 8.73; Tree Star).
Buchwald Z.S., Yang C., Nellore S., Shashkova E.V., Davis J.L., Cline A., Ko J., Novack D.V., DiPaolo R, & Aurora R. (2015). A Bone Anabolic Effect of RANKL in a Murine Model of Osteoporosis mediated through FoxP3+ CD8 T-cells. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(8), 1508-1522.
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2

Multiparametric Flow Cytometry Analysis

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Flow cytometry analysis was performed by Attune Acoustic Focusing Cytometer (Life Technologies) using FlowJo software (Tree Star). For Intracellular cytokine staining, cells were stimulated with 20 ng/mL phorbol 12‐myristate 13‐acetate (Sigma) and 1 mmol/L ionomycin (Sigma) for 5 hour in the presence of a GolgiStop (BD Bioscience). The antibodies used were as follows; anti‐CD16/CD32 (clone 2.4G2; BD Bioscience), anti‐CD4 (clone H129.19; BD Bioscience), anti‐CD25 (clone PC61; BD Bioscience), anti‐CD103 (clone M290; BD Bioscience), anti‐GITR (clone DTA1; BD Bioscience), anti‐CTLA‐4 (clone UC10; BD Bioscience), anti‐Foxp3 (clone FJK‐16s; eBioscience), anti‐CD11c (clone HL3; BD Bioscience), anti‐CD80 (clone 16‐10A1; BD Bioscience), anti‐CD86 (clone GL1; BD Bioscience), anti‐CD49b (clone HMa2; BD Bioscience), anti‐LAG3 (clone C9B7W; BD Bioscience), anti‐CD11b (clone M1/70; BD Bioscience), anti‐Ly6C (clone AL‐21; BD Bioscience), anti‐CD115 (clone AFS98; eBioscience), anti‐F4/80 (clone BM8; eBioscience), anti‐CD206 (clone C068C2; BioLegend), anti‐IFNγ (clone XMG1.2; eBioscience), anti‐IL‐4 (clone BVD4‐1D11; eBioscience), anti‐IL‐10 (clone JES5‐16E3; eBioscience) and isotype‐matched control antibodies.
Kasahara K., Sasaki N., Yamashita T., Kita T., Yodoi K., Sasaki Y., Takeda M, & Hirata K. (2014). CD3 Antibody and IL‐2 Complex Combination Therapy Inhibits Atherosclerosis by Augmenting a Regulatory Immune Response. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 3(2), e000719.
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3

Identification and Quantification of ILC2 in BALF

Cited 1 time
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Cells were analyzed by flow cytometry following staining with live/dead aqua dyes (Live/Dead fixable aqua dead cell stain kit, Invitrogen, 1/1000) and antibodies specific for the following markers: anti-TCRβ (clone H57-597, 5.0 μg/ml) from BioLegend; anti-CD45R/B220 (clone RA3-6B2, 1.0 μg/ml), anti-CD2 (clone RM2-5, 0.8 μg/ml), anti-CD25 (clone PC61, 1.0 μg/ml), anti-CD90.2 (clone 53-2.1, 2.5 μg/ml), and anti-CD11b (clone M1/70, 0.3 μg/ml) antibodies from BD Pharmingen; anti-CD45.2 (clone 104, 0.5 μg/ml) from eBioscience. ILC2 were gated as CD45+, CD2− TCRb− B220− CD11b−, CD90+ and CD25+ cells in a similar manner to that described by Roediger et al.10 (link). Bronchoalveolar lavage fluid (BALF) cells were additionally labeled using anti-Siglec F (clone E50-2440, 2.0 μg/ml), from BD Pharmingen; anti-Gr-1 (clone RB6-8C5, 0.4 μg/ml), anti-CD8 (clone 53-6.7, 5.0 μg/ml), anti-CD4 (clone GK1.5, 1.0 μg/ml), anti-CD3 (clone 2C11, 1.0 μg/ml) from eBioscience; and anti-CD11c (clone N418, 2.5 μg/ml) from BioLegend.
Bergot A.S., Monnet N., Tran L.S., Mittal D., Al-Kouba J., Steptoe R.J., Grimbaldeston M.A., Frazer I.H, & Wells J.W. (2015). HPV16-E7 Expression in skin induces TSLP secretion, type 2 ILC infiltration and atopic dermatitis-like lesions. Immunology and cell biology, 93(6), 540-547.
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4

Identification and Quantification of ILC2 in BALF

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were analyzed by flow cytometry following staining with live/dead aqua dyes (Live/Dead fixable aqua dead cell stain kit, Invitrogen, 1/1000) and antibodies specific for the following markers: anti-TCRβ (clone H57-597, 5.0 μg/ml) from BioLegend; anti-CD45R/B220 (clone RA3-6B2, 1.0 μg/ml), anti-CD2 (clone RM2-5, 0.8 μg/ml), anti-CD25 (clone PC61, 1.0 μg/ml), anti-CD90.2 (clone 53-2.1, 2.5 μg/ml), and anti-CD11b (clone M1/70, 0.3 μg/ml) antibodies from BD Pharmingen; anti-CD45.2 (clone 104, 0.5 μg/ml) from eBioscience. ILC2 were gated as CD45+, CD2− TCRb− B220− CD11b−, CD90+ and CD25+ cells in a similar manner to that described by Roediger et al.10 (link). Bronchoalveolar lavage fluid (BALF) cells were additionally labeled using anti-Siglec F (clone E50-2440, 2.0 μg/ml), from BD Pharmingen; anti-Gr-1 (clone RB6-8C5, 0.4 μg/ml), anti-CD8 (clone 53-6.7, 5.0 μg/ml), anti-CD4 (clone GK1.5, 1.0 μg/ml), anti-CD3 (clone 2C11, 1.0 μg/ml) from eBioscience; and anti-CD11c (clone N418, 2.5 μg/ml) from BioLegend.
Bergot A.S., Monnet N., Tran L.S., Mittal D., Al-Kouba J., Steptoe R.J., Grimbaldeston M.A., Frazer I.H, & Wells J.W. (2015). HPV16-E7 Expression in skin induces TSLP secretion, type 2 ILC infiltration and atopic dermatitis-like lesions. Immunology and cell biology, 93(6), 540-547.
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