Polyclonal rabbit anti phospho erbb2

Manufactured by Cell Signaling Technology

Polyclonal rabbit anti-phospho-ErbB2 is an antibody reagent that specifically binds to the phosphorylated form of the ErbB2 protein.

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ErbB2 (46 citations) Anti-ErbB2 (16 citations) Phospho-ERBB2 (13 citations) Rabbit anti-ErbB2 (2 citations)

2 protocols using polyclonal rabbit anti phospho erbb2

Evaluating Receptor Tyrosine Kinase Signaling

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The antibodies used are as follows: monoclonal mouse anti-actin (691001; MP Biomedicals, Santa Ana, CA); polyclonal rabbit anti-ErbB2 (HER2/neu), and monoclonal mouse anti-GFP (sc-284 and sc-9996, respectively; Santa Cruz Biotechnology, Dallas, TX); polyclonal rabbit anti-phospho-ErbB2 (2249; Cell Signaling Technology, Danvers, MA); polyclonal rabbit anti-GFP (632460; Clontech); and monoclonal mouse anti-BrdU (11170376001; Roche).
Tyrphostin AG-825 (10010243) was from Cayman Chemical. The aptamer GroA (AS1411) and the inactive control oligomer Cro, were purchased from IDT (Jerusalem, Israel) as unmodified desalted oligonucleotides, as previously described^{44 (link)}.

Wolfson E., Solomon S., Schmukler E., Goldshmit Y, & Pinkas-Kramarski R. (2018). Nucleolin and ErbB2 inhibition reduces tumorigenicity of ErbB2-positive breast cancer. Cell Death & Disease, 9(2), 47.

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Investigating ErbB2 and Related Signaling

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The antibodies used are as follows: monoclonal mouse anti-actin (691001; MP Biomedicals, Santa Ana, CA); polyclonal rabbit anti-ErbB2 (HER2/neu), rabbit anti-nucleolin (C23), rabbit anti-Erk2 and rabbit anti-Akt, monoclonal mouse anti-phosphotyrosine (pY20) and monoclonal mouse anti-GFP and mouse anti-nucleolin (sc-284; sc-13057; sc-154; sc-8312; sc-508; sc-9996; sc-8031, respectively; Santa Cruz Biotechnology, Dallas, TX); monoclonal mouse anti-tubulin (T7816; Sigma-Aldrich); polyclonal rabbit anti-phospho-ErbB2, rabbit anti-phospho-Akt, rabbit anti-phospho-Erk1/2 (2249; 4058; 9101, respectively; Cell Signaling Technology, Danvers, MA); and monoclonal mouse anti-ErbB2 (extracellular; L87), which was a gift from Prof. Y. Yarden, Weizmann Institute of Science, Israel.
The aptamer GroA (AS1411) and the inactive oligomer Cro, were purchased from IDT (Jerusalem, Israel) as unmodified desalted oligonucleotides, as previously described [38 (link)].

Wolfson E., Goldenberg M., Solomon S., Frishberg A, & Pinkas-Kramarski R. (2016). Nucleolin-binding by ErbB2 enhances tumorigenicity of ErbB2-positive breast cancer. Oncotarget, 7(40), 65320-65334.

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