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2 protocols using rabbit anti myc sc 764

1

Western Blot Analysis of Cell Lysates

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Cells were lysed using SDS sample buffer and protein concentrations were measured using Bio-Rad Protein Assay Dye (Bio-Rad 5000006). Proteins (~50 μg per sample) were separated on SDS-polyacrylamide gels, transferred to nitrocellulose membranes (926–31092, LI-COR Biosciences, Lincoln, NE), and probed with rabbit anti-cyclin E1 (ab33911, Abcam, Cambridge, MA), rabbit anti-GLDC (HPA002318, Sigma-Aldrich), mouse anti-MYCN (clone OP13, Millipore Sigma), rabbit anti-MYC (sc-764, Santa Cruz Biotech, Dallas, TX), rabbit anti-GAPDH (sc-25778, Santa Cruz Biotech), and mouse anti-α-tubulin (T5168, Sigma-Aldrich) antibodies. Horseradish Peroxidase conjugated goat anti-mouse (sc-2005, Santa Cruz Biotech) and goat anti-rabbit IgG (sc2004, Santa Cruz Biotech) were used as secondary antibodies to visualize proteins by chemiluminescence. For visualization with the Odyssey system (LI-COR Biosciences), Goat anti-mouse IRDye 800 (926–32210) or 680 (926–32220) and anti-rabbit IRDye 800 (926–32234) or 680 (926–68021) from LI-COR Biosciences were used as secondary antibodies.
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2

Western Blot Analysis of Cell Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed using SDS sample buffer and protein concentrations were measured using Bio-Rad Protein Assay Dye (Bio-Rad 5000006). Proteins (~50 μg per sample) were separated on SDS-polyacrylamide gels, transferred to nitrocellulose membranes (926–31092, LI-COR Biosciences, Lincoln, NE), and probed with rabbit anti-cyclin E1 (ab33911, Abcam, Cambridge, MA), rabbit anti-GLDC (HPA002318, Sigma-Aldrich), mouse anti-MYCN (clone OP13, Millipore Sigma), rabbit anti-MYC (sc-764, Santa Cruz Biotech, Dallas, TX), rabbit anti-GAPDH (sc-25778, Santa Cruz Biotech), and mouse anti-α-tubulin (T5168, Sigma-Aldrich) antibodies. Horseradish Peroxidase conjugated goat anti-mouse (sc-2005, Santa Cruz Biotech) and goat anti-rabbit IgG (sc2004, Santa Cruz Biotech) were used as secondary antibodies to visualize proteins by chemiluminescence. For visualization with the Odyssey system (LI-COR Biosciences), Goat anti-mouse IRDye 800 (926–32210) or 680 (926–32220) and anti-rabbit IRDye 800 (926–32234) or 680 (926–68021) from LI-COR Biosciences were used as secondary antibodies.
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