Adult skin tissue samples were obtained from donors undergoing elective abdominal dermolipectomy (age 25–58 years, BMI <30, n = 77) with no objection certificate according to the bioethic law no. 2004–800 of August 6, 2004. Keratinocytes were isolated after epidermis and dermis digestion with 10 mg/ml dispase solution (Sigma Aldrich) in Hank’s Balanced Salt Solution (HBSS, ThermoFisher) then trypsine solution (Life technologies) to dissociate epidermis after filtration (100 µm, EMD Millipore). Keratinocytes were cultivated in Dermalife K medium (Cell System) supplemented with 1% ASP. Skin fibroblasts were obtained using dermis explants cultivated in α-MEM medium (Life technologies) with 1% ASP and 20% FBS. Adipose Stromal Cells (ASC) were obtained by digestion of subcutaneous adipose tissue with 0,4 U/ml collagenase NB4 (SERVA electrophoresis) solution in α-MEM medium then removal of floating adipocytes to isolate and culture the stromal vascular fraction in α-MEM medium with 1% ASP and 10% FBS. Cells were used from passage 0 to 4 (P0 to P4).
Bourdens M., Jeanson Y., Taurand M., Juin N., Carrière A., Clément F., Casteilla L., Bulteau A.L, & Planat-Bénard V. (2019). Short exposure to cold atmospheric plasma induces senescence in human skin fibroblasts and adipose mesenchymal stromal cells. Scientific Reports, 9, 8671.
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