Pyrrolidine dithiocarbamate pdtc

Keratinocyte serum-free medium (KSFM), all the other medium power, Trizol reagent, Lipofectamine 2000, OPTI-MEM, anti-Myc epitope antibodies and zeocin were from Life Technologies (Grand Island, NY., USA). Endothelial growth medium 2 (EGM2) for growing endothelial cells was from Lonza Inc. (Allendale, NJ, USA). All the chemicals, anti-His tag antibodies and Wortmanin were from Sigma-Aldrich Co. (St. Louis, MO, USA). CellTiter 96^® AQueous One Solution (MTS kit) was from Promega Corp (Madison, WI, USA). pLKO_AS2.zeo was from National RNAi Core facility in Academia Sinica, Taiwan. Anti-CD15, anti-CD68, and anti-CD11b antibodies were from Leica Biosciences (Richmond, Illinois, USA). S100A9 antibodies and STAT3 inhibitor VI (iSTAT3) were from Santa Cruz Biotechnology (Dallas, Texas, USA). Anti-human CD34 antibody was from DakoCytomation Denmark A/S (Copenhagen, Denmark). Anti-S100A8 antibodies, human recombinant S100A9 (recS100A9) protein and anti-CD31 antibodies were from Abcam (Cambridge, MA, USA). Matrigel was from BD Biosciences (San Jose, CA, USA). Pyrrolidine dithiocarbamate (PDTC) was from Tocris Bioscience (Bristol, UK). AZD1480 was from Selleckchem (Houston, TX, USA).

TLR agonists LPS from Escherichia coli serotype 026:B6 (TLR4), Pam3Cys (TLR2) and Poly(I:C) (TLR3) were purchased from Sigma (St. Louis, MO), Calbiochem (San Diego, CA), and Invivogen (San Diego, CA), respectively. Methylcarbamyl PAF C-16 (cPAF, a more metabolically stable analog) was purchased from Cayman Chemical (Ann Arbor, MI). NF-κB inhibitors acetyl-L-leucyl-L-leucyl-L-norleucinal (ALLN) and pyrrolidinedithiocarbamate (PDTC) were obtained from Tocris (Minneapolis, MN). CREB inhibitor KG-501 (2-naphthol-AS-E-phosphate) and PPARγ antagonist GW9662 were obtained from Sigma (St. Louis, MO). NF-κB p105/p50 inhibitor peptide was obtained from Novus Biologicals (Littleton, CO). Anti-COX2 Ab was supplied by Cayman (Ann Arbor, MI). Anti-iNOS, anti-phospho-IκBα (Ser32), anti-phospho-NF-κB p65 (Ser536), anti-phospho STAT3 (Tyr705), anti-β-actin, and secondary Abs goat anti-rabbit IgG and goat anti-mouse IgG conjugated to HRP were purchased from Cell Signaling Technology (Danvers, MA). Anti phospho-NF-κB p105/p50 (Ser337) was from SAB Signaling (College Park, MD).