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E cadherin decma 1

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E-Cadherin (DECMA-1) is a laboratory reagent used in cell biology research. It is a monoclonal antibody that binds to the E-Cadherin protein, which is a cell-cell adhesion molecule involved in maintaining the integrity of epithelial cell layers.

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Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) Lsm 510 confocal microscope, by Zeiss (2 mentions) β catenin h 102, by Santa Cruz Biotechnology (2 mentions) Topro3 and slowfade diamond antifade with dapi, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

E-cadherin (534 citations) DECMA-1 (2 citations)

2 protocols using e cadherin decma 1

1

Immunofluorescence Staining of Cell Markers

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Frozen tissue samples or plated cells were fixed for 20 min with 35% methanol/65% acetone on ice. The samples were rehydrated with ice-cold PBS, permeabilized, blocked with 5% goat serum and 0.3% Triton X-100, then incubated with primary antibodies, CD177 (Clone 4C4, Sigma Aldrich), β-Catenin (H-102; Santa Cruz Biotechnology), or E-Cadherin (DECMA-1; Santa Cruz Biotechnology) overnight. The slides were then counter stained with ToPro3 and SlowFade diamond antifade with DAPI (Life Technologies). Images were taken using a Zeiss LSM710 or LSM510 confocal microscope.
Kluz P.N., Kolb R., Xie Q., Borcherding N., Liu Q., Luo Y., Kim M.C., Wang L., Zhang Y., Li W., Stipp C., Gibson-Corley K.N., Zhao C., Qi H.H., Bellizzi A., Tao A.W., Sugg S., Weigel R.J., Zhou D., Shen X, & Zhang W. (2020). Cancer cell-intrinsic function of CD177 in attenuating β-Catenin signaling. Oncogene, 39(14), 2877-2889.
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2

Immunofluorescence Staining of Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen tissue samples or plated cells were fixed for 20 min with 35% methanol/65% acetone on ice. The samples were rehydrated with ice-cold PBS, permeabilized, blocked with 5% goat serum and 0.3% Triton X-100, then incubated with primary antibodies, CD177 (Clone 4C4, Sigma Aldrich), β-Catenin (H-102; Santa Cruz Biotechnology), or E-Cadherin (DECMA-1; Santa Cruz Biotechnology) overnight. The slides were then counter stained with ToPro3 and SlowFade diamond antifade with DAPI (Life Technologies). Images were taken using a Zeiss LSM710 or LSM510 confocal microscope.
Kluz P.N., Kolb R., Xie Q., Borcherding N., Liu Q., Luo Y., Kim M.C., Wang L., Zhang Y., Li W., Stipp C., Gibson-Corley K.N., Zhao C., Qi H.H., Bellizzi A., Tao A.W., Sugg S., Weigel R.J., Zhou D., Shen X, & Zhang W. (2020). Cancer cell-intrinsic function of CD177 in attenuating β-Catenin signaling. Oncogene, 39(14), 2877-2889.
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