Proteins were subjected to SDS-PAGE and then transferred onto a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). The following primary antibodies were used: (1) an anti-dysbindin antibody [19 (link)], (2) a monoclonal anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), (3) an anti-Ub antibody (Santa Cruz Biotechnology), (4) an anti-HA antibody (Santa Cruz Biotechnology), (5) an anti-Flag antibody (Sigma), (6) an anti-p65 antibody (Abcam), (7) an anti-Histone H2B antibody (Abcam) and (8) a monoclonal anti-GAPDH antibody (Millipore). Anti-mouse IgG-HRP and anti-rabbit IgG-HRP antibodies (GE Healthcare, UK) were used as secondary antibodies. The proteins were visualized using a SuperSignal West Pico instrument (Thermo Scientific, Waltham, MA, USA).
Anti histone h2b antibody
Manufactured by Abcam
Sourced in United States
Anti-Histone H2B antibody is a primary antibody that specifically recognizes the histone H2B protein. Histones are nuclear proteins that play a fundamental role in the organization of DNA into chromatin within eukaryotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Monoclonal anti gapdh antibody, by Merck Group (1 mentions)
Anti mouse igg hrp, by GE Healthcare (1 mentions)
Anti gfp monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Anti flag antibody, by Merck Group (1 mentions)
Anti ha antibody, by Santa Cruz Biotechnology (1 mentions)
Anti ub antibody, by Santa Cruz Biotechnology (1 mentions)
Anti rabbit igg hrp, by GE Healthcare (1 mentions)
Anti p65 antibody, by Abcam (1 mentions)
Protein g agarose, by Roche (1 mentions)
Mitochondria isolation kit, by Thermo Fisher Scientific (1 mentions)
Ezview red anti ha affinity gel, by Merck Group (1 mentions)
Anti mtco1 antibody, by Abcam (1 mentions)
β actin, by Merck Group (1 mentions)
Anti flag m2 affinity gel, by Merck Group (1 mentions)
Anti ha beads, by Roche (1 mentions)
Anti atp5a1 antibody, by Abcam (1 mentions)
Ha 11 clone 16b12, by Fortrea (1 mentions)
2 protocols using anti histone h2b antibody
1
Immunoblotting analysis of cellular proteins
2
Mitochondria and Nucleus Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The Thermo Scientific Mitochondria Isolation Kit and Nucleus Isolation Kit were used for cell fraction isolation. The extracts were obtained and proteins were solubilized for immunoblotting. The following antibodies were used: HA.11 Clone 16B12 (Covance, MMS-101P), FLAG M2-Peroxidase (HRP) (sigma, M8592), beta-actin (sigma), Anti-ATP5A1 antibody (abcam, ab14748), MYBBP1A Antibody (santa cruz, sc-133800), Anti-PP2A Antibody (Millipore,05-421), Hsp60 (Pierce, MA3-012), Cdk1 Antibody (santa cruz, sc-53219), MSH2 Antibody (Novus, NB100-56428), Anti-Histone H2B antibody (Abcam, ab18977), GAPDH (14C10) (cell signaling, 3683), FANCD2 (novusbio, NB100-182), AIF Antibody (santa cruz, sc-5586), ATAD3A/B/C (santa cruz, sc-292156), EF-Tu (santa cruz, sc-367739), Anti-ALDH2 (sigma, SAB2501484), mtTFA (santa cruz, sc-23588), Anti-MTCO1 antibody (abcam, ab14705). EZview™ Red Anti-HA Affinity Gel (sigma, E6779-1ML), anti-HA beads (Roche), ANTI-FLAG® M2 Affinity Gel (Sigma, A2220-1ML), Protein G Agarose (Roche, 11719416001) were used for IP.
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