Frame sealed incubation chambers

Cyclic olefin copolymers (COC 8007, 6013 and 6017) were purchased from TOPAS Advanced Polymers (Florence KY) in either 1.5 mm or 100 μm thick sheets. Polyethylene, PE, was purchased from Goodfellow (Coraopolis, PA). Frame-sealed incubation chambers were purchased from Bio-Rad (Hercules, CA). Chemicals and reagents used in these studies included sodium carbonate and bicarbonate (Fisher Scientific, Houston, TX); acetic acid (Sigma-Aldrich, St. Louis, MO), and toluidine blue O (TBO; Carolina Biological Supply, Burlington, NC).

PMMA and 6013S-04 COC were purchased from Plaskolite (Columbus, OH) and TOPAS Advanced Polymers (Florence, KY), respectively. Frame-sealed incubation chambers were purchased from Bio-Rad (Hercules, CA). Chemicals and reagents used in these studies included: Micro-90, sodium dodecyl sulfate (SDS), sodium hydroxide, sodium carbonate and bicarbonate (Fisher Scientific, Houston, TX); reagent-grade isopropyl alcohol (IPA), 2-(4-morpholino)-ethane sulfonic acid (MES), bovine serum albumin (BSA), Triton X-100, and paraformaldehyde solution (PFA) (Sigma-Aldrich, St. Louis, MO); toluidine blue O (TBO) (Carolina Biological Supply, Burlington, NC); phosphate-buffered saline (PBS, pH = 7.4) (Life Technologies, Carlsbad, CA); 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) (Pierce, Rockford, IL); Cy3-labeled oligonucleotides (5′NH₂-C₆-TTT-TTT-TTT-TTC-CGA-CAC-TTA-CGT-TTT-TTT-T-Cy3-3′; Integrated DNA Tech., Coralville, IA); NorthernLights 493-streptavidin (NL493-streptavidin), monoclonal anti-EpCAM Abs, and Fc blocker IgG (R&D Systems, Minneapolis, MN); and 4′,6-diamidino-2-phenylindole (DAPI), and CD45-FITC and cytokeratin 8 and 19 (CK8/19-PK) Abs (eBioscience, San Diego, CA). Nuclease-free water and microtubes (Ambion, Foster City, CA) were used for preparation and storage of all samples and reagents unless noted otherwise.