3.50 m calcium chloride cacl2 solution

ACP was prepared by adding 100 mL of 2.33 M sodium phosphate dibasic solution (Sigma-Aldrich, St. Louis, MO, USA) and 100 mL of 3.50 M calcium chloride (CaCl₂) solution (Sigma-Aldrich, St. Louis, MO, USA) into sodium hydroxide that was dissolved in 100 mL of distilled water at pH 9 as a buffering medium. ACP was then filtered by high-power filtration and was freeze-dried for two days in accordance with a previous study [38 (link)]. CSH was purchased from Sigma-Aldrich, USA.

ACP powder was made by combining 2.33 M sodium phosphate dibasic solution (Sigma-Aldrich, St. Louis, MO, USA) and a 3.50 M calcium chloride (CaCl₂) solution (Sigma-Aldrich) in sodium hydroxide, and then dissolving it in distilled water. CSH was obtained from Sigma-Aldrich in Germany.
At 120 °C, 1 wt% Alg (Sigma-Aldrich) and 0.9% HPMC (Sigma-Aldrich) were dissolved in phosphate-buffered saline (PBS). ACP and CSH were added at a ratio of 2:3 to the gel in various amounts, which resulted in 13%ACP/CSH, 15%ACP/CSH, 18%ACP/CSH, 20%ACP/CSH, and 23%ACP/CSH. A 0%ACP/CSH group served as the control. To achieve a homogeneous and desirable particle size, the printing ink dispersion was filtered through a 110 μm filter [22 (link)].