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Oct compound

Manufactured by Gentaur
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The OCT compound is a laboratory reagent used for various applications in scientific research and analysis. It is a chemical compound that serves as a core component in various analytical and experimental procedures. The specific details and intended uses of the OCT compound are not provided in this factual and unbiased description.

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Lab products found in correlation

Alexa fluor 488 conjugated anti rat antibody, by Thermo Fisher Scientific (2 mentions) Deadend fluorometric tunel system, by Promega (2 mentions) Oil red o, by Merck Group (2 mentions) Anti cd68 mab clone fa 11, by Bio-Rad (2 mentions) Anti ki67 pe conjugated mab clone 16a8, by BioLegend (2 mentions)

Spelling variants of this product

Tissue-Tek OCT compound (2 citations)

3 protocols using oct compound

1

Histological Analysis of Liver Steatosis

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Frozen liver samples from offspring were embedded in O.C.T. compound (Gentaur, Kampenhout, Belgium) prior to sectioning. All sections were then stained with hematoxylin and eosin (H&E) or oil red O (ORO, Newcomer Supply, Middleton, WI) and evaluated for steatosis, fat accumulation, and inflammation, by a pathologist blind to the identity of the experimental groups.
Zhang X., Xu G.B., Zhou D, & Pan Y.X. (2018). High-fat diet modifies expression of hepatic cellular senescence gene p16(INK4a) through chromatin modifications in adult male rats. Genes & Nutrition, 13, 6.
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2

Aortic Plaque Evaluation in Mice

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Mice were sacrificed and slowly perfused with 10ml of ice-cold PBS. The hearts and aortas were carefully excised and fixed in 4% paraformaldehyde containing 30% sucrose. The aortas were stained with Oil Red O (Sigma-O0625) to evaluate plaque neutral lipid content. The hearts were embedded in OCT compound (Gentaur) and stored at −80°C before analysis. 10μm cryosections of the aortic sinus were prepared. Oil Red O staining was used to detect neutral lipid content in the plaque combined with a haematoxylin/eosin staining to analyse tissue architecture. Plaque macrophages were visualized using purified anti-CD68 mAb (clone FA-11, AbD Serotec). Anti-rat Alexa Fluor 488-conjugated antibody (A-11006, Life technologies) was used for detection of CD68 staining. For analysis of plaque macrophage proliferation, anti-Ki67 PE conjugated mAb (clone 16A8, BioLegend) was used. Nuclei were revealed with DAPI counterstaining (2μg/ml). TUNEL staining was performed using the DeadEnd™ Fluorometric TUNEL System (Promega). Plaque area quantification were measured with ImageJ software.
Merlin J., Ivanov S., Dumont A., Sergushichev A., Gall J., Stunault M., Ayrault M., Vaillant N., Castiglione A., Swain A., Orange F., Gallerand A., Berton T., Martin J.C., Carobbio S., Masson J., Gaisler-Salomon I., Maechler P., Rayport S., Sluimer J.C., Biessen E.A., Guinamard R.R., Gautier E.L., Thorp E.B., Artyomov M.N, & Yvan-Charvet L. (2021). Non-canonical glutamine transamination sustains efferocytosis by coupling redox buffering to oxidative phosphorylation. Nature metabolism, 3(10), 1313-1326.
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3

Aortic Plaque Evaluation in Mice

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Mice were sacrificed and slowly perfused with 10ml of ice-cold PBS. The hearts and aortas were carefully excised and fixed in 4% paraformaldehyde containing 30% sucrose. The aortas were stained with Oil Red O (Sigma-O0625) to evaluate plaque neutral lipid content. The hearts were embedded in OCT compound (Gentaur) and stored at −80°C before analysis. 10μm cryosections of the aortic sinus were prepared. Oil Red O staining was used to detect neutral lipid content in the plaque combined with a haematoxylin/eosin staining to analyse tissue architecture. Plaque macrophages were visualized using purified anti-CD68 mAb (clone FA-11, AbD Serotec). Anti-rat Alexa Fluor 488-conjugated antibody (A-11006, Life technologies) was used for detection of CD68 staining. For analysis of plaque macrophage proliferation, anti-Ki67 PE conjugated mAb (clone 16A8, BioLegend) was used. Nuclei were revealed with DAPI counterstaining (2μg/ml). TUNEL staining was performed using the DeadEnd™ Fluorometric TUNEL System (Promega). Plaque area quantification were measured with ImageJ software.
Merlin J., Ivanov S., Dumont A., Sergushichev A., Gall J., Stunault M., Ayrault M., Vaillant N., Castiglione A., Swain A., Orange F., Gallerand A., Berton T., Martin J.C., Carobbio S., Masson J., Gaisler-Salomon I., Maechler P., Rayport S., Sluimer J.C., Biessen E.A., Guinamard R.R., Gautier E.L., Thorp E.B., Artyomov M.N, & Yvan-Charvet L. (2021). Non-canonical glutamine transamination sustains efferocytosis by coupling redox buffering to oxidative phosphorylation. Nature metabolism, 3(10), 1313-1326.
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