Lv100pol

Manufactured by Nikon

Sourced in Japan

The LV100POL is a polarizing microscope designed for laboratory use. It provides the core function of enabling the observation and analysis of birefringent samples through the use of polarized light.

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Lab products found in correlation

Ds ri1, by Nikon (2 mentions) Deoxyribonucleic acid sodium salt from salmon testes, by Merck Group (1 mentions) Acridine orange, by Merck Group (1 mentions) C2 plus, by Nikon (1 mentions) Tst350, by Linkam (1 mentions) Em uc7, by Leica camera (1 mentions) Epon resin, by Ted Pella (1 mentions) Jem 1011, by JEOL (1 mentions) Su8230, by Hitachi (1 mentions) Afg3022, by Tektronix (1 mentions) Eos 800d, by Canon (1 mentions) Thmsg600, by Linkam (1 mentions) Spectramax plus 384, by Molecular Devices (1 mentions) Usb2000 spectrometer, by OceanOptics (1 mentions) S 4800, by Hitachi (1 mentions) Multimode 8, by Bruker (1 mentions) Pma 11, by Hamamatsu Photonics (1 mentions) Pma 12, by Hamamatsu Photonics (1 mentions) Eclipse, by Nikon (1 mentions)

12 protocols using lv100pol

DNA Film Fabrication and Characterization

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DNA (Deoxyribonucleic acid sodium salt from salmon testes, Sigma Aldrich) was dissolved in deionised water at 25 mg/ml without further purification. Pristine silicon, micropost-patterned silicon, or glass substrates were rinsed with acetone, ethanol and deionised water and then treated with O₂ plasma for 5 min to eliminate any organic/inorganic impurities.
For the doctor blade experiment, micropost-patterned silicon and glass substrates were sandwiched with a gap using an aluminium foil of 40 μm thickness. The DNA solution was injected into the sandwich cell by capillary forces, and then the upper glass piece of the sandwich cell was pulled in the heating stage (Linkam TST350). The temperature range and pulling speeds were 25 °C and 3 μm/s, respectively.
The optical textures of the fabricated DNA film were directly observed by POM (Nikon LV100POL) using a first-order retardation plate (λ = 530 nm). Fluorescent confocal polarising microscopy (C2 plus, Nikon) with a linearly polarised laser source (λ = 488 nm) was used to observe the meniscus line of the DNA microstructures. For this experiment, the DNA solution was mixed with a fluorescent dye molecule, Acridine orange (Aldrich).

Cha Y.J., Park S.M., You R., Kim H, & Yoon D.K. (2019). Microstructure arrays of DNA using topographic control. Nature Communications, 10, 2512.

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Cryogenic Characterization of CCNF Hydrogels

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CCNF, CCNF-DA, and CCNF-DA/AgNPs hydrogels with the concentration of 1 (w/v) % were analyzed by a cryogenic transmission electron microscopy (cryo-TEM; JEOL JEM 1011, Tokyo, Japan) and a polarized optical microscopy (POM; Nikon LV100 Pol) before the film casting. The samples were covered with the flat side of another B-type planchette and were rapidly frozen in a Bal-Tec HPM 010 high-pressure freezer (Boeckeler Instruments, Tucson, AZ, USA). After freeze substitution for 5 days at −80 °C in anhydrous acetone containing 2% OsO₄, the samples were warmed up to room temperature over 2 days (24 h from −80 °C to −20 °C, 20 h from −20 °C to 4 °C, 4 h from 4 °C to 20 °C). After washing 3 times with anhydrous acetone, the samples were embedded in a graduated Epon resin (Ted Pella Inc., Redding, CA, USA) and diluted in acetone (5%, 15%, 25%, 50%, 75% and 100% (v/v)) over 3 days. After polymerization in a 60 °C oven for 24 h, the samples were sectioned and post-stained with aqueous 2% (v/v) uranyl acetate (UA) and Reynolds lead citrate (LC) solution. The resin was cut into 200 nm-sections using Leica EM UC7 (Wetzlar, Germany) and applied onto copper grids. The TEM images of samples were recorded. The optical textures were characterized by depolarized transmitted light microscopy (DTLM).

Nguyen H.L., Jo Y.K., Cha M., Cha Y.J., Yoon D.K., Sanandiya N.D., Prajatelistia E., Oh D.X, & Hwang D.S. (2016). Mussel-Inspired Anisotropic Nanocellulose and Silver Nanoparticle Composite with Improved Mechanical Properties, Electrical Conductivity and Antibacterial Activity. Polymers, 8(3), 102.

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Optical and Electrical Characterization of MXene

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The optical textures of the MXene sample were observed using POM (LV100POL, Nikon) with a CCD camera (DS-Ri1, Nikon). Scanning electron microscopy (SEM) (SU8230, Hitachi) was used to observe the morphology of the freeze-dried MXene sheets. Electrical property was determined by obtaining an I-V curve via voltage sweep from −1 V to 1 V between the two terminals using a semi-auto probe station (MPS20, Keysight).

Lee C., Park S.M., Kim S., Choi Y.S., Park G., Kang Y.C., Koo C.M., Kim S.J, & Yoon D.K. (2022). Field-induced orientational switching produces vertically aligned Ti3C2Tx MXene nanosheets. Nature Communications, 13, 5615.

