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20 70 dichlorofluorescein

Manufactured by Merck Group
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20,70-dichlorofluorescein is a chemical compound used in laboratory settings. It is a fluorescent dye that can be utilized for various analytical and research purposes. The core function of this product is to serve as a fluorescent marker or indicator in a range of applications, such as cell imaging, biomolecular labeling, and detection techniques. This description is presented in a factual and unbiased manner, without extrapolation on its intended use.

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Lab products found in correlation

Fluorescein sodium salt, by Merck Group (1 mentions) Quercetin, by Merck Group (1 mentions) Sulfuric acid h2so4, by Merck Group (1 mentions) 6 hydroxy 2 5 7 8 tetramethyl 2 carboxylic acid, by Merck Group (1 mentions) 20 70 dichlorofluorescin diacetate, by Merck Group (1 mentions) 2 20 azobis 2 amidino propane dihydrochloride aaph, by Merck Group (1 mentions) Ensight, by PerkinElmer (1 mentions)

Close spelling variants of this product

20,70-dichlorofluorescein diacetate (4 citations) 20,70-dichlorofluorescein diacetate (DCFH-DA) (2 citations)

2 protocols using 20 70 dichlorofluorescein

1

Antioxidant Capacity Evaluation Protocol

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Sodium carbonate anhydrous (Na2CO3), Folin-Ciocalteu reagent, sodium nitrite solution (NaNO2), aluminum chloride hexahydrate solution (AlCl3, 6 H2O), vanillin, catechin and gallic acid were purchased from Fluka (Buchs, Switzerland). Sulfuric acid (H2SO4) was obtained from Merck (Darmstadt, Germany). Fluorescein sodium salt (FL), 20,70 dichlorofluorescin-diacetate (DCFH-DA), 20,70-dichlorofluorescin (DCFH), 20,70-dichlorofluorescein (DCF), 6-hydroxy-2,5,7,8-tetramethyl-2-carboxylic acid, tert-butyl hydroperoxide (t-BuOOH), (Trolox), quercetin, and 2,20-azobis(2-amidino-propane) dihydrochloride (AAPH) were all purchased from Sigma-Aldrich (Oakville, ON). All the solvents were purchased from EMD (Canada).
Karker M., Falleh H., Msaada K., Smaoui A., Abdelly C., Legault J, & Ksouri R. (2016). Antioxidant, anti-inflammatory and anticancer activities of the medicinal halophyte Reaumuria vermiculata. EXCLI Journal, 15, 297-307.
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2

Measurement of Keratinocyte Oxidative Stress

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Keratinocytes were cultured in normal medium, detached at 80e90% of confluence, washed twice in PBS, and seeded on a microplate of 96 wells at the density of 10 5 per well. Cells were incubated for 30 minutes at 37 C with 5 mM of 2 0 ,7 0 -dichlorofluorescein (Sigma Aldrich, Saint Louis, MO). The fluorescence was detected at the Multimode Plate Reader EnSIGHT Perkin Elmer.
Cordisco S., Tinaburri L., Teson M., Orioli D., Cardin R., Degan P., Stefanini M., Zambruno G., Guerra L, & Dellambra E. (2019). Cockayne Syndrome Type A Protein Protects Primary Human Keratinocytes from Senescence. The Journal of investigative dermatology, 139(1).
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