Lc ms 8030 tandem mass spectrometer

Following instruments were used to carry out the physical and spectral data: IR spectra on Perkin Elemer FT-IR spectrometer as KBr Pellet or neat sample. NMR spectra on 500, 400 and 125 MHz Bruker spectrometers in CDCl₃ with TMS as internal standard, chemical shift is expressed in δ (ppm) and coupling constant in Hertz. Mass analysis was carried out using Nexera UHPLC @ 130 Mpa with SIL-30 AC Nexera autosampler coupled to an LC–MS 8030 tandem mass spectrometer manufactured by Shimadzu Corporation, Kyoto, Japan. All the compounds were analysed in full scan mode with nitrogen servin gas an interface gas. Detection was done in + APCI mode having probe voltage of 180.0 V, with probe temperature of 400°C and nebulising gas flow of 2.5 L/min. Column chromatography was carried on normal phase silica gel 60–120 mesh (Merck grade), precoated TLC plates with silica gel 60 F254(Merck, 0.25 mm). Detection was done by using UV torch, iodine vapours and cerric sulphate.

Analysis of amino acids and acylcarnitines in the NS DBS was performed using the flow injection method with an LCMS-8030 tandem mass spectrometer (Shimadzu, Kyoto, Japan). The second-tier measurement of MMA and tHcy in DBS was performed using liquid chromatography–mass spectrometry with an LCMS-8040 tandem mass spectrometer (Shimadzu). Cutoff values for MMA and tHcy were 1 μmol/L and 5 μmol/L, respectively.