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Thermal Stability and Electro-Optical Characterization

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Thermal stability of such PSBPLC sample was confirmed through the optical texture observed by a polarized optical microscope (POM, Nikon LV100POL) during a heating-and-cooling cycle with a settled rate of 0.5 °C/min. The probe light generated by a double-frequency neodymium-doped yttrium aluminium garnet (Nd:YAG) laser (532 nm, 10 mW/cm²) impinged on the sample along the cell normal for testing EO performances. The polarization direction of the probe beam was modulated to ± 45° with respect to the orientation of IPS electrodes, to ensure a larger transmission contrast between applying and removing the driving voltage (square wave, 1 kHz) generated from a signal generator (Tektronix, AFG3022). Kerr constant of the sample was obtained by fitting the dependency between the square of applied electric-field and the field-induced birefringence of the sample in accordance with the extended Kerr equation^{34 (link)}; while the birefringence was measured through the commonly used Senarmont’s method^{35 (link)}. The response behaviour was monitored by a photoelectric-converter-connected oscilloscope.

Li X., Yang W.Q., Yuan C.L., Liu Z., Zhou K., Wang X.Q., Shen D, & Zheng Z.G. (2017). Enhanced Low-temperature Electro-optical Kerr Effect of Stable Cubic Soft Superstructure Enabled by Fluorinated Polymer Stabilization. Scientific Reports, 7, 10383.

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Sedimentology and Conodont Biostratigraphy of Rock Samples

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In this study, 56 rock samples were collected from the Shangsi and Beifengjing sections for sedimentological and paleontological research. The rock samples were made into thin sectionsand photographed using an optical microscope (Nikon Lv100 pol).
In addition, 18 rock samples, each weighing approximately 3 kg, were collected from the Beifengjing section for conodont biostratigraphy research. The process of obtaining conodont samples is as follows: 1) The collected rock samples were crushed into particles with a diameter of 1–2 cm indoors, placed in plastic containers, labeled according to field records, and then soaked in 10–20 % industrial acetic acid. The samples were rinsed and the acid was replaced approximately every week for approximately five to six cycles. Subsequently, the acid-insoluble residues were sieved using a 160-mesh sieve and then dried in an experimental oven at 80 °C–90 °C. 2) The dried residues were examined under a binocular microscope to select conodonts. These conodonts were then fixed on carrier plates previously coated with a thin layer of latex using a brush. Finally, the carrier plates were placed under a scanning electron microscope (SEM) for imaging of each sample. All the above work was conducted at the State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation at Chengdu University of Technology.

Duan X, & Shi Z. (2024). Sedimentary records of sea level fall during the end-Permian in the upper Yangtze region (southern China): Implications for the mass extinction. Heliyon, 10(10), e31226.

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Optical Texture Visualization using POM

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Direct visualization of optical textures was carried out by POM (LV100POL, Nikon) equipped with a charge-coupled device (CCD) camera (DS-Ri1, Nikon). The temperature was controlled with the heating stage (Linkam LTS420) and a temperature controller (Linkam TMS94).

Chae H., Lee Y.H., Yang M., Yoon W.J., Yoon D.K., Jeong K.U., Song Y.H., Choi U.H, & Lee M. (2019). Interesting phase behaviors and ion-conducting properties of dicationic N-alkylimidazolium tetrafluoroborate salts. RSC Advances, 9(7), 3972-3978.

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Fiber Diameter Analysis Protocol

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Ten fibres were randomly taken from each stem slice, and six different positions were observed for each fiber. The diameter of fibres was measured with a polarised light microscope (Nikon LV100POL, Tokyo, Japan) at 100× magnification, and the average value and coefficient of variation were calculated.

Yu X., Xia Y., Liang D., Fu W, & Yin C. (2022). Effect of Warm-Water Retting Pretreatment on the Physical Properties of Banana Stem and Its Fibre. Materials, 15(23), 8462.

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Self-Shaping Process Microscopy

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The cell gap was adjusted with polymer spacers and held at 40 °C before the microscopic observation. The cell was sealed with 2 min epoxy. A Linkam THMSG600 heating stage was used to control the temperature of the cell. In this way, we followed the complete self-shaping process. We used a color camera (Canon, EOS 800D) mounted on the polarized optical microscope (Nikon LV 100POL) for observing the self-shaping process.

Peddireddy K., Čopar S., Le K.V., Muševič I., Bahr C, & Jampani V.S. (2021). Self-shaping liquid crystal droplets by balancing bulk elasticity and interfacial tension. Proceedings of the National Academy of Sciences of the United States of America, 118(14), e2011174118.

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Characterization of Liquid Crystal Films

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Polarized optical
microscopy (POM) (LV100POL, Nikon, Tokyo, Japan) was used to examine
the optical textures of LC film at different temperatures. The birefringence
intensity of the aligned LC films was quantitatively measured using
a UV–vis spectroscopy (SPECTRA max Plus 384, Molecular Devices,
Sunnyvale, CA) with a light source at 540 nm, in which the sandwich
cells were rotated.

Han M.J., Wei D., Kim Y.H., Ahn H., Shin T.J., Clark N.A., Walba D.M, & Yoon D.K. (2018). Highly Oriented Liquid Crystal Semiconductor for Organic Field-Effect Transistors. ACS Central Science, 4(11), 1495-1502.

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Characterization of Scratched Substrates

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The AFM images of the scratched substrates were obtained in contact mode (Bruker, Multimode-8). All polarised optical images were obtained using POM (LV100POL, Nikon). The transmittance of the scratched substrates and the TN cell was measured using a customized optical microscope equipped with a USB-2000+ spectrometer (Ocean Optics). The electric field was generated by a function generator (33210 A, Agilent). Assembled gold nanorods were directly observed using a field-emission scanning electron microscope (FE-SEM; S-4800, HITACHI) after a 2 nm-thick platinum coating.

Suh A, & Yoon D.K. (2018). Nanoscratching technique for highly oriented liquid crystal materials. Scientific Reports, 8, 9460.

